Skip to main content

Rabbit Recombinant Monoclonal Orosomucoid 2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Anti-Orosomucoid 1 + Orosomucoid 2 antibody [EPR26603-87] - BSA and Azide free (Capture) (AB315499), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
sELISA
Human
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Functions as a transport protein in the blood stream. Binds various hydrophobic ligands in the interior of its beta-barrel domain. Also binds synthetic drugs and influences their distribution and availability. Appears to function in modulating the activity of the immune system during the acute-phase reaction.

Additional Targets

ORM1

Alternative names

AGP2, ORM2, Alpha-1-acid glycoprotein 2, AGP 2, Orosomucoid-2, OMD 2

Recommended products

Rabbit Recombinant Monoclonal Orosomucoid 2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26603-87
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab315499 is a BSA and Azide free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Orosomucoid 2. The recommended pair for sandwich ELISA is:

Capture: ab315499, Human Orosomucoid 2 Capture Antibody (unconjugated)

Detector: Anti-Orosomucoid 2 antibody [EPR26603-23] - BSA and Azide free (Detector) ab315500, Human Orosomucoid 2 Detector Antibody (unconjugated)

The reference range value is 62.5-4000 pg/mL.

The recommended antibody orientation is based on internal optimization for ELISAbased assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Alpha 1 Acid Glycoprotein also known as AGP or Orosomucoid has a molecular mass of about 41-43 kDa. This glycoprotein mainly occurs in the liver and circulates in the bloodstream. It belongs to the acute-phase protein family and increases in response to inflammation and infection. Its carbohydrate-rich composition contributes to its role in various physiological processes.

Biological function summary

The glycoprotein acts as a transport protein modulating the distribution and availability of various endogenous and exogenous substances including pharmaceuticals. It does not form part of a larger complex but influences the pharmacokinetics of drugs by binding to them and altering their free concentration. It shows anti-inflammatory properties by inhibiting immune cell activity and affecting cytokine production.

Pathways

AGP interacts in the acute-phase response pathway which is essential for the immediate response to infection or injury. It connects with several inflammatory mediators including cytokines like IL-6. Additionally it relates to the drug metabolism pathway impacting the transportation and efficacy of drugs while proteins such as albumin and transthyretin also play roles in these processes.

Associated diseases and disorders

Elevated levels of AGP associate with inflammatory conditions including rheumatoid arthritis and cancer. In rheumatoid arthritis inflammation causes overproduction of this protein influencing drug distribution. In cancer its levels correlate with tumor progression and metastasis. AGP interacts with other acute-phase proteins like C-reactive protein which increases under similar pathological conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com