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AB134944

Anti-ORP150 antibody [EP5891]

5

(1 Review)

|

(6 Publications)

Rabbit Monoclonal ORP150 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.

View Alternative Names

GRP170, HSPH4, ORP150, HYOU1, Hypoxia up-regulated protein 1, 150 kDa oxygen-regulated protein, 170 kDa glucose-regulated protein, Heat shock protein family H member 4, ORP-150, GRP-170

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-ORP150 antibody [EP5891] (AB134944)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ORP150 antibody [EP5891] (AB134944)

Immunofluorescence staining of MCF7 cells, labelling ORP150 using ab134944 at a 1/250 dilution (red). Cell nuclei are shown in blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EP5891] (AB134944)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EP5891] (AB134944)

Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human liver tissue, labelling ORP150 using ab134944 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-ORP150 antibody [EP5891] (AB134944)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ORP150 antibody [EP5891] (AB134944)

Intracellular Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling ORP150 with unpurified ab134944 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EP5891] (AB134944)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ORP150 antibody [EP5891] (AB134944)

Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human colon tissue, labelling ORP150 using ab134944 at a 1/250 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-ORP150 antibody [EP5891] (AB134944)
  • IP

Lab

Immunoprecipitation - Anti-ORP150 antibody [EP5891] (AB134944)

purifiedab134944 at 1/50 dilution (2μg) immunoprecipitating ORP150 in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab134944 + HeLa whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab134944 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/2000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 150 kDa

All lanes:

Immunoprecipitation - Anti-ORP150 antibody [EP5891] (ab134944)

Predicted band size: 111 kDa

false

Western blot - Anti-ORP150 antibody [EP5891] (AB134944)
  • WB

Unknown

Western blot - Anti-ORP150 antibody [EP5891] (AB134944)

All lanes:

Western blot - Anti-ORP150 antibody [EP5891] (ab134944) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

SH-SY5Y cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Lane 5:

293T cell lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 111 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP5891

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ORP150 also known as Heat Shock Protein 12A (HSPA12A) is a molecular chaperone with an approximate mass of 150 kDa. This protein plays a critical role in protein folding and preventing misfolded proteins in the endoplasmic reticulum (ER). ORP150 is mainly expressed in cells that face challenging conditions like those in the brain heart and tumors. Its expression indeed increases under stress conditions that cause protein unfolding such as hypoxia or glucose deprivation.
Biological function summary

ORP150 functions to aid cellular adaptation during stress by maintaining protein homeostasis within the ER. It is recognized as part of the heat shock protein family which is known for its involvement in cellular protection mechanisms. By stabilizing polypeptides and proteins ORP150 contributes significantly to cellular survival under various stress conditions. Moreover its expression under hypoxic conditions suggests ORP150's integral role in such stressful environments helping to ameliorate oxidative damage to cells.

Pathways

ORP150 integrates into the unfolded protein response (UPR) and ER-associated degradation (ERAD) pathways. These pathways help in the maintenance of protein quality control within the ER. Within the UPR ORP150 collaborates with proteins like GRP78 another chaperone to manage misfolded proteins and ensure proper maturation of nascent chains. Furthermore ORP150 participates in ERAD where it assists in targeting misfolded proteins for degradation therefore managing cellular stress and maintaining cellular health.

ORP150 has associations with neurodegenerative diseases like Alzheimer's disease and cardiovascular disorders. In conditions such as Alzheimer's the accumulation of misfolded proteins can exacerbate the disease state where ORP150 potentially interacts with amyloid precursor protein (APP) to regulate its folding and processing. Similarly in cardiovascular diseases ORP150 may influence the function of related proteins like GRP78 as both play roles in protecting cardiac cells from ischemic damage. The consistent association of ORP150 expression with stressed cellular environments suggests its importance in the pathology and management of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding.
See full target information HYOU1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

The Journal of clinical investigation 134: PubMed39264847

2024

Lactate supports Treg function and immune balance via MGAT1 effects on N-glycosylation in the mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Jinren Zhou,Jian Gu,Qufei Qian,Yigang Zhang,Tianning Huang,Xiangyu Li,Zhuoqun Liu,Qing Shao,Yuan Liang,Lei Qiao,Xiaozhang Xu,Qiuyang Chen,Zibo Xu,Yu Li,Ji Gao,Yufeng Pan,Yiming Wang,Roderick O'Connor,Keli L Hippen,Ling Lu,Bruce R Blazar

Bioengineered 12:8753-8767 PubMed34704918

2021

Long non-coding RNA KCNQ1OT1 facilitates the progression of cervical cancer and tumor growth through modulating miR-296-5p/HYOU1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liu,Yingmei Wang

Oncology letters 16:1943-1952 PubMed30034552

2018

Effect of Derris scandens extract on a human hepatocellular carcinoma cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Duanghathai Kuljittichanok,Penchatr Diskul-Na-Ayudthaya,Churat Weeraphan,Daranee Chokchaichamnankit,Khajeelak Chiablaem,Kriengsak Lirdprapamongkol,Jisnuson Svasti,Chantragan Srisomsap

Acta ophthalmologica 96:e460-e467 PubMed29098793

2017

Association of 150-kDa oxygen-regulated protein with vascular endothelial growth factor in proliferative diabetic retinopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed M Abu El-Asrar,Ajmal Ahmad,Kaiser Alam,Emilie Bittoun,Mohammad Mairaj Siddiquei,Ghulam Mohammad,Ahmed Mousa,Gert De Hertogh,Ghislain Opdenakker

Journal of virology 88:9429-41 PubMed24920810

2014

Modulation of Kaposi's sarcoma-associated herpesvirus interleukin-6 function by hypoxia-upregulated protein 1.

Applications

Unspecified application

Species

Unspecified reactive species

Louise Giffin,Feng Yan,M Ben Major,Blossom Damania

Acta neurologica Belgica 114:187-94 PubMed24085542

2013

Proteomic analysis of meningiomas.

Applications

WB

Species

Human

G Q Cui,A H Jiao,C M Xiu,Y B Wang,P Sun,L M Zhang,X G Li
View all publications
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