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AB248366

Anti-ORP1 antibody [EPR8646] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal ORP1 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

ORP1, OSBP8, OSBPL1, OSBPL1B, OSBPL1A, Oxysterol-binding protein-related protein 1, ORP-1, OSBP-related protein 1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] - BSA and Azide free (AB248366)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ORP1 antibody [EPR8646] - BSA and Azide free (AB248366)

Immunocytochemistry/ Immunofluorescence analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1 : 200 dilution (2.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (anti orp1 antibody epr8646 immunocytochemistry a549 human)

Flow Cytometry (Intracellular) - Anti-ORP1 antibody [EPR8646] - BSA and Azide free (AB248366)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ORP1 antibody [EPR8646] - BSA and Azide free (AB248366)

Intracellular Flow Cytometry analysis of A549 (Human lung carcinoma epithelial cell) cells labeling ORP1 with purified ab131165 at 1/60 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab131165)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8646

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab248366 is the carrier-free version of ab131165.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ORP1 also known as oxysterol-binding protein-related protein 1 is a member of the OSBP (oxysterol-binding protein) family. It functions as a lipid transfer protein involved in the regulation of intracellular lipid dynamics. ORP1 weighs approximately 108 kDa and is ubiquitously expressed in various tissues with higher levels in the liver and brain. The protein interacts with lipids and plays a role in the control of cholesterol and phosphoinositide levels by transporting them between cellular membranes.
Biological function summary

ORP1 engages in intracellular lipid transport influencing lipid homeostasis and vesicular trafficking. It is part of a protein complex that associates with late endosomes and the endoplasmic reticulum membrane. ORP1 contributes to the regulation of endosomal positioning and transport by modulating the activity of motor proteins on endosomal surfaces. This protein interaction assists in maintaining an appropriate balance of cholesterol and other sterols within cells impacting overall cellular health.

Pathways

Several cellular mechanisms involve ORP1 including the sterol regulatory element-binding protein (SREBP) pathway and the PI3K/Akt signaling pathway. ORP1 interacts with NPC1 a protein critical for cholesterol transport within the SREBP pathway to facilitate the regulation of lipid metabolism. Furthermore ORP1 influences the PI3K/Akt signaling pathway by interacting with other lipid-related proteins coordinating cellular responses to lipid levels and adapting to changes in nutritional states.

Abnormalities related to ORP1 function have connections to neurodegenerative diseases and metabolic syndromes. In neurodegenerative conditions like Niemann-Pick disease ORP1 interacts with the NPC1 and NPC2 proteins both of which are important for normal cholesterol trafficking. In metabolic disorders this protein’s role in lipid regulation links it to obesity and associated metabolic syndrome where dysregulation of lipid storage and transport is common. These connections render ORP1 a potential target for therapeutic intervention in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds phospholipids; exhibits strong binding to phosphatidic acid and weak binding to phosphatidylinositol 3-phosphate (By similarity). Stabilizes GTP-bound RAB7A on late endosomes/lysosomes and alters functional properties of late endocytic compartments via its interaction with RAB7A (PubMed : 16176980). Binds 25-hydroxycholesterol and cholesterol (PubMed : 17428193).
See full target information OSBPL1A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 24:457 PubMed36380881

2022

Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Shou-Ji Cao,Wan-Li Ge,Ling-Dong Meng,Qun Chen,Yi Miao,Kui-Rong Jiang,Jing-Jing Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com