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AB322042

Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] - BSA and Azide free

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Rabbit Recombinant Monoclonal Orosomucoid 2 antibody. Carrier free. Suitable for Dot, WB and reacts with Recombinant full length protein - Human, Recombinant fragment - Human, Human samples.

View Alternative Names

AGP2, ORM2, Alpha-1-acid glycoprotein 2, AGP 2, Orosomucoid-2, OMD 2

3 Images
Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] - BSA and Azide free (AB322042)
  • WB

Supplier Data

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] - BSA and Azide free (AB322042)

This data was developed using ab322041, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed Mw of ~45KDa is higher than the predicted Mw due to glycosylation (PMID : 33070381).

All lanes:

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (<a href='/en-us/products/primary-antibodies/orosomucoid-1-orosomucoid-2-antibody-epr26603-6-ab322041'>ab322041</a>) at 1/1000 dilution

All lanes:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 45 kDa

false

Exposure time: 114s

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] - BSA and Azide free (AB322042)
  • WB

Supplier Data

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] - BSA and Azide free (AB322042)

This data was developed using ab322041, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen skeletal muscle(PMID : 20442402)

The observed Mw of ~45KDa is higher than the predicted Mw due to glycosylation (PMID : 33070381).

The identity of the lower MW band at approximately 18 kDa (in lane 1) is unknown.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (<a href='/en-us/products/primary-antibodies/orosomucoid-1-orosomucoid-2-antibody-epr26603-6-ab322041'>ab322041</a>) at 1/1000 dilution

Lane 1:

Human plasma at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Human skeletal muscle tissue lysate at 20 µg

Lane 4:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 45 kDa,36 kDa

false

Exposure time: 180s

Dot Blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] - BSA and Azide free (AB322042)
  • Dot

Supplier Data

Dot Blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] - BSA and Azide free (AB322042)

This data was developed using ab322041, the same antibody clone in a different buffer formulation.

Dot blot analysis of Orosomucoid 1 + Orosomucoid 2 using ab322041 at 1 : 1000 (0.508 ug/ml) followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1 : 10000 dilution.

Lane1 : His-tagged human ORM1 protein
Lane2 : His-tagged human ORM2 protein

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6] (<a href='/en-us/products/primary-antibodies/orosomucoid-1-orosomucoid-2-antibody-epr26603-6-ab322041'>ab322041</a>) at 1/1000 dilution

Lane 1:

His-tagged human ORM1 protein at 5 ng

Lane 2:

His-tagged human ORM2 protein at 5 ng

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/10000 dilution

false

Exposure time: 180s

  • Unconjugated

    Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-6]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26603-6

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Dot, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab322042 is the carrier-free version of ab322041.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction.
See full target information ORM2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com