Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (AB203859)

Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on Human liver cancer tissue is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (AB203859)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

No staining on Human kidney is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (AB203859)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasmic staining on Human liver tissue is observed [PMID : 17893704].

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (AB203859)

Ornithine Carbamoyltransferase/OTC was immunoprecipitated from 0.35 mg of Human fetal liver lysate with ab203859 at 1/40 dilution.

Western blot was performed from the immunoprecipitate using ab203859 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Human fetal liver lysate, 10μg (Input).

Lane 2 : ab203859 IP in Human fetal liver lysate.

Lane 3 : Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab203859 in Human fetal liver lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

All lanes:

Immunoprecipitation - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859)

Predicted band size: 39 kDa

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• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (AB203859)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized Mouse liver tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green).

Cytoplasmic staining on mouse liver is observed.

Nuclear counterstain : DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (AB203859)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized Mouse kidney tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green).

Weakly staining on mouse kidney.

Nuclear counterstain : DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

• WB

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Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (AB203859)

Blocking/Dilution buffer : 5% NFDM/TBST.

Lane 1:

Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859) at 1/5000 dilution

Lane 2:

Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 20 µg

Lane 2:

Human small intestine lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 40 kDa

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Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (AB203859)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1 : 15 seconds; Lane 2 : 30 seconds.

All lanes:

Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (ab203859) at 1/1000 dilution

Lane 1:

Mouse liver lysate at 20 µg

Lane 2:

Rat liver lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 39 kDa

Observed band size: 40 kDa

false

• WB

CiteAb

Western blot - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] (AB203859)

Ornithine Carbamoyltransferase/OTC western blot using anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] ab203859. Publication image and figure legend from Chow, J. P. H., Cai, Y., et al., 2020, PLoS One, PubMed 32353864.

ab203859 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab203859 please see the product overview.

Expression of ASS1 in various cell lines and the effect of NEI-01 on their cell viability.(A) Expression of ASS1, ASL and OTC in SMMC7721, MIA PaCa-2 and PANC-1. Unperturbed asynchronously growing cells were harvested by trypsinization. After cell lysis, total cell lysates were subject to immunoblot with anti-ASS1, anti-ASL, anti-OTC or anti-ACTIN antibodies. (B) Inhibition of cell growth of MIA PaCa-2 and PANC-1 cells but not SMMC7721 cells by NEI-01 treatment. SMMC7721 cells, MIA PaCa-2 cells or PANC-1 were seeded in 96 well-plate with ~3000 cells/well density 24 hr prior to NEI-01 treatments. After 72 hr, cells were subject to MTT assay. The relative cell viability in % vs the NEI-01 concentration was shown as mean ± SD of triplicate wells. (C) Activation of autophagic pathway upon NEI-01 treatment. MIA PaCa-2 cells were treated with indicated amount of NEI-01 for 3 or 7 days. The cells were then harvested and subject to immunoblot using designated antibodies.

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