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Rabbit Polyclonal ORP150 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HYOU1 aa 650 to C-terminus.

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Images

Western blot - Anti-ORP150 antibody (AB232902), expandable thumbnail
  • Western blot - Anti-ORP150 antibody (AB232902), expandable thumbnail
  • Western blot - Anti-ORP150 antibody (AB232902), expandable thumbnail
  • Western blot - Anti-ORP150 antibody (AB232902), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody (AB232902), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human HYOU1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4L1

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Mouse
Tested
Expected
Chinese hamster
Predicted
Predicted

Tested
Tested

Species

Mouse

Dilution info

0.2-2 µg/mL

Notes

-

Species

Human

Dilution info

0.2-2 µg/mL

Notes

-

Predicted
Predicted

Species

Chinese hamster

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

5-20 µg/mL

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Chinese hamster

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.

Alternative names

Recommended products

Rabbit Polyclonal ORP150 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HYOU1 aa 650 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human HYOU1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y4L1
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab232902 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ORP150 also known as Heat Shock Protein 12A (HSPA12A) is a molecular chaperone with an approximate mass of 150 kDa. This protein plays a critical role in protein folding and preventing misfolded proteins in the endoplasmic reticulum (ER). ORP150 is mainly expressed in cells that face challenging conditions like those in the brain heart and tumors. Its expression indeed increases under stress conditions that cause protein unfolding such as hypoxia or glucose deprivation.

Biological function summary

ORP150 functions to aid cellular adaptation during stress by maintaining protein homeostasis within the ER. It is recognized as part of the heat shock protein family which is known for its involvement in cellular protection mechanisms. By stabilizing polypeptides and proteins ORP150 contributes significantly to cellular survival under various stress conditions. Moreover its expression under hypoxic conditions suggests ORP150's integral role in such stressful environments helping to ameliorate oxidative damage to cells.

Pathways

ORP150 integrates into the unfolded protein response (UPR) and ER-associated degradation (ERAD) pathways. These pathways help in the maintenance of protein quality control within the ER. Within the UPR ORP150 collaborates with proteins like GRP78 another chaperone to manage misfolded proteins and ensure proper maturation of nascent chains. Furthermore ORP150 participates in ERAD where it assists in targeting misfolded proteins for degradation therefore managing cellular stress and maintaining cellular health.

Associated diseases and disorders

ORP150 has associations with neurodegenerative diseases like Alzheimer's disease and cardiovascular disorders. In conditions such as Alzheimer's the accumulation of misfolded proteins can exacerbate the disease state where ORP150 potentially interacts with amyloid precursor protein (APP) to regulate its folding and processing. Similarly in cardiovascular diseases ORP150 may influence the function of related proteins like GRP78 as both play roles in protecting cardiac cells from ischemic damage. The consistent association of ORP150 expression with stressed cellular environments suggests its importance in the pathology and management of these conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Western blot - Anti-ORP150 antibody (ab232902), expandable thumbnail

    Western blot - Anti-ORP150 antibody (ab232902)

    All lanes: Western blot - Anti-ORP150 antibody (ab232902) at 2 µg/mL

    All lanes: Recombinant human ORP150 protein

    Predicted band size: 111 kDa

  • Western blot - Anti-ORP150 antibody (ab232902), expandable thumbnail

    Western blot - Anti-ORP150 antibody (ab232902)

    All lanes: Western blot - Anti-ORP150 antibody (ab232902) at 2 µg/mL

    All lanes: Human serum

    Predicted band size: 111 kDa

  • Western blot - Anti-ORP150 antibody (ab232902), expandable thumbnail

    Western blot - Anti-ORP150 antibody (ab232902)

    All lanes: Western blot - Anti-ORP150 antibody (ab232902) at 2 µg/mL

    All lanes: Human lung lysate

    Predicted band size: 111 kDa

  • Western blot - Anti-ORP150 antibody (ab232902), expandable thumbnail

    Western blot - Anti-ORP150 antibody (ab232902)

    All lanes: Western blot - Anti-ORP150 antibody (ab232902) at 2 µg/mL

    All lanes: Mouse heart lysate

    Predicted band size: 111 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody (ab232902), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody (ab232902)

    Formalin-fixed, paraffin-embedded human pancreas tissue stained for ORP150 using ab232902 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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