AB194957

Anti-ORP-11 antibody - N-terminal

Name: Anti-ORP-11 antibody - N-terminal (ab194957) | Abcam
Brand: Abcam Limited
SKU: AB194957
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal ORP-11 antibody. N-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human OSBPL11 aa 1-50.

View Alternative Names

ORP11, OSBP12, OSBPL11, Oxysterol-binding protein-related protein 11, ORP-11, OSBP-related protein 11

2 Images

Supplier Data

Immunoprecipitation - Anti-ORP-11 antibody - N-terminal (AB194957)

Detection of ORP-11 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab194957 at 6 μg/mg lysate for IP and at 0.1 μg/ml for subsequent Western blot detection.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ORP-11 antibody - N-terminal (ab194957)

Predicted band size: 84 kDa

false

Supplier Data

Western blot - Anti-ORP-11 antibody - N-terminal (AB194957)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-ORP-11 antibody - N-terminal (ab194957) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 84 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human OSBPL11 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9BXB4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Cat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Common marmoset": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab194957 was affinity purified using an epitope specific to ORP-11 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ORP-11 also known as oxysterol-binding protein-related protein 11 is a lipid-binding protein involved in intracellular lipid transport. It has a mass of approximately 52 kDa. ORP-11 is expressed in various tissues with notable expression in the brain as well as in the liver. It functions at cellular membranes where it facilitates the transfer and regulation of specific lipids maintaining cellular homeostasis.

Biological function summary

Oxysterol-binding protein-related protein 11 is part of a broader family which plays roles in lipid sensing and signaling. It potentially interacts with and forms complexes with other member proteins within this family allowing for coordination in lipid transport and metabolism. ORP-11 is essential in maintaining proper lipid balance in cells influencing signaling pathways that depend on lipid molecules as messengers.

Pathways

ORP-11 participates in the PI3K/Akt signaling pathway which is important for cell survival and growth. It also fits into lipid metabolism pathways where it interacts with proteins like PI4P and other OSBP family members. These interactions are important for the regulation of phosphoinositides affecting the cell's membrane dynamics and intracellular signaling processes.

ORP-11 has been linked to metabolic disorders including non-alcoholic fatty liver disease (NAFLD). Disruptions in ORP-11 function may result in improper lipid metabolism contributing to the pathogenesis of this liver disorder. Additionally it is associated with certain neurological conditions as its expression in the brain suggests it may interact with proteins like ApoE which plays a role in neurodegenerative diseases. ORP-11's involvement in lipid regulation pathways highlights its importance in maintaining cellular function and its potential connection to disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in regulating ADIPOQ and FABP4 levels in differentiating adipocytes and is also involved in regulation of adipocyte triglyceride storage (PubMed : 23028956). Weakly binds 25-hydroxycholesterol (PubMed : 17428193).

See full target information OSBPL11

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:33-43 PubMed36300343

2022

AKBA inhibits radiotherapy resistance in lung cancer by inhibiting maspin methylation and regulating the AKT/FOXO1/p21 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chun Gong,Wei Li,Jing Wu,Yao-Yao Li,Yi Ma,Li-Wen Tang

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ORP-11 antibody - N-terminal

Immunoprecipitation - Anti-ORP-11 antibody - N-terminal (AB194957)

Western blot - Anti-ORP-11 antibody - N-terminal (AB194957)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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