Mouse Monoclonal Ornithine Carbamoyltransferase/OTC antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Ornithine transcarbamylase, mitochondrial aa 150-300.
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
WB | |
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Human | Tested |
Species | Dilution info | Notes |
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Species Human | Dilution info 1 µg/mL | Notes - |
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Catalyzes the second step of the urea cycle, the condensation of carbamoyl phosphate with L-ornithine to form L-citrulline (PubMed:2556444, PubMed:6372096, PubMed:8112735). The urea cycle ensures the detoxification of ammonia by converting it to urea for excretion (PubMed:2556444).
OTCase, OTC
Mouse Monoclonal Ornithine Carbamoyltransferase/OTC antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Ornithine transcarbamylase, mitochondrial aa 150-300.
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Ornithine Carbamoyltransferase (OTC) also known as ornithine aminotransferase is an enzyme critical in the urea cycle. The OTC protein facilitates the conversion of ornithine and carbamoyl phosphate into citrulline a process essential for nitrogen excretion. The human OTC protein has a mass of approximately 36 kilodaltons and is primarily expressed in the liver mitochondria. Protein assays and procurement efforts often focus on obtaining accurate measurements and ensuring high-purity OTC peptides enabling proper functional studies.
Ornithine carbamoyltransferase plays a significant role in detoxifying ammonia by forming citrulline an important urea cycle intermediate. The protein functions as a homotrimer meaning three identical subunits form an active enzyme complex. This multimeric state facilitates efficient substrate processing allowing effective handling of nitrogen waste. The integral role of OTC in the urea cycle highlights its importance in maintaining systemic nitrogen balance.
OTC is a central component of the urea cycle and influences nitrogen metabolism pathways. These pathways include the conversion of ammonia to urea an important detoxifying process for safe excretion by the kidneys. OTC closely interacts with enzymes such as argininosuccinate synthetase highlighting the coordinated action required to advance urea formation. Understanding OTC's participation within these pathways helps identify its regulatory points and the potential impact of genetic variations.
Mutations in the OTC gene can lead to ornithine transcarbamylase deficiency a disorder affecting ammonia detoxification. This X-linked genetic condition disrupts normal nitrogen metabolism causing ammonia accumulation particularly harmful to the central nervous system. OTC deficiency shares a functional relationship with other urea cycle disorders such as citrullinemia due to its contribution to the same biochemical pathway. Researchers focus on identifying OTC protein mutations to improve diagnosis and treatment of these conditions.
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All lanes: Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [CL4049] (ab262866) at 1 µg/mL
All lanes: Human liver lysate
Predicted band size: 39 kDa
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