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AB203352

Anti-ORP1 antibody

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(1 Publication)

Rabbit Polyclonal ORP1 antibody. Suitable for IHC-P, WB and reacts with Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human OSBPL1A aa 900-950 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

ORP1, OSBP8, OSBPL1, OSBPL1B, OSBPL1A, Oxysterol-binding protein-related protein 1, ORP-1, OSBP-related protein 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP1 antibody (AB203352)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP1 antibody (AB203352)

Immunohistochemical analysis of formalin-fixed, paraffin embedded rat testis tissue labeling ORP1 with ab203352 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Western blot - Anti-ORP1 antibody (AB203352)
  • WB

Supplier Data

Western blot - Anti-ORP1 antibody (AB203352)

All lanes:

Western blot - Anti-ORP1 antibody (ab203352) at 1/300 dilution

All lanes:

Mouse brain lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated (90min in 37˚C) at 1/5000 dilution

Predicted band size: 108 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human OSBPL1A aa 900-950 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9BXW6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>(or 1/50 -1/200 using a fluorescent secondary antibody).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ORP1 also known as oxysterol-binding protein-related protein 1 is a member of the OSBP (oxysterol-binding protein) family. It functions as a lipid transfer protein involved in the regulation of intracellular lipid dynamics. ORP1 weighs approximately 108 kDa and is ubiquitously expressed in various tissues with higher levels in the liver and brain. The protein interacts with lipids and plays a role in the control of cholesterol and phosphoinositide levels by transporting them between cellular membranes.
Biological function summary

ORP1 engages in intracellular lipid transport influencing lipid homeostasis and vesicular trafficking. It is part of a protein complex that associates with late endosomes and the endoplasmic reticulum membrane. ORP1 contributes to the regulation of endosomal positioning and transport by modulating the activity of motor proteins on endosomal surfaces. This protein interaction assists in maintaining an appropriate balance of cholesterol and other sterols within cells impacting overall cellular health.

Pathways

Several cellular mechanisms involve ORP1 including the sterol regulatory element-binding protein (SREBP) pathway and the PI3K/Akt signaling pathway. ORP1 interacts with NPC1 a protein critical for cholesterol transport within the SREBP pathway to facilitate the regulation of lipid metabolism. Furthermore ORP1 influences the PI3K/Akt signaling pathway by interacting with other lipid-related proteins coordinating cellular responses to lipid levels and adapting to changes in nutritional states.

Abnormalities related to ORP1 function have connections to neurodegenerative diseases and metabolic syndromes. In neurodegenerative conditions like Niemann-Pick disease ORP1 interacts with the NPC1 and NPC2 proteins both of which are important for normal cholesterol trafficking. In metabolic disorders this protein’s role in lipid regulation links it to obesity and associated metabolic syndrome where dysregulation of lipid storage and transport is common. These connections render ORP1 a potential target for therapeutic intervention in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (By similarity). Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A (PubMed : 16176980). Binds 25-hydroxycholesterol and cholesterol (PubMed : 17428193).
See full target information OSBPL1A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 91: PubMed28077646

2017

Adenovirus Modulates Toll-Like Receptor 4 Signaling by Reprogramming ORP1L-VAP Protein Contacts for Cholesterol Transport from Endosomes to the Endoplasmic Reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas L Cianciola,Stacey Chung,Danny Manor,Cathleen R Carlin
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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