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AB137679

Anti-ornithine aminotransferase antibody

5

(1 Review)

|

(11 Publications)

Rabbit Polyclonal ornithine aminotransferase antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OAT aa 1-400.

View Alternative Names

Ornithine delta-aminotransferase, Ornithine--oxo-acid aminotransferase, OAT

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-ornithine aminotransferase antibody (AB137679)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ornithine aminotransferase antibody (AB137679)

Immunofluorescence analysis of methanol-fixed A431 cells labelling ornithine aminotransferase with ab137679 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ornithine aminotransferase antibody (AB137679)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ornithine aminotransferase antibody (AB137679)

Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue, labelling ornithine aminotransferase with ab137679 at 1/100 dilution.

Western blot - Anti-ornithine aminotransferase antibody (AB137679)
  • WB

Unknown

Western blot - Anti-ornithine aminotransferase antibody (AB137679)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ornithine aminotransferase antibody (ab137679) at 1/1000 dilution

All lanes:

293T whole cell lysate at 30 µg

Predicted band size: 49 kDa

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Western blot - Anti-ornithine aminotransferase antibody (AB137679)
  • WB

Unknown

Western blot - Anti-ornithine aminotransferase antibody (AB137679)

10% SDS PAGE

All lanes:

Western blot - Anti-ornithine aminotransferase antibody (ab137679) at 1/1000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 49 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human OAT aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

P04181

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>or Tris-EDTA buffer (pH8.0)</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ornithine aminotransferase also known as ornithine transaminase is an enzyme that facilitates the conversion of ornithine and α-ketoglutarate to glutamate semialdehyde and glutamate. This enzyme weighs around 49 kDa. It shows expression mainly in the liver kidney small intestine and retina. The enzyme works inside cellular mitochondria and assists by catalyzing amino group transfer reactions. It is one member of the transaminase family.
Biological function summary

This enzyme plays a role in the urea cycle and amino acid metabolism. It helps in the conversion of excess nitrogen into less toxic forms that the body can excrete. As a part of an important metabolic network it influences the synthesis and degradation of important biological molecules. It operates independently and does not form known protein complexes for its function.

Pathways

Ornithine aminotransferase is important in the ornithine and glutamate metabolism pathways. Within the urea cycle it cooperates with enzymes such as arginase and carbamoyl phosphate synthetase to regulate nitrogen balance in cells. Additionally it engages in the proline-glutamate pathway which involves proteins like proline dehydrogenase analzying the reversible conversion between proline and glutamate.

Ornithine aminotransferase associates with gyrate atrophy a rare genetic condition that affects the eye and leads to vision loss. Deficiencies in this enzyme can result in abnormal accumulations of ornithine in the blood. Some connections also exist with hyperammonemia where impaired nitrogen excretion causes elevated ammonia levels. In these conditions the enzyme's defective activity links to mutations or irregular interactions with proteins like glutamate dehydrogenase affecting metabolic balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the reversible interconversion of L-ornithine and 2-oxoglutarate to L-glutamate semialdehyde and L-glutamate.
See full target information OAT
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Nature 633:895-904 PubMed39169180

2024

Short-term post-fast refeeding enhances intestinal stemness via polyamines.

Applications

Unspecified application

Species

Unspecified reactive species

Shinya Imada,Saleh Khawaled,Heaji Shin,Sven W Meckelmann,Charles A Whittaker,Renan Oliveira Corrêa,Chiara Alquati,Yixin Lu,Guodong Tie,Dikshant Pradhan,Gizem Calibasi-Kocal,Luiza Martins Nascentes Melo,Gabriele Allies,Jonas Rösler,Pia Wittenhofer,Jonathan Krystkiewicz,Oliver J Schmitz,Jatin Roper,Marco Aurelio Ramirez Vinolo,Luigi Ricciardiello,Evan C Lien,Matthew G Vander Heiden,Ramesh A Shivdasani,Chia-Wei Cheng,Alpaslan Tasdogan,Ömer H Yilmaz

Experimental & molecular medicine 56:478-490 PubMed38413821

2024

Role of lung ornithine aminotransferase in idiopathic pulmonary fibrosis: regulation of mitochondrial ROS generation and TGF-β1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Jong-Uk Lee,Ki Sung Song,Jisu Hong,Hyesun Shin,Eunji Park,Junyeong Baek,Shinhee Park,Ae-Rin Baek,Junehyuk Lee,An Soo Jang,Do Jin Kim,Su Sie Chin,U-Jin Kim,Sung Hwan Jeong,Sung-Woo Park

EMBO molecular medicine 15:e17033 PubMed36647689

2023

Liver-directed gene therapy for ornithine aminotransferase deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Iolanda Boffa,Elena Polishchuk,Lucia De Stefano,Fabio Dell'Aquila,Edoardo Nusco,Elena Marrocco,Matteo Audano,Silvia Pedretti,Marianna Caterino,Ilaria Bellezza,Margherita Ruoppolo,Nico Mitro,Barbara Cellini,Alberto Auricchio,Nicola Brunetti-Pierri

Nature chemical biology 19:292-300 PubMed36280791

2022

Hepatic glutamine synthetase controls N-methylglutamine in homeostasis and cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Victor H Villar,Maria Francesca Allega,Ruhi Deshmukh,Tobias Ackermann,Mark A Nakasone,Johan Vande Voorde,Thomas M Drake,Janina Oetjen,Algernon Bloom,Colin Nixon,Miryam Müller,Stephanie May,Ee Hong Tan,Lars Vereecke,Maude Jans,Gillian Blancke,Daniel J Murphy,Danny T Huang,David Y Lewis,Thomas G Bird,Owen J Sansom,Karen Blyth,David Sumpton,Saverio Tardito

Nature communications 11:6127 PubMed33257663

2020

Lkb1 suppresses amino acid-driven gluconeogenesis in the liver.

Applications

Unspecified application

Species

Unspecified reactive species

Pierre-Alexandre Just,Sara Charawi,Raphaël G P Denis,Mathilde Savall,Massiré Traore,Marc Foretz,Sultan Bastu,Salimata Magassa,Nadia Senni,Pierre Sohier,Maud Wursmer,Mireille Vasseur-Cognet,Alain Schmitt,Morgane Le Gall,Marjorie Leduc,François Guillonneau,Jean-Pascal De Bandt,Patrick Mayeux,Béatrice Romagnolo,Serge Luquet,Pascale Bossard,Christine Perret

Molecular & cellular proteomics : MCP 19:1330-1345 PubMed32467259

2020

The Mouse Heart Mitochondria N Terminome Provides Insights into ClpXP-Mediated Proteolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Eduard Hofsetz,Fatih Demir,Karolina Szczepanowska,Alexandra Kukat,Jayachandran N Kizhakkedathu,Aleksandra Trifunovic,Pitter F Huesgen

Journal of inherited metabolic disease 42:1128-1135 PubMed30724386

2019

Hepatic glutamine synthetase augmentation enhances ammonia detoxification.

Applications

Unspecified application

Species

Unspecified reactive species

Leandro R Soria,Matthew Nitzahn,Angela De Angelis,Suhail Khoja,Sergio Attanasio,Patrizia Annunziata,Donna J Palmer,Philip Ng,Gerald S Lipshutz,Nicola Brunetti-Pierri

Cell 175:101-116.e25 PubMed30220459

2018

Transaminase Inhibition by 2-Hydroxyglutarate Impairs Glutamate Biosynthesis and Redox Homeostasis in Glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel K McBrayer,Jared R Mayers,Gabriel J DiNatale,Diana D Shi,Januka Khanal,Abhishek A Chakraborty,Kristopher A Sarosiek,Kimberly J Briggs,Alissa K Robbins,Tomasz Sewastianik,Sarah J Shareef,Benjamin A Olenchock,Seth J Parker,Kensuke Tateishi,Jessica B Spinelli,Mirazul Islam,Marcia C Haigis,Ryan E Looper,Keith L Ligon,Bradley E Bernstein,Ruben D Carrasco,Daniel P Cahill,John M Asara,Christian M Metallo,Neela H Yennawar,Matthew G Vander Heiden,William G Kaelin

American journal of physiology. Gastrointestinal a 315:G638-G649 PubMed30048597

2018

Prematurity reduces citrulline-arginine-nitric oxide production and precedes the onset of necrotizing enterocolitis in piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Jason L Robinson,Victoria A Smith,Barbara Stoll,Umang Agarwal,Muralidhar H Premkumar,Patricio Lau,Stephanie M Cruz,Rodrigo Manjarin,Oluyinka Olutoye,Douglas G Burrin,Juan C Marini

American journal of physiology. Endocrinology and 313:E233-E242 PubMed28611027

2017

The intestinal-renal axis for arginine synthesis is present and functional in the neonatal pig.

Applications

Unspecified application

Species

Unspecified reactive species

Juan C Marini,Umang Agarwal,Jason L Robinson,Yang Yuan,Inka C Didelija,Barbara Stoll,Douglas G Burrin
View all publications
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