Rabbit Polyclonal ORP150 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HYOU1 aa 650 to C-terminus.
IgG
Rabbit
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS
Liquid
Polyclonal
WB | IHC-P | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Chinese hamster | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 0.2-2 µg/mL | Notes - |
Species Human | Dilution info 0.2-2 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chinese hamster | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 5-20 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Chinese hamster | Dilution info - | Notes - |
Select an associated product type
Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
GRP170, HSPH4, ORP150, HYOU1, Hypoxia up-regulated protein 1, 150 kDa oxygen-regulated protein, 170 kDa glucose-regulated protein, Heat shock protein family H member 4, ORP-150, GRP-170
Rabbit Polyclonal ORP150 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HYOU1 aa 650 to C-terminus.
IgG
Rabbit
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS
Liquid
Polyclonal
Affinity purification Immunogen
ab232902 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
ORP150 also known as Heat Shock Protein 12A (HSPA12A) is a molecular chaperone with an approximate mass of 150 kDa. This protein plays a critical role in protein folding and preventing misfolded proteins in the endoplasmic reticulum (ER). ORP150 is mainly expressed in cells that face challenging conditions like those in the brain heart and tumors. Its expression indeed increases under stress conditions that cause protein unfolding such as hypoxia or glucose deprivation.
ORP150 functions to aid cellular adaptation during stress by maintaining protein homeostasis within the ER. It is recognized as part of the heat shock protein family which is known for its involvement in cellular protection mechanisms. By stabilizing polypeptides and proteins ORP150 contributes significantly to cellular survival under various stress conditions. Moreover its expression under hypoxic conditions suggests ORP150's integral role in such stressful environments helping to ameliorate oxidative damage to cells.
ORP150 integrates into the unfolded protein response (UPR) and ER-associated degradation (ERAD) pathways. These pathways help in the maintenance of protein quality control within the ER. Within the UPR ORP150 collaborates with proteins like GRP78 another chaperone to manage misfolded proteins and ensure proper maturation of nascent chains. Furthermore ORP150 participates in ERAD where it assists in targeting misfolded proteins for degradation therefore managing cellular stress and maintaining cellular health.
ORP150 has associations with neurodegenerative diseases like Alzheimer's disease and cardiovascular disorders. In conditions such as Alzheimer's the accumulation of misfolded proteins can exacerbate the disease state where ORP150 potentially interacts with amyloid precursor protein (APP) to regulate its folding and processing. Similarly in cardiovascular diseases ORP150 may influence the function of related proteins like GRP78 as both play roles in protecting cardiac cells from ischemic damage. The consistent association of ORP150 expression with stressed cellular environments suggests its importance in the pathology and management of these conditions.
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This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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All lanes: Western blot - Anti-ORP150 antibody (ab232902) at 2 µg/mL
All lanes: Recombinant human ORP150 protein
Predicted band size: 111 kDa
All lanes: Western blot - Anti-ORP150 antibody (ab232902) at 2 µg/mL
All lanes: Human serum
Predicted band size: 111 kDa
All lanes: Western blot - Anti-ORP150 antibody (ab232902) at 2 µg/mL
All lanes: Human lung lysate
Predicted band size: 111 kDa
All lanes: Western blot - Anti-ORP150 antibody (ab232902) at 2 µg/mL
All lanes: Mouse heart lysate
Predicted band size: 111 kDa
Formalin-fixed, paraffin-embedded human pancreas tissue stained for ORP150 using ab232902 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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