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AB232902

Anti-ORP150 antibody

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(1 Publication)

Rabbit Polyclonal ORP150 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HYOU1 aa 650 to C-terminus.

View Alternative Names

GRP170, HSPH4, ORP150, HYOU1, Hypoxia up-regulated protein 1, 150 kDa oxygen-regulated protein, 170 kDa glucose-regulated protein, Heat shock protein family H member 4, ORP-150, GRP-170

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody (AB232902)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody (AB232902)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for ORP150 using ab232902 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Western blot - Anti-ORP150 antibody (AB232902)
  • WB

Supplier Data

Western blot - Anti-ORP150 antibody (AB232902)

All lanes:

Western blot - Anti-ORP150 antibody (ab232902) at 2 µg/mL

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Human serum

Predicted band size: 111 kDa

false

Western blot - Anti-ORP150 antibody (AB232902)
  • WB

Supplier Data

Western blot - Anti-ORP150 antibody (AB232902)

All lanes:

Western blot - Anti-ORP150 antibody (ab232902) at 2 µg/mL

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Human lung lysate

Predicted band size: 111 kDa

false

Western blot - Anti-ORP150 antibody (AB232902)
  • WB

Supplier Data

Western blot - Anti-ORP150 antibody (AB232902)

All lanes:

Western blot - Anti-ORP150 antibody (ab232902) at 2 µg/mL

All lanes:

Recombinant human ORP150 protein

Predicted band size: 111 kDa

false

Western blot - Anti-ORP150 antibody (AB232902)
  • WB

Supplier Data

Western blot - Anti-ORP150 antibody (AB232902)

All lanes:

Western blot - Anti-ORP150 antibody (ab232902) at 2 µg/mL

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Mouse heart lysate

Predicted band size: 111 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HYOU1 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4L1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab232902 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.
Storage buffer
pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ORP150 also known as Heat Shock Protein 12A (HSPA12A) is a molecular chaperone with an approximate mass of 150 kDa. This protein plays a critical role in protein folding and preventing misfolded proteins in the endoplasmic reticulum (ER). ORP150 is mainly expressed in cells that face challenging conditions like those in the brain heart and tumors. Its expression indeed increases under stress conditions that cause protein unfolding such as hypoxia or glucose deprivation.
Biological function summary

ORP150 functions to aid cellular adaptation during stress by maintaining protein homeostasis within the ER. It is recognized as part of the heat shock protein family which is known for its involvement in cellular protection mechanisms. By stabilizing polypeptides and proteins ORP150 contributes significantly to cellular survival under various stress conditions. Moreover its expression under hypoxic conditions suggests ORP150's integral role in such stressful environments helping to ameliorate oxidative damage to cells.

Pathways

ORP150 integrates into the unfolded protein response (UPR) and ER-associated degradation (ERAD) pathways. These pathways help in the maintenance of protein quality control within the ER. Within the UPR ORP150 collaborates with proteins like GRP78 another chaperone to manage misfolded proteins and ensure proper maturation of nascent chains. Furthermore ORP150 participates in ERAD where it assists in targeting misfolded proteins for degradation therefore managing cellular stress and maintaining cellular health.

ORP150 has associations with neurodegenerative diseases like Alzheimer's disease and cardiovascular disorders. In conditions such as Alzheimer's the accumulation of misfolded proteins can exacerbate the disease state where ORP150 potentially interacts with amyloid precursor protein (APP) to regulate its folding and processing. Similarly in cardiovascular diseases ORP150 may influence the function of related proteins like GRP78 as both play roles in protecting cardiac cells from ischemic damage. The consistent association of ORP150 expression with stressed cellular environments suggests its importance in the pathology and management of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
See full target information HYOU1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Viruses 15: PubMed36851534

2023

Obesity Induces an Impaired Placental Antiviral Immune Response in Pregnant Women Infected with Zika Virus.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Cláudia Calvielli Castelo Branco,Emily Araujo De Oliveira,Nátalli Zanete Pereira,Ricardo Wesley Alberca,Amaro Nunes Duarte-Neto,Luiz Fernando Ferraz Da Silva,Fernanda Guedes Luiz,Naiura Vieira Pereira,Mirian Nacagami Sotto,Naiara Naiana Dejani,Patrícia Helen Carvalho Rondó,Elyzabeth Avvad-Portari,Zilton Farias Meira De Vasconcelos,Alberto José da Silva Duarte,Tamiris Azamor,Maria Notomi Sato
View all publications
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