AB220205

Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487]

Name: Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (ab220205) | Abcam
Brand: Abcam Limited
SKU: AB220205

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Mouse Monoclonal Ornithine Decarboxylase/ODC antibody. Suitable for IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ODC1.

View Alternative Names

Ornithine decarboxylase, ODC, ODC1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (AB220205)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (AB220205)

Immunofluorescence staining of LNCap (Human prostate cancer cell line) cells labeling Ornithine Decarboxylase/ODC (Green) using ab220205 at 2 μg/ml. The counterstain was DAPI (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (AB220205)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (AB220205)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat pancreas tissue labeling Ornithine Decarboxylase with ab220205 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (AB220205)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse small intestine tissue labeling Ornithine Decarboxylase with ab220205 at 1 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (AB220205)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse pancreas tissue labeling Ornithine Decarboxylase with ab220205 at 1 μg/ml.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

ODC1/487

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ODC1.

P11926

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 minutes at RT). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "(Primary incubation for 30 minutes at RT). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab220205 was purified from Bioreactor Concentrate by Protein A/G.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ornithine Decarboxylase also known as ODC or ODC1 is an enzyme that plays an important role in polyamine biosynthesis. This enzyme catalyzes the decarboxylation of ornithine to form putrescine a precursor for spermidine and spermine. ODC is a small protein with a molecular mass around 48 kDa. It is expressed in various tissues including the liver kidney and brain. ODC expression tends to be highest in rapidly growing tissues due to its involvement in cell growth and differentiation.

Biological function summary

ODC actively participates in cell proliferation and differentiation by regulating the levels of polyamines which are essential for DNA stabilization and cellular processes. While ODC does not form part of a larger protein complex its activity requires the presence of cofactor pyridoxal 5'-phosphate. By controlling polyamine synthesis ODC influences gene expression ion channel functions and protein synthesis impacting cell cycle progression and apoptosis.

Pathways

Ornithine decarboxylation connects with important biological processes such as the urea cycle and polyamine biosynthetic pathway. In these pathways enzymes like arginase and S-adenosylmethionine decarboxylase work closely with ODC. ODC's role in regulating polyamine levels makes it an important component of cellular metabolism and growth pathways. Through these pathways ODC coordinates with other proteins to ensure proper cellular function and response to growth signals.

ODC activity has been linked to cancer and neurological disorders. Overexpression and dysregulation of ODC can lead to increased polyamine levels which associate with tumor development and proliferation particularly in colorectal cancer. ODC also connects with proteins like c-Myc which regulates its expression and is often dysregulated in cancer. In neurological disorders abnormal ODC function affects neurodegeneration influencing proteins involved in neuronal survival and plasticity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first and rate-limiting step of polyamine biosynthesis that converts ornithine into putrescine, which is the precursor for the polyamines, spermidine and spermine. Polyamines are essential for cell proliferation and are implicated in cellular processes, ranging from DNA replication to apoptosis.

See full target information ODC1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487]

Immunocytochemistry/ Immunofluorescence - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (AB220205)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (AB220205)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (AB220205)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Decarboxylase/ODC antibody [ODC1/487] (AB220205)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise