Anti-Ornithine Decarboxylase/ODC antibody [EPR29794-513]

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Ornithine Decarboxylase/ODC antibody [EPR29794-513] (AB324979)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml LPS for 24hours (Lower left and right) and Untreated RAW 264.7 (Upper left and right) cells labelling Ornithine Decarboxylase/ODC with ab324979 at 1/50 dilution (1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ornithine Decarboxylase/ODC antibody [EPR29794-513] (AB324979)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) and RAW 264.7 treated with lipopolysaccharide (100 ng/mL) for 24 hr labelling Ornithine Decarboxylase/ODC with ab324979 at 1/200 (2.48 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing increased cytoplasmic staining in RAW 264.7 cell line (shown in green) treated with lipopolysaccharide (100 ng/mL) for 24 hr. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

• WB

Lab

Western blot - Anti-Ornithine Decarboxylase/ODC antibody [EPR29794-513] (AB324979)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : brain.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lanes 1-2 : 26 seconds, lanes 3-4 : 180 seconds.

All lanes:

Western blot - Anti-Ornithine Decarboxylase/ODC antibody [EPR29794-513] (ab324979) at 1/1000 dilution

Lane 1:

Mouse kidney tissue lysate at 40 µg

Lane 2:

Mouse brain tissue lysate at 40 µg

Lane 3:

Untreated RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 50 µg

Lane 4:

RAW 264.7 treated with 100ng/ml LPS for 24 hours whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa,36 kDa

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