Rabbit Polyclonal OSBPL5/ORP5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human OSBPL5 aa 200-500.
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Publications
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- Journal of lipid research 57:1845-18532016Oxysterol binding protein-related protein 8 mediates the cytotoxicity of 25-hydroxycholesterol.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiwei Li et. al.PubMed 27530118
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human OSBPL5 aa 200-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0X9
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Alternatively Tris-EDTA buffer ( pH8.0) may be used. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane: specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed:23934110, PubMed:26206935). May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes (PubMed:21220512). Binds 25-hydroxycholesterol and cholesterol (PubMed:17428193).
Alternative names
KIAA1534, OBPH1, ORP5, OSBPL5, Oxysterol-binding protein-related protein 5, ORP-5, OSBP-related protein 5, Oxysterol-binding protein homolog 1
Recommended products
Rabbit Polyclonal OSBPL5/ORP5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human OSBPL5 aa 200-500.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human OSBPL5 aa 200-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0X9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
OSBPL5 also known as ORP5 is a member of the oxysterol-binding protein (OSBP) family. This protein weighs approximately 99 kDa and is expressed in various tissues including the liver and the brain. OSBPL5 functions primarily as a lipid transfer protein playing a mechanical role in maintaining lipid homeostasis at membrane contact sites. It facilitates the transfer of phosphatidylserine and phosphatidylinositol-4-phosphate between membranes which is essential for cellular lipid regulation.
- Biological function summary
The OSBP-like protein OSBPL5 is involved in cellular processes such as membrane trafficking and signal transduction. OSBPL5 is a part of the larger OSBP-related protein complex which regulates lipid interactions and distribution in cells. It influences vesicular transport and is implicated in the modulation of signaling pathways that govern cell growth and development.
- Pathways
OSBPL5 functions in the phosphoinositide signaling and lipid metabolic pathways. It plays an important role in the transfer of lipids between the endoplasmic reticulum and other organelles impacting the regulation of phosphatidylinositol at the plasma membrane. OSBPL5 interacts with proteins like PI4KIIIα to regulate these processes. This interaction is important for maintaining cellular lipid dynamics necessary for various physiological functions.
- Associated diseases and disorders
OSBPL5's lipid transfer function associates with metabolic disorders like non-alcoholic fatty liver disease (NAFLD). Disruptions in lipid homeostasis by OSBPL5 can lead to excessive lipid accumulation in liver cells. Additionally OSBPL5 may have implications in neurological disorders given its expression in the brain alongside proteins such as APP that are involved in similar pathways. Understanding its role could provide insights into these diseases potentially aiding in therapeutic interventions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (ab127171)
ab127171, at 1/500 dilution, staining OSBPL5/ORP5 in paraffin-embedded breast cancer tissue by immunohistochemistry
Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (ab127171)
Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling OSBPL5/ORP5 using ab127171 at 1/500 dilution (green) in ICC/IF.
The nuclear counter stain is Hoechst 33342 (blue).
Western blot - Anti-OSBPL5/ORP5 antibody (ab127171)
7.5% SDS PAGE
All lanes: Western blot - Anti-OSBPL5/ORP5 antibody (ab127171) at 1/500 dilution
All lanes: HeLa whole cell lysate at 30 µg
Predicted band size: 95 kDa, 99 kDa
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