AB192995

Anti-OSBP1 antibody

Name: Anti-OSBP1 antibody (ab192995) | Abcam
Brand: Abcam Limited
SKU: AB192995
Price: 535 USD
Availability: InStock

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Rabbit Polyclonal OSBP1 antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human OSBP aa 700-800.

View Alternative Names

OSBP1, OSBP, Oxysterol-binding protein 1

1 Images

Supplier Data

Western blot - Anti-OSBP1 antibody (AB192995)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-OSBP1 antibody (ab192995) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK1 whole cell lysate at 50 µg

Lane 5:

NIH 3T3 whole cell lysate at 50 µg

Predicted band size: 89 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human OSBP aa 700-800. The exact immunogen used to generate this antibody is proprietary information.

P22059

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab192995 was affinity purified using an epitope specific to OSBP1 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Oxysterol-binding protein 1 (OSBP1) also known as OSBP is a protein with a molecular mass of approximately 97 kilodaltons. It functions as an intracellular lipid transporter that binds oxysterols which are oxygenated derivatives of cholesterol. OSBP1 is involved in lipid exchange between the endoplasmic reticulum (ER) and the Golgi apparatus. It is expressed in various tissues with high levels observed in the liver brain and adipose tissue. OSBP1 facilitates the transport of sterols and phosphatidylinositol 4-phosphate (PI4P) influencing lipid metabolism and membrane formation.

Biological function summary

OSBP1 plays a significant role in maintaining cellular lipid homeostasis and is a part of the larger family of oxysterol-binding proteins. It participates in intracellular lipid transport and signaling aiding in the regulation of cholesterol metabolism. OSBP1 coordinates lipid exchange processes between the ER and other cellular compartments impacting membrane composition and function. Its activity is important for the generation of certain lipid domains contributing to cellular signaling pathways and membrane trafficking.

Pathways

OSBP1 interacts within complex lipid signaling networks and is critically involved in the regulation of the phosphoinositide and cholesterol pathways. It collaborates with proteins such as VAP-A at membrane contact sites to exchange lipids like cholesterol and PI4P. This interaction is key to maintaining lipid gradients essential for multiple cellular functions including signaling and vesicle trafficking. Additionally OSBP1 modulates the activity of downstream phosphatidylinositol 4-kinases affecting phosphoinositide metabolism.

OSBP1's dysregulation associates with abnormalities in lipid metabolism and related diseases. Such alterations can contribute to metabolic disorders like atherosclerosis due to its involvement in cholesterol transport and distribution. Furthermore OSBP1's function is linked to the pathogenesis of Niemann-Pick disease type C where cholesterol accumulation occurs. In these contexts OSBP1 operates in concert with other proteins such as Niemann-Pick C1 (NPC1) that participate in cholesterol trafficking and metabolism.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum : specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum (PubMed : 24209621). Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol (PubMed : 15746430, PubMed : 17428193). Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly (PubMed : 15746430). Regulates cholesterol efflux by decreasing ABCA1 stability (PubMed : 18450749).

See full target information OSBP

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-OSBP1 antibody

Western blot - Anti-OSBP1 antibody (AB192995)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Proteins & Peptides

Primary Antibodies

Product promise