AB322041

Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6]

Recombinant
RabMAb
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Rabbit Recombinant Monoclonal Orosomucoid 2 antibody. Suitable for Dot, WB and reacts with Recombinant full length protein - Human, Recombinant fragment - Human, Human samples.

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Supplier Data

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (AB322041)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed Mw of ~45KDa is higher than the predicted Mw due to glycosylation (PMID : 33070381).

All lanes:

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (ab322041) at 1/1000 dilution

All lanes:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 45 kDa

false

Exposure time: 114s

Supplier Data

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (AB322041)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, skeletal muscle(PMID : 20442402)

The observed Mw of ~45KDa is higher than the predicted Mw due to glycosylation (PMID : 33070381).

The identity of the lower MW band at approximately 18 kDa (in lane 1) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (ab322041) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Lane 4:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 45 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Dot Blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (AB322041)

Dot blot analysis of Orosomucoid 1 + Orosomucoid 2 using ab322041 at 1 : 1000 (0.508 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 10,000 dilution.

Lane1 : His-tagged human ORM1 protein
Lane2 : His-tagged human ORM2 protein

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (ab322041) at 1/1000 dilution

Lane 1:

His-tagged human ORM1 protein at 5 ng

Lane 2:

His-tagged human ORM2 protein at 5 ng

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

false

Exposure time: 180s

Carrier free

Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26603-6

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Alpha 1 Acid Glycoprotein also known as AGP or Orosomucoid 2 is a plasma protein with a molecular mass of around 40-45 kDa. This glycoprotein is rich in carbohydrate content with about 45% of its mass made up of glycan chains. It is primarily synthesized in the liver and circulates in the bloodstream. AGP functions as a major acute-phase protein meaning its levels increase in response to inflammation or tissue injury.

Biological function summary

AGP has significant roles in modulating immune responses and transport functions. This molecule interacts with various drugs and hormones due to its binding capacity thereby influencing their bioavailability and distribution. It forms part of a complex dynamic system that helps modulate the body's response to acute inflammatory stimuli. As a glycoprotein AGP plays a role in processes such as cell-to-cell communication and cell signaling which are essential for maintaining homeostasis during stress responses.

Pathways

AGP is integrally involved in the acute-phase response pathway that gets activated during infection or injury. This pathway leads to the dramatic increase of AGP and other acute-phase proteins like C-reactive protein (CRP) in the blood. Furthermore AGP is related to the immune response pathways where it can modulate the activity of various immune cells. Its binding interactions with cytokines and other inflammatory modulators emphasize its role in regulating inflammation and immune responses.

AGP levels often correlate with conditions involving acute and chronic inflammation like rheumatoid arthritis and cancer. Elevated AGP levels are frequently observed in patients with these disorders and reflect the body's ongoing inflammatory response. The increase of AGP in these diseases often correlates with other proteins such as serum amyloid A (SAA) and CRP which serve similar functions in the acute-phase response. This correlation with other inflammatory markers highlights AGP's importance in understanding and potentially diagnosing inflammatory conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction.

See full target information ORM2

Additional targets

ORM1

Alternative Names

AGP2, ORM2, Alpha-1-acid glycoprotein 2, AGP 2, Orosomucoid-2, OMD 2, AGP1, ORM1, Alpha-1-acid glycoprotein 1, AGP 1, Orosomucoid-1, OMD 1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6]

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (AB322041)

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (AB322041)

Dot Blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (AB322041)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Assay Kits

Proteins & Peptides

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Alternative Names

Product promise