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Rabbit Recombinant Monoclonal ORP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

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Images

Western blot - Anti-ORP1 antibody [EPR8646] (AB131165), expandable thumbnail
  • Western blot - Anti-ORP1 antibody [EPR8646] (AB131165), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ORP1 antibody [EPR8646] (AB131165), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] (AB131165), expandable thumbnail
  • Western blot - Anti-ORP1 antibody [EPR8646] (AB131165), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Tested
Mouse
Not recommended
Tested
Expected
Expected
Rat
Not recommended
Tested
Expected
Expected

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/10000
Notes

-

Species
Rat
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (By similarity). Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A (PubMed:16176980). Binds 25-hydroxycholesterol and cholesterol (PubMed:17428193).

Alternative names

ORP1, OSBP8, OSBPL1, OSBPL1B, OSBPL1A, Oxysterol-binding protein-related protein 1, ORP-1, OSBP-related protein 1

Recommended products

Rabbit Recombinant Monoclonal ORP1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8646
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ORP1 also known as oxysterol-binding protein-related protein 1 is a member of the OSBP (oxysterol-binding protein) family. It functions as a lipid transfer protein involved in the regulation of intracellular lipid dynamics. ORP1 weighs approximately 108 kDa and is ubiquitously expressed in various tissues with higher levels in the liver and brain. The protein interacts with lipids and plays a role in the control of cholesterol and phosphoinositide levels by transporting them between cellular membranes.

Biological function summary

ORP1 engages in intracellular lipid transport influencing lipid homeostasis and vesicular trafficking. It is part of a protein complex that associates with late endosomes and the endoplasmic reticulum membrane. ORP1 contributes to the regulation of endosomal positioning and transport by modulating the activity of motor proteins on endosomal surfaces. This protein interaction assists in maintaining an appropriate balance of cholesterol and other sterols within cells impacting overall cellular health.

Pathways

Several cellular mechanisms involve ORP1 including the sterol regulatory element-binding protein (SREBP) pathway and the PI3K/Akt signaling pathway. ORP1 interacts with NPC1 a protein critical for cholesterol transport within the SREBP pathway to facilitate the regulation of lipid metabolism. Furthermore ORP1 influences the PI3K/Akt signaling pathway by interacting with other lipid-related proteins coordinating cellular responses to lipid levels and adapting to changes in nutritional states.

Associated diseases and disorders

Abnormalities related to ORP1 function have connections to neurodegenerative diseases and metabolic syndromes. In neurodegenerative conditions like Niemann-Pick disease ORP1 interacts with the NPC1 and NPC2 proteins both of which are important for normal cholesterol trafficking. In metabolic disorders this protein’s role in lipid regulation links it to obesity and associated metabolic syndrome where dysregulation of lipid storage and transport is common. These connections render ORP1 a potential target for therapeutic intervention in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-ORP1 antibody [EPR8646] (ab131165), expandable thumbnail

    Western blot - Anti-ORP1 antibody [EPR8646] (ab131165)

    Two mRNA variants produce a 108 kDa long ORP1 (ORP1L) and a 50 KDa short ORP1 (ORP1S) separately, which is consistent with what has been described in PMID: 12631712.

    All lanes: Western blot - Anti-ORP1 antibody [EPR8646] (ab131165) at 1/10000 dilution

    Lane 1: A549 (Human lung carcinoma epithelial cell) whole cell lysates at 20 µg

    Lane 2: U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates at 20 µg

    Lane 3: Mouse brain lysates at 20 µg

    Lane 4: Mouse heart lysates at 20 µg

    Lane 5: Rat brain lysates at 20 µg

    Lane 6: Rat heart lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 108 kDa

    Observed band size: 108 kDa, 50 kDa

  • Western blot - Anti-ORP1 antibody [EPR8646] (ab131165), expandable thumbnail

    Western blot - Anti-ORP1 antibody [EPR8646] (ab131165)

    Two mRNA variants produce a 108 kDa long ORP1 (ORP1L) and a 50 KDa short ORP1 (ORP1S) separately, which is consistent with what has been described in PMID: 12631712.

    All lanes: Western blot - Anti-ORP1 antibody [EPR8646] (ab131165) at 1/1000 dilution

    All lanes: 293T (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 108 kDa

    Observed band size: 108 kDa, 50 kDa

  • Flow Cytometry (Intracellular) - Anti-ORP1 antibody [EPR8646] (ab131165), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ORP1 antibody [EPR8646] (ab131165)

    Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1/60 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] (ab131165), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] (ab131165)

    Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1:200 dilution (2.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-ORP1 antibody [EPR8646] (ab131165), expandable thumbnail

    Western blot - Anti-ORP1 antibody [EPR8646] (ab131165)

    All lanes: Western blot - Anti-ORP1 antibody [EPR8646] (ab131165) at 1/1000 dilution

    Lane 1: A549 cell lysate at 10 µg

    Lane 2: 293T cell lysate at 10 µg

    Lane 3: U87-MG cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 108 kDa

    Observed band size: 50-108 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] (ab131165), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] (ab131165)

    ab131165 (unpurified) staining ORP1 in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a dilution of 1/1000.

    DAPI was used as a nuclear counterstain and the negative control was PBS only.

  • Flow Cytometry (Intracellular) - Anti-ORP1 antibody [EPR8646] (ab131165), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ORP1 antibody [EPR8646] (ab131165)

    ab131165 (unpurified) at 1/10 dilution staining ORP1 in permeabilized A549 cells by intracellular flow cytometry (red). Rabbit IgG negative control (green).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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