AB224212

Anti-ORP-3 antibody

Name: Anti-ORP-3 antibody (ab224212) | Abcam
Brand: Abcam Limited
SKU: AB224212

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(1 Publication)

Rabbit Polyclonal ORP-3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human OSBPL3 aa 300-450.

View Alternative Names

KIAA0704, ORP3, OSBP3, OSBPL3, Oxysterol-binding protein-related protein 3, ORP-3, OSBP-related protein 3

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-ORP-3 antibody (AB224212)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ORP-3 antibody (AB224212)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for ORP-3 (green) using ab224212 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP-3 antibody (AB224212)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP-3 antibody (AB224212)

Paraffin-embedded human urinary bladder tissue stained for ORP-3 using ab224212 at 1/50 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-ORP-3 antibody (AB224212)

All lanes:

Western blot - Anti-ORP-3 antibody (ab224212) at 1/250 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 101 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human OSBPL3 aa 300-450. The exact immunogen used to generate this antibody is proprietary information.

Q9H4L5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ORP-3 also known as Oxysterol-binding protein-related protein 3 is a member of the oxysterol-binding protein family. It has a mass of approximately 100 kDa and is broadly expressed in a variety of tissues including the liver and spleen. ORP-3 contains a pleckstrin homology domain and an oxysterol-binding domain enabling its interactions with lipids and membranes. These structural aspects allow ORP-3 to have a role in lipid transport and cellular signaling.

Biological function summary

The protein contributes significantly to the regulation of lipid metabolism and cellular homeostasis. It is part of a complex that modulates lipid transport and signaling pathways affecting cellular growth and proliferation. ORP-3's interaction with phosphoinositides is particularly important for maintaining membrane integrity and function. These activities can influence cell movement and positioning which have implications in cellular responses to external stimuli.

Pathways

The protein interacts with several key pathways including the phosphoinositide signaling pathway and the sterol regulatory element-binding protein (SREBP) pathway. In the phosphoinositide pathway ORP-3 interacts with proteins like phosphatidylinositol 4-phosphate to facilitate lipid transport processes. In the SREBP pathway it indirectly influences cholesterol and fatty acid metabolism. These pathways position ORP-3 as an integral component in lipid homeostasis and signaling networks.

Disruptions in ORP-3 function link to lipid-related diseases such as atherosclerosis and certain cancers. In atherosclerosis ORP-3 contributes to cholesterol metabolism potentially affecting plaque formation. Its role in cancers involves its impact on cell proliferation and migration with connections to proteins such as Akt involved in cellular survival pathways. Understanding these associations helps in targeting ORP-3 for therapeutic interventions in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes (PubMed : 16143324). Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments (PubMed : 25447204). The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface (PubMed : 18270267, PubMed : 25447204). With VAPA, may regulate ER morphology (PubMed : 16143324). Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion (PubMed : 18270267). Binds to phosphoinositides with preference for PI(3,4)P2 and PI(3,4,5)P3 (PubMed : 16143324). Also binds 25-hydroxycholesterol and cholesterol (PubMed : 17428193).

See full target information OSBPL3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 15:e70464 PubMed40985995

2025

CD103CD8 tissue-resident memory T lymphocytes of melanoma boost anti-tumour immunity and predict immunotherapy outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyi Zhang,Junquan Song,Yinlam Li,Kangjie Shen,Jiangying Xuan,Yuan Gao,Lili Lu,Zhi Pang,Lu Wang,Yang Yang,Zixu Gao,Qianrong Hu,Yu Zhu,Chenlu Wei,Shaoluan Zheng,Rongkui Luo,Yingyong Hou,Yuhong Zhou,Chuanyuan Wei,Jianying Gu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ORP-3 antibody

Immunocytochemistry/ Immunofluorescence - Anti-ORP-3 antibody (AB224212)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP-3 antibody (AB224212)

Western blot - Anti-ORP-3 antibody (AB224212)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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