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AB127171

Anti-OSBPL5/ORP5 antibody

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(1 Publication)

Rabbit Polyclonal OSBPL5/ORP5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human OSBPL5 aa 200-500.

View Alternative Names

KIAA1534, OBPH1, ORP5, OSBPL5, Oxysterol-binding protein-related protein 5, ORP-5, OSBP-related protein 5, Oxysterol-binding protein homolog 1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (AB127171)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (AB127171)

ab127171, at 1/500 dilution, staining OSBPL5/ORP5 in paraffin-embedded breast cancer tissue by immunohistochemistry

Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (AB127171)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (AB127171)

Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling OSBPL5/ORP5 using ab127171 at 1/500 dilution (green) in ICC/IF.

The nuclear counter stain is Hoechst 33342 (blue).

Western blot - Anti-OSBPL5/ORP5 antibody (AB127171)
  • WB

Unknown

Western blot - Anti-OSBPL5/ORP5 antibody (AB127171)

7.5% SDS PAGE

All lanes:

Western blot - Anti-OSBPL5/ORP5 antibody (ab127171) at 1/500 dilution

All lanes:

HeLa whole cell lysate at 30 µg

Predicted band size: 95 kDa,99 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human OSBPL5 aa 200-500. The exact immunogen used to generate this antibody is proprietary information.

Q9H0X9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Alternatively Tris-EDTA buffer ( pH8.0) may be used.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OSBPL5 also known as ORP5 is a member of the oxysterol-binding protein (OSBP) family. This protein weighs approximately 99 kDa and is expressed in various tissues including the liver and the brain. OSBPL5 functions primarily as a lipid transfer protein playing a mechanical role in maintaining lipid homeostasis at membrane contact sites. It facilitates the transfer of phosphatidylserine and phosphatidylinositol-4-phosphate between membranes which is essential for cellular lipid regulation.
Biological function summary

The OSBP-like protein OSBPL5 is involved in cellular processes such as membrane trafficking and signal transduction. OSBPL5 is a part of the larger OSBP-related protein complex which regulates lipid interactions and distribution in cells. It influences vesicular transport and is implicated in the modulation of signaling pathways that govern cell growth and development.

Pathways

OSBPL5 functions in the phosphoinositide signaling and lipid metabolic pathways. It plays an important role in the transfer of lipids between the endoplasmic reticulum and other organelles impacting the regulation of phosphatidylinositol at the plasma membrane. OSBPL5 interacts with proteins like PI4KIIIα to regulate these processes. This interaction is important for maintaining cellular lipid dynamics necessary for various physiological functions.

OSBPL5's lipid transfer function associates with metabolic disorders like non-alcoholic fatty liver disease (NAFLD). Disruptions in lipid homeostasis by OSBPL5 can lead to excessive lipid accumulation in liver cells. Additionally OSBPL5 may have implications in neurological disorders given its expression in the brain alongside proteins such as APP that are involved in similar pathways. Understanding its role could provide insights into these diseases potentially aiding in therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane : specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed : 23934110, PubMed : 26206935). May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes (PubMed : 21220512). Binds 25-hydroxycholesterol and cholesterol (PubMed : 17428193).
See full target information OSBPL5
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of lipid research 57:1845-1853 PubMed27530118

2016

Oxysterol binding protein-related protein 8 mediates the cytotoxicity of 25-hydroxycholesterol.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwei Li,Xiuting Zheng,Ning Lou,Wenbin Zhong,Daoguang Yan
View all publications
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Product promise

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