AB192998

Anti-ORP-3 antibody

Name: Anti-ORP-3 antibody (ab192998) | Abcam
Brand: Abcam Limited
SKU: AB192998
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal ORP-3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human OSBPL3 aa 450-550.

View Alternative Names

KIAA0704, ORP3, OSBP3, OSBPL3, Oxysterol-binding protein-related protein 3, ORP-3, OSBP-related protein 3

2 Images

Supplier Data

Immunoprecipitation - Anti-ORP-3 antibody (AB192998)

Detection of ORP-3 in immunoprecipitates of 293T whole cell lysate (prepared using NETN lysis buffer; 1 mg for IP, 20% of IP loaded) using ab192998 at 6 μg/mg lysate for IP and at 1 μg/ml for subsequent Western blot detection (lane 1). Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-ORP-3 antibody (ab192998)

Predicted band size: 101 kDa

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Supplier Data

Western blot - Anti-ORP-3 antibody (AB192998)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-ORP-3 antibody (ab192998) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 101 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human OSBPL3 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

Q9H4L5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab192998 was affinity purified using an epitope specific to ORP-3 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ORP-3 also known as Oxysterol-binding protein-related protein 3 is a member of the oxysterol-binding protein family. It has a mass of approximately 100 kDa and is broadly expressed in a variety of tissues including the liver and spleen. ORP-3 contains a pleckstrin homology domain and an oxysterol-binding domain enabling its interactions with lipids and membranes. These structural aspects allow ORP-3 to have a role in lipid transport and cellular signaling.

Biological function summary

The protein contributes significantly to the regulation of lipid metabolism and cellular homeostasis. It is part of a complex that modulates lipid transport and signaling pathways affecting cellular growth and proliferation. ORP-3's interaction with phosphoinositides is particularly important for maintaining membrane integrity and function. These activities can influence cell movement and positioning which have implications in cellular responses to external stimuli.

Pathways

The protein interacts with several key pathways including the phosphoinositide signaling pathway and the sterol regulatory element-binding protein (SREBP) pathway. In the phosphoinositide pathway ORP-3 interacts with proteins like phosphatidylinositol 4-phosphate to facilitate lipid transport processes. In the SREBP pathway it indirectly influences cholesterol and fatty acid metabolism. These pathways position ORP-3 as an integral component in lipid homeostasis and signaling networks.

Disruptions in ORP-3 function link to lipid-related diseases such as atherosclerosis and certain cancers. In atherosclerosis ORP-3 contributes to cholesterol metabolism potentially affecting plaque formation. Its role in cancers involves its impact on cell proliferation and migration with connections to proteins such as Akt involved in cellular survival pathways. Understanding these associations helps in targeting ORP-3 for therapeutic interventions in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes (PubMed : 16143324). Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments (PubMed : 25447204). The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed : 18270267, PubMed : 25447204). With VAPA, may regulate ER morphology (PubMed : 16143324). Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion (PubMed : 18270267). Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3 (PubMed : 16143324). Also binds 25-hydroxycholesterol and cholesterol (PubMed : 17428193).

See full target information OSBPL3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 43:29 PubMed38263248

2024

HIF-2α/LINC02609/APOL1-mediated lipid storage promotes endoplasmic reticulum homeostasis and regulates tumor progression in clear-cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Haibing Xiao,Yan Qu,Haolin Li,Yi Zhang,Mintian Fei,Chaozhao Liang,Hongmei Yang,Xiaoping Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ORP-3 antibody

Immunoprecipitation - Anti-ORP-3 antibody (AB192998)

Western blot - Anti-ORP-3 antibody (AB192998)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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