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AB225915

Anti-OS9 antibody

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(1 Publication)

Rabbit Polyclonal OS9 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Protein OS-9 aa 200-450.

View Alternative Names

Protein OS-9, Amplified in osteosarcoma 9, OS9

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody (AB225915)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody (AB225915)

Paraffin-embedded human testis tissue stained for OS9 using ab225915 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody (AB225915)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody (AB225915)

Paraffin-embedded human lung cancer tissue stained for OS9 using ab225915 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-OS9 antibody (AB225915)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OS9 antibody (AB225915)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for OS9 (green) using ab225915 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-OS9 antibody (AB225915)
  • WB

Supplier Data

Western blot - Anti-OS9 antibody (AB225915)

All lanes:

Western blot - Anti-OS9 antibody (ab225915) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 2:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 76 kDa

Observed band size: 76 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human Protein OS-9 aa 200-450. The exact immunogen used to generate this antibody is proprietary information.

Q13438

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OS9 also known as osteosarcoma amplified 9 protein has a molecular mass of approximately 84 kDa. The protein primarily locates in the endoplasmic reticulum (ER). OS9 functions as an ER-associated protein that plays an important role in the recognition and targeting of misfolded glycoproteins for degradation through the ER-associated degradation (ERAD) pathway. It assembles as part of the ERAD machinery through interactions with glycoproteins via its mannose 6-phosphate receptor homology (MRH) domain directly influencing the fate of proteins that fail proper folding.
Biological function summary

OS9 plays an important role in protein quality control within the cell. It forms part of a complex that detects and binds misfolded glycoproteins therefore marking them for transport to the proteasome for degradation. This function aids in maintaining cellular homeostasis by preventing the accumulation of defective proteins that can lead to cellular stress and dysfunction. The protein works in coordination with other ER chaperones and components such as SEL1L and HERPUD1 facilitating efficient substrate delivery to the degradation pathway.

Pathways

OS9 integrates into the ERAD pathway which is important for managing the protein folding load within the ER. This process is essential in managing and mitigating ER stress linked to the unfolded protein response (UPR). In particular OS9 interacts with ER mannosyl-oligosaccharide 12-alpha-mannosidase (MAN1B1) to trim mannose a vital step in determining protein fate within this pathway. Additionally OS9 links with proteins such as XBP1 helping regulate cellular stress responses.

OS9 has implications in neurodegenerative diseases and cancer. Misregulation or mutations affecting its function can contribute to diseases characterized by protein misfolding such as Alzheimer's disease where it might interact with other proteins involved in amyloid precursor protein (APP) processing. Additionally in the context of cancer overexpression correlates with tumor progression in certain cancers as it may influence pathways involving HERPUD1 affecting cell survival and proliferation under stress conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
See full target information Protein OS-9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 46: PubMed33982764

2021

Long non‑coding RNA NEAT1 regulates glioma cell proliferation and apoptosis by competitively binding to microRNA‑324‑5p and upregulating KCTD20 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jiale Zhang,Yangyang Li,Yuqi Liu,Guangzhi Xu,Yue Hei,Xiaoming Lu,Weiping Liu
View all publications
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