Mouse Monoclonal Ornithine Carbamoyltransferase/OTC antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Ornithine transcarbamylase, mitochondrial aa 150-300.
IgG1
Mouse
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Monoclonal
IHC-P | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/5000.00000 - 1/10000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100 |
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Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline (PubMed:2556444, PubMed:6372096, PubMed:8112735). The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion (PubMed:2556444).
OTCase, OTC
Mouse Monoclonal Ornithine Carbamoyltransferase/OTC antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Ornithine transcarbamylase, mitochondrial aa 150-300.
IgG1
Mouse
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Liquid
Monoclonal
CL4046
Affinity purification Protein A
Binds to an epitope located within the peptide sequence GYEPDASVTKLAEQY as determined by overlapping synthetic peptides.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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All lanes: Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4046] (ab262865) at 1 µg/mL
All lanes: Human liver lysate
Predicted band size: 39 kDa
PFA-fixed, Triton X-100 permeabilized RH-30 cells stained for Ornithine Carbamoyltransferase/OTC (green) using ab262865 at 4 μg/ml in ICC/IF.
Paraffin-embedded human small intestine tissue stained for Ornithine Carbamoyltransferase/OTC using ab262865 at 1/5000 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for Ornithine Carbamoyltransferase/OTC using ab262865 at 1/5000 dilution in immunohistochemical analysis.
Paraffin-embedded human duodenum tissue stained for Ornithine Carbamoyltransferase/OTC using ab262865 at 1/5000 dilution in immunohistochemical analysis.
Paraffin-embedded human endometrium tissue stained for Ornithine Carbamoyltransferase/OTC using ab262865 at 1/5000 dilution in immunohistochemical analysis. No expression, as expected.
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