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AB228990

Anti-ORP8 antibody

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(1 Publication)

Rabbit Polyclonal ORP8 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human OSBPL8.

View Alternative Names

KIAA1451, ORP8, OSBP10, OSBPL8, Oxysterol-binding protein-related protein 8, ORP-8, OSBP-related protein 8

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ORP8 antibody (AB228990)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ORP8 antibody (AB228990)

Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ORP8 (green) using ab228990 (1/500 dilution) in ICC/IF.

Counterstain : Hoechst 33342 (blue).

Western blot - Anti-ORP8 antibody (AB228990)
  • WB

Supplier Data

Western blot - Anti-ORP8 antibody (AB228990)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-ORP8 antibody (ab228990) at 1/10000 dilution

All lanes:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

Predicted band size: 101 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human OSBPL8. The exact immunogen used to generate this antibody is proprietary information.

Q9BZF1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oxysterol-binding protein-related protein 8 (ORP8) also known as OSBP-related protein 8 is a member of the oxysterol-binding protein (OSBP) family. This protein has a mass of around 104 kDa. ORP8 is expressed in various tissues with notable expression in the liver and brain. Mechanically ORP8 functions as a lipid transporter mediating cholesterol and phosphoinositide transfer between cellular membranes. It contains a pleckstrin homology domain and an oxysterol-binding domain which facilitate its role in lipid exchange and signaling processes.
Biological function summary

ORP8 influences membrane lipid composition and cellular lipid metabolism. It regulates cholesterol homeostasis and participates in lipid signaling pathways. Although ORP8 is not a known component of larger protein complexes it interacts with other lipid-regulating entities modulating cellular responses to lipid fluctuations. This regulation maintains cellular energy balance and membrane integrity critical aspects of cellular physiology.

Pathways

ORP8 actively contributes to cholesterol metabolism and phosphoinositide signaling pathways. It interacts directly with key regulators such as the endoplasmic reticulum-resident protein VAP and phosphatidylinositol 4-kinase. These interactions help orchestrate cholesterol and lipid distribution within cells linking ORP8 to broader metabolic processes and lipid signaling events. These pathways highlight ORP8's role in maintaining cellular lipid equilibrium.

ORP8 potentially associates with conditions like atherosclerosis and liver disease. Dysregulation of its lipid transport functions may lead to abnormal lipid accumulation which underpins atherosclerosis development. Additionally ORP8 interacts with proteins implicated in lipid metabolism disorders connecting it to liver disease pathogenesis. Its interactions with VAP and other metabolic proteins emphasize the importance of ORP8 in disease contexts where lipid balance is disrupted.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane : specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed : 26206935). Binds oxysterol, 25-hydroxycholesterol and cholesterol (PubMed : 17428193, PubMed : 17991739, PubMed : 21698267).
See full target information OSBPL8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Immune network 23:e34 PubMed37670811

2023

Tumor Promoting Function of DUSP10 in Non-Small Cell Lung Cancer Is Associated With Tumor-Promoting Cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Wei,Chin Wen Png,Madhushanee Weerasooriya,Heng Li,Chenchen Zhu,Guiping Chen,Chuan Xu,Yongliang Zhang,Xiaohong Xu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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