AB97395

Anti-Ornithine Decarboxylase/ODC antibody

Name: Anti-Ornithine Decarboxylase/ODC antibody (ab97395) | Abcam
Brand: Abcam Limited
SKU: AB97395
Price: 430 USD
Availability: InStock

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(18 Publications)

Anti-Ornithine Decarboxylase/ODC antibody (ab97395) is a rabbit polyclonal antibody detecting Ornithine Decarboxylase/ODC in Western Blot, IHC-P. Suitable for Human, Mouse, Rat.

- Over 10 publications
- Trusted since 2010

View Alternative Names

Ornithine decarboxylase, ODC, ODC1

3 Images

Supplier Data

Western blot - Anti-Ornithine Decarboxylase/ODC antibody (AB97395)

The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-Ornithine Decarboxylase/ODC antibody (ab97395) at 1/1000 dilution

Lane 1:

HepG2 whole cell lysate at 30 µg

Lane 2:

T47D whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG antibody (HRP)

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody (AB97395)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody (AB97395)

Paraffin embedded mouse heart tissue stained for ODC using ab97395 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody (AB97395)

Paraffin embedded rat adrenal gland tissue stained for ODC using ab97395 at 1/500 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human ODC1 aa 150-450. The exact immunogen used to generate this antibody is proprietary information.

P11926

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Ornithine Decarboxylase/ODC antibody (ab97395) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse, Rat samples.

What is the molecular weight of Ornithine Decarboxylase/ODC?
Anti-Ornithine Decarboxylase/ODC (ab97395) specifically detects a band for Ornithine Decarboxylase/ODC (UniProt: P11926) at a molecular weight of 51kDa.

Trusted by the scientific community
Anti-Ornithine Decarboxylase/ODC (ab97395) was first used in a scientific publication in 2010 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ornithine Decarboxylase also known as ODC or ODC1 is an enzyme that plays an important role in polyamine biosynthesis. This enzyme catalyzes the decarboxylation of ornithine to form putrescine a precursor for spermidine and spermine. ODC is a small protein with a molecular mass around 48 kDa. It is expressed in various tissues including the liver kidney and brain. ODC expression tends to be highest in rapidly growing tissues due to its involvement in cell growth and differentiation.

Biological function summary

ODC actively participates in cell proliferation and differentiation by regulating the levels of polyamines which are essential for DNA stabilization and cellular processes. While ODC does not form part of a larger protein complex its activity requires the presence of cofactor pyridoxal 5'-phosphate. By controlling polyamine synthesis ODC influences gene expression ion channel functions and protein synthesis impacting cell cycle progression and apoptosis.

Pathways

Ornithine decarboxylation connects with important biological processes such as the urea cycle and polyamine biosynthetic pathway. In these pathways enzymes like arginase and S-adenosylmethionine decarboxylase work closely with ODC. ODC's role in regulating polyamine levels makes it an important component of cellular metabolism and growth pathways. Through these pathways ODC coordinates with other proteins to ensure proper cellular function and response to growth signals.

ODC activity has been linked to cancer and neurological disorders. Overexpression and dysregulation of ODC can lead to increased polyamine levels which associate with tumor development and proliferation particularly in colorectal cancer. ODC also connects with proteins like c-Myc which regulates its expression and is often dysregulated in cancer. In neurological disorders abnormal ODC function affects neurodegeneration influencing proteins involved in neuronal survival and plasticity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.

See full target information ODC1

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Infection and immunity 91:e0023623 PubMed37861311

2023

Polyamines and hypusination are important for toxin B (TcdB)-mediated activation of group 3 innate lymphocytes (ILC3s).

Applications

Unspecified application

Species

Unspecified reactive species

Prakash Sah,Bailey A Knighten,Megan A Reidy,Lauren A Zenewicz

ImmunoHorizons 7:41-48 PubMed36637514

2023

The Polyamine Putrescine Is a Positive Regulator of Group 3 Innate Lymphocyte Activation.

Applications

Unspecified application

Species

Unspecified reactive species

Prakash Sah,Lauren A Zenewicz

Journal of enzyme inhibition and medicinal chemistry 38:309-318 PubMed36451618

2022

DMSO-tolerant ornithine decarboxylase (ODC) tandem assay optimised for high-throughput screening.

Applications

Unspecified application

Species

Unspecified reactive species

Mingu Gordon Park,Suyeon Yellena Kim,C Justin Lee

Cell & bioscience 12:183 PubMed36371321

2022

mTORC1-c-Myc pathway rewires methionine metabolism for HCC progression through suppressing SIRT4 mediated ADP ribosylation of MAT2A.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Zhao,Huizhao Su,Xiaomeng Liu,Hongquan Wang,Yukuan Feng,Yan Wang,Haiqiang Chen,Luo Dai,Shihui Lai,Siqi Xu,Chong Li,Jihui Hao,Bo Tang

Cancer gene therapy 29:940-950 PubMed34522028

2021

The biguanide polyamine analog verlindamycin promotes differentiation in neuroblastoma via induction of antizyme.

Applications

Unspecified application

Species

Unspecified reactive species

Zuzanna Urban-Wójciuk,Amy Graham,Karen Barker,Colin Kwok,Yordan Sbirkov,Louise Howell,James Campbell,Patrick M Woster,Evon Poon,Kevin Petrie,Louis Chesler

Cell 184:4186-4202.e20 PubMed34216540

2021

Polyamine metabolism is a central determinant of helper T cell lineage fidelity.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel J Puleston,Francesc Baixauli,David E Sanin,Joy Edwards-Hicks,Matteo Villa,Agnieszka M Kabat,Marcin M Kamiński,Michal Stanckzak,Hauke J Weiss,Katarzyna M Grzes,Klara Piletic,Cameron S Field,Mauro Corrado,Fabian Haessler,Chao Wang,Yaarub Musa,Lena Schimmelpfennig,Lea Flachsmann,Gerhard Mittler,Nir Yosef,Vijay K Kuchroo,Joerg M Buescher,Stefan Balabanov,Edward J Pearce,Douglas R Green,Erika L Pearce

Journal of animal science and biotechnology 12:46 PubMed33827696

2021

Pre-implantation exogenous progesterone and pregnancy in sheep. II. Effects on fetal-placental development and nutrient transporters in late pregnancy.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine M Halloran,Emily C Hoskins,Claire Stenhouse,Robyn M Moses,Kathrin A Dunlap,M Carey Satterfield,Heewon Seo,Gregory A Johnson,Guoyao Wu,Fuller W Bazer

Molecules (Basel, Switzerland) 26: PubMed33915902

2021

Metformin Inhibits the Urea Cycle and Reduces Putrescine Generation in Colorectal Cancer Cell Lines.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Zhang,Ling Hu,Jia-Feng Tang,Hang Xu,Kuan Tian,Meng-Na Wu,Shi-Ying Huang,Yu-Mei Du,Peng Zhou,Rui-Jin Lu,Shuang He,Jia-Mei Xu,Jian-Jun Si,Jing Li,Di-Long Chen,Jian-Hua Ran

Journal of animal science and biotechnology 12:39 PubMed33663606

2021

Pre-implantation exogenous progesterone and pregnancy in sheep: I. polyamines, nutrient transport, and progestamedins.

Applications

Unspecified application

Species

Unspecified reactive species

Emily C Hoskins,Katherine M Halloran,Claire Stenhouse,Robyn M Moses,Kathrin A Dunlap,Michael C Satterfield,Heewon Seo,Gregory A Johnson,Guoyao Wu,Fuller W Bazer

International journal of molecular medicine 47: PubMed33537831

2021

Exogenous spermine attenuates diabetic kidney injury in rats by inhibiting AMPK/mTOR signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xinying Zhang,Li Zhang,Zhe Chen,Siwei Li,Bingbing Che,Ningning Wang,Junting Chen,Changqing Xu,Can Wei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ornithine Decarboxylase/ODC antibody

Western blot - Anti-Ornithine Decarboxylase/ODC antibody (AB97395)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody (AB97395)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody (AB97395)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise