Anti-Ornithine Decarboxylase/ODC antibody (ab97395) is a rabbit polyclonal antibody that is used to detect Ornithine Decarboxylase/ODC in Western Blot, IHC-P. Suitable for Human, Mouse, Rat samples.
- Over 10 publications
- Trusted since 2010
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- Journal of enzyme inhibition and medicinal chemistry 38:309-3182022DMSO-tolerant ornithine decarboxylase (ODC) tandem assay optimised for high-throughput screening.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingu Gordon Park,Suyeon Yellena Kim,C Justin LeePubMed 36451618
- Cell & bioscience 12:1832022mTORC1-c-Myc pathway rewires methionine metabolism for HCC progression through suppressing SIRT4 mediated ADP ribosylation of MAT2A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Zhao,Huizhao Su,Xiaomeng Liu,Hongquan Wang,Yukuan Feng,Yan Wang,Haiqiang Chen,Luo Dai,Shihui Lai,Siqi Xu,Chong Li,Jihui Hao,Bo TangPubMed 36371321
- Cancer gene therapy 29:940-9502021The biguanide polyamine analog verlindamycin promotes differentiation in neuroblastoma via induction of antizyme.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZuzanna Urban-Wójciuk,Amy Graham,Karen Barker,Colin Kwok,Yordan Sbirkov,Louise Howell,James Campbell,Patrick M Woster,Evon Poon,Kevin Petrie,Louis CheslerPubMed 34522028
- Cell 184:4186-4202.e202021Polyamine metabolism is a central determinant of helper T cell lineage fidelity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel J Puleston,Francesc Baixauli,David E Sanin,Joy Edwards-Hicks,Matteo Villa,Agnieszka M Kabat,Marcin M Kamiński,Michal Stanckzak,Hauke J Weiss,Katarzyna M Grzes,Klara Piletic,Cameron S Field,Mauro Corrado,Fabian Haessler,Chao Wang,Yaarub Musa,Lena Schimmelpfennig,Lea Flachsmann,Gerhard Mittler,Nir Yosef,Vijay K Kuchroo,Joerg M Buescher,Stefan Balabanov,Edward J Pearce,Douglas R Green,Erika L PearcePubMed 34216540
- Journal of animal science and biotechnology 12:462021Pre-implantation exogenous progesterone and pregnancy in sheep. II. Effects on fetal-placental development and nutrient transporters in late pregnancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatherine M Halloran,Emily C Hoskins,Claire Stenhouse,Robyn M Moses,Kathrin A Dunlap,M Carey Satterfield,Heewon Seo,Gregory A Johnson,Guoyao Wu,Fuller W BazerPubMed 33827696
- Molecules (Basel, Switzerland) 26:2021Metformin Inhibits the Urea Cycle and Reduces Putrescine Generation in Colorectal Cancer Cell Lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTao Zhang,Ling Hu,Jia-Feng Tang,Hang Xu,Kuan Tian,Meng-Na Wu,Shi-Ying Huang,Yu-Mei Du,Peng Zhou,Rui-Jin Lu,Shuang He,Jia-Mei Xu,Jian-Jun Si,Jing Li,Di-Long Chen,Jian-Hua RanPubMed 33915902
- Journal of animal science and biotechnology 12:392021Pre-implantation exogenous progesterone and pregnancy in sheep: I. polyamines, nutrient transport, and progestamedins.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmily C Hoskins,Katherine M Halloran,Claire Stenhouse,Robyn M Moses,Kathrin A Dunlap,Michael C Satterfield,Heewon Seo,Gregory A Johnson,Guoyao Wu,Fuller W BazerPubMed 33663606
- International journal of molecular medicine 47:2021Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinying Zhang et. al.PubMed 33537831
- Journal of cellular physiology 236:5646-56632021Regulation of polyamine homeostasis through an antizyme citrullination pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Fang Yang,Chien-Yun Lee,Ju-Yi Hsieh,Yi-Liang Liu,Chi-Li Lin,Guang-Yaw Liu,Hui-Chih HungPubMed 33432662
- Biochimica et biophysica acta. Molecular cell research 1867:1186732020During mitosis ZEB1 "switches" from being a chromatin-bound epithelial gene repressor, to become a microtubule-associated protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesL Fouani,M L H Huang,L Cole,P J Jansson,Z Kovacevic,D R RichardsonPubMed 32057919
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ODC1 aa 150-450. The exact immunogen used to generate this antibody is proprietary information. Database link P11926
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
| Cow | Predicted | Predicted |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.
Alternative names
Ornithine decarboxylase, ODC, ODC1
Recommended products
Anti-Ornithine Decarboxylase/ODC antibody (ab97395) is a rabbit polyclonal antibody that is used to detect Ornithine Decarboxylase/ODC in Western Blot, IHC-P. Suitable for Human, Mouse, Rat samples.
- Over 10 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ODC1 aa 150-450. The exact immunogen used to generate this antibody is proprietary information. Database link P11926
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Ornithine Decarboxylase/ODC antibody (ab97395) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.
What is the molecular weight of Ornithine Decarboxylase/ODC?
Anti-Ornithine Decarboxylase/ODC (ab97395) specifically detects a band for Ornithine Decarboxylase/ODC (UniProt: P11926) at a molecular weight of 51kDa.
Trusted by the scientific community
Anti-Ornithine Decarboxylase/ODC (ab97395) was first used in a scientific publication in 2010 and has been cited over 10 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ornithine Decarboxylase also known as ODC or ODC1 is an enzyme that plays an important role in polyamine biosynthesis. This enzyme catalyzes the decarboxylation of ornithine to form putrescine a precursor for spermidine and spermine. ODC is a small protein with a molecular mass around 48 kDa. It is expressed in various tissues including the liver kidney and brain. ODC expression tends to be highest in rapidly growing tissues due to its involvement in cell growth and differentiation.
- Biological function summary
ODC actively participates in cell proliferation and differentiation by regulating the levels of polyamines which are essential for DNA stabilization and cellular processes. While ODC does not form part of a larger protein complex its activity requires the presence of cofactor pyridoxal 5'-phosphate. By controlling polyamine synthesis ODC influences gene expression ion channel functions and protein synthesis impacting cell cycle progression and apoptosis.
- Pathways
Ornithine decarboxylation connects with important biological processes such as the urea cycle and polyamine biosynthetic pathway. In these pathways enzymes like arginase and S-adenosylmethionine decarboxylase work closely with ODC. ODC's role in regulating polyamine levels makes it an important component of cellular metabolism and growth pathways. Through these pathways ODC coordinates with other proteins to ensure proper cellular function and response to growth signals.
- Associated diseases and disorders
ODC activity has been linked to cancer and neurological disorders. Overexpression and dysregulation of ODC can lead to increased polyamine levels which associate with tumor development and proliferation particularly in colorectal cancer. ODC also connects with proteins like c-Myc which regulates its expression and is often dysregulated in cancer. In neurological disorders abnormal ODC function affects neurodegeneration influencing proteins involved in neuronal survival and plasticity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-Ornithine Decarboxylase/ODC antibody (ab97395)
The signal was developed with Trident ECL plus-Enhanced.
All lanes: Western blot - Anti-Ornithine Decarboxylase/ODC antibody (ab97395) at 1/1000 dilution
Lane 1: HepG2 whole cell lysate at 30 µg
Lane 2: T47D whole cell lysate at 30 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG antibody (HRP)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody (ab97395)
Paraffin embedded rat adrenal gland tissue stained for ODC using ab97395 at 1/500 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody (ab97395)
Paraffin embedded mouse heart tissue stained for ODC using ab97395 at 1/1000 dilution in immunohistochemical analysis.
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