Rabbit Recombinant Monoclonal Ornithine Decarboxylase/ODC antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ODC1.
Images
![Western blot - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] - BSA and Azide free (AB270300), expandable thumbnail](https://content.abcam.com/products/images/ornithine-decarboxylase-odc-antibody-odc1-3636r-bsa-and-azide-free-ab270300--western-blot-img20666.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] - BSA and Azide free (AB270300)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] - BSA and Azide free (AB270300), expandable thumbnail](https://content.abcam.com/products/images/ornithine-decarboxylase-odc-antibody-odc1-3636r-bsa-and-azide-free-ab270300--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img47265.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] - BSA and Azide free (AB270300)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human ODC1. Database link P11926
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.5-1 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25-0.5 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.
Alternative names
Ornithine decarboxylase, ODC, ODC1
Recommended products
Rabbit Recombinant Monoclonal Ornithine Decarboxylase/ODC antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ODC1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human ODC1. Database link P11926
- Carrier free
- Yes
- Clone number
- ODC1/3636R
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab270300 is a carrier free version of Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] ab270269.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ornithine Decarboxylase also known as ODC or ODC1 is an enzyme that plays an important role in polyamine biosynthesis. This enzyme catalyzes the decarboxylation of ornithine to form putrescine a precursor for spermidine and spermine. ODC is a small protein with a molecular mass around 48 kDa. It is expressed in various tissues including the liver kidney and brain. ODC expression tends to be highest in rapidly growing tissues due to its involvement in cell growth and differentiation.
- Biological function summary
ODC actively participates in cell proliferation and differentiation by regulating the levels of polyamines which are essential for DNA stabilization and cellular processes. While ODC does not form part of a larger protein complex its activity requires the presence of cofactor pyridoxal 5'-phosphate. By controlling polyamine synthesis ODC influences gene expression ion channel functions and protein synthesis impacting cell cycle progression and apoptosis.
- Pathways
Ornithine decarboxylation connects with important biological processes such as the urea cycle and polyamine biosynthetic pathway. In these pathways enzymes like arginase and S-adenosylmethionine decarboxylase work closely with ODC. ODC's role in regulating polyamine levels makes it an important component of cellular metabolism and growth pathways. Through these pathways ODC coordinates with other proteins to ensure proper cellular function and response to growth signals.
- Associated diseases and disorders
ODC activity has been linked to cancer and neurological disorders. Overexpression and dysregulation of ODC can lead to increased polyamine levels which associate with tumor development and proliferation particularly in colorectal cancer. ODC also connects with proteins like c-Myc which regulates its expression and is often dysregulated in cancer. In neurological disorders abnormal ODC function affects neurodegeneration influencing proteins involved in neuronal survival and plasticity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] - BSA and Azide free (ab270300), expandable thumbnail](https://content.abcam.com/products/images/ornithine-decarboxylase-odc-antibody-odc1-3636r-bsa-and-azide-free-ab270300--western-blot-img20666.jpg "Western blot - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] - BSA and Azide free (ab270300)")
Western blot - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] - BSA and Azide free (ab270300)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] ab270269).
All lanes: Western blot - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] - BSA and Azide free (ab270300) at 2 µg/mL
All lanes: PC3 (Human prostate adenocarcinoma cell line) cell lysate.
Predicted band size: 51 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] - BSA and Azide free (ab270300), expandable thumbnail](https://content.abcam.com/products/images/ornithine-decarboxylase-odc-antibody-odc1-3636r-bsa-and-azide-free-ab270300--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img47265.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] - BSA and Azide free (ab270300)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] - BSA and Azide free (ab270300)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Ornithine Decarboxylase/ODC using Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] ab270269 at 0.5 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (Anti-Ornithine Decarboxylase/ODC antibody [ODC1/3636R] ab270269).
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