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AB109510

Anti-OS9 antibody [EPR4272(2)]

5

(1 Review)

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(21 Publications)

Rabbit Recombinant Monoclonal OS9 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 21 publications.

View Alternative Names

Protein OS-9, Amplified in osteosarcoma 9, OS9

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody [EPR4272(2)] (AB109510)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OS9 antibody [EPR4272(2)] (AB109510)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using ab109510 at a dilution of 1/100.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-OS9 antibody [EPR4272(2)] (AB109510)
  • WB

Unknown

Western blot - Anti-OS9 antibody [EPR4272(2)] (AB109510)

All lanes:

Western blot - Anti-OS9 antibody [EPR4272(2)] (ab109510) at 1/10000 dilution

Lane 1:

NIH3T3 cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

HL-60 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 76 kDa

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  • Carrier free

    Anti-OS9 antibody [EPR4272(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4272(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OS9 also known as osteosarcoma amplified 9 protein has a molecular mass of approximately 84 kDa. The protein primarily locates in the endoplasmic reticulum (ER). OS9 functions as an ER-associated protein that plays an important role in the recognition and targeting of misfolded glycoproteins for degradation through the ER-associated degradation (ERAD) pathway. It assembles as part of the ERAD machinery through interactions with glycoproteins via its mannose 6-phosphate receptor homology (MRH) domain directly influencing the fate of proteins that fail proper folding.
Biological function summary

OS9 plays an important role in protein quality control within the cell. It forms part of a complex that detects and binds misfolded glycoproteins therefore marking them for transport to the proteasome for degradation. This function aids in maintaining cellular homeostasis by preventing the accumulation of defective proteins that can lead to cellular stress and dysfunction. The protein works in coordination with other ER chaperones and components such as SEL1L and HERPUD1 facilitating efficient substrate delivery to the degradation pathway.

Pathways

OS9 integrates into the ERAD pathway which is important for managing the protein folding load within the ER. This process is essential in managing and mitigating ER stress linked to the unfolded protein response (UPR). In particular OS9 interacts with ER mannosyl-oligosaccharide 12-alpha-mannosidase (MAN1B1) to trim mannose a vital step in determining protein fate within this pathway. Additionally OS9 links with proteins such as XBP1 helping regulate cellular stress responses.

OS9 has implications in neurodegenerative diseases and cancer. Misregulation or mutations affecting its function can contribute to diseases characterized by protein misfolding such as Alzheimer's disease where it might interact with other proteins involved in amyloid precursor protein (APP) processing. Additionally in the context of cancer overexpression correlates with tumor progression in certain cancers as it may influence pathways involving HERPUD1 affecting cell survival and proliferation under stress conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lectin which functions in endoplasmic reticulum (ER) quality control and ER-associated degradation (ERAD). May bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. Possible targets include TRPV4.
See full target information Protein OS-9
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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 301:108369 PubMed40024475

2025

Requirements for nuclear GRP78 transcriptional regulatory activities and interaction with nuclear GRP94.

Applications

Unspecified application

Species

Unspecified reactive species

Ze Liu,Dat P Ha,Liangguang Leo Lin,Ling Qi,Amy S Lee

Nature communications 15:9244 PubMed39455574

2024

Regulation of hepatic inclusions and fibrinogen biogenesis by SEL1L-HRD1 ERAD.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenfeng Song,Pattaraporn Thepsuwan,Woosuk Steve Hur,Mauricio Torres,Shuangcheng Alivia Wu,Xiaoqiong Wei,Nusrat Jahan Tushi,Juncheng Wei,Francesca Ferraresso,Adrienne W Paton,James C Paton,Ze Zheng,Kezhong Zhang,Deyu Fang,Christian J Kastrup,Sunil Jaiman,Matthew James Flick,Shengyi Sun

JCI insight 9: PubMed39352758

2024

Purkinje cell-specific deficiency in SEL1L-hrd1 endoplasmic reticulum-associated degradation causes progressive cerebellar ataxia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mauricio Torres,Brent Pederson,Hui Wang,Liangguang Leo Lin,Huilun Helen Wang,Amara Bugarin-Lapuz,Zhen Zhao,Ling Qi

Molecular biology of the cell 35:ar59 PubMed38446639

2024

Loss of Grp170 results in catastrophic disruption of endoplasmic reticulum function.

Applications

Unspecified application

Species

Unspecified reactive species

Melissa J Mann,Chris Melendez-Suchi,Hannah E Vorndran,Maria Sukhoplyasova,Ashley R Flory,Mary Carson Irvine,Anuradha R Iyer,Christopher J Guerriero,Jeffrey L Brodsky,Linda M Hendershot,Teresa M Buck

Nature communications 15:1440 PubMed38365914

2024

SEL1L-HRD1 interaction is required to form a functional HRD1 ERAD complex.

Applications

Unspecified application

Species

Unspecified reactive species

Liangguang Leo Lin,Huilun Helen Wang,Brent Pederson,Xiaoqiong Wei,Mauricio Torres,You Lu,Zexin Jason Li,Xiaodan Liu,Hancheng Mao,Hui Wang,Linyao Elina Zhou,Zhen Zhao,Shengyi Sun,Ling Qi

The Journal of clinical investigation 134: PubMed37943617

2024

Biallelic Cys141Tyr variant of SEL1L is associated with neurodevelopmental disorders, agammaglobulinemia, and premature death.

Applications

Unspecified application

Species

Unspecified reactive species

Denisa Weis,Liangguang L Lin,Huilun H Wang,Zexin Jason Li,Katarina Kusikova,Peter Ciznar,Hermann M Wolf,Alexander Leiss-Piller,Zhihong Wang,Xiaoqiong Wei,Serge Weis,Katarina Skalicka,Gabriela Hrckova,Lubos Danisovic,Andrea Soltysova,Tingxuan T Yang,René Günther Feichtinger,Johannes A Mayr,Ling Qi

The Journal of clinical investigation 134: PubMed37943610

2024

Hypomorphic variants of SEL1L-HRD1 ER-associated degradation are associated with neurodevelopmental disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Huilun H Wang,Liangguang L Lin,Zexin J Li,Xiaoqiong Wei,Omar Askander,Gerarda Cappuccio,Mais O Hashem,Laurence Hubert,Arnold Munnich,Mashael Alqahtani,Qi Pang,Margit Burmeister,You Lu,Karine Poirier,Claude Besmond,Shengyi Sun,Nicola Brunetti-Pierri,Fowzan S Alkuraya,Ling Qi

JCI insight 8: PubMed37345654

2023

Perturbation of endoplasmic reticulum proteostasis triggers tissue injury in the thyroid gland.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohan Zhang,Crystal Young,Xiao-Hui Liao,Samuel Refetoff,Mauricio Torres,Yaron Tomer,Mihaela Stefan-Lifshitz,Hao Zhang,Dennis Larkin,Deyu Fang,Ling Qi,Peter Arvan

Nature communications 14:3132 PubMed37253728

2023

The mechanisms to dispose of misfolded proteins in the endoplasmic reticulum of adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangcheng Alivia Wu,Chenchen Shen,Xiaoqiong Wei,Xiawei Zhang,Siwen Wang,Xinxin Chen,Mauricio Torres,You Lu,Liangguang Leo Lin,Huilun Helen Wang,Allen H Hunter,Deyu Fang,Shengyi Sun,Magdalena I Ivanova,Yi Lin,Ling Qi

Nature cell biology 25:726-739 PubMed37142791

2023

SEL1L-HRD1 endoplasmic reticulum-associated degradation controls STING-mediated innate immunity by limiting the size of the activable STING pool.

Applications

Unspecified application

Species

Unspecified reactive species

Yewei Ji,Yuan Luo,Yating Wu,Yao Sun,Lianfeng Zhao,Zhen Xue,Mengqi Sun,Xiaoqiong Wei,Zinan He,Shuangcheng Alivia Wu,Liangguang Leo Lin,You Lu,Lei Chang,Fei Chen,Siyu Chen,Wei Qian,Xiaoxi Xu,Shengnuo Chen,Dongli Pan,Zhangsen Zhou,Sheng Xia,Chih-Chi Andrew Hu,Tingbo Liang,Ling Qi
View all publications
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