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AB59016

Anti-OSBPL5/ORP5 antibody

1

(1 Review)

|

(7 Publications)

Goat Polyclonal OSBPL5/ORP5 antibody. Suitable for Flow Cyt, ICC/IF, IHC-P, WB and reacts with Human, Rat samples. Cited in 7 publications.

View Alternative Names

KIAA1534, OBPH1, ORP5, OSBPL5, Oxysterol-binding protein-related protein 5, ORP-5, OSBP-related protein 5, Oxysterol-binding protein homolog 1

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (AB59016)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (AB59016)

ab59016 (5µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (AB59016)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (AB59016)

ab59016 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (AB59016)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL5/ORP5 antibody (AB59016)

ab59016 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Flow Cytometry - Anti-OSBPL5/ORP5 antibody (AB59016)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-OSBPL5/ORP5 antibody (AB59016)

ab59016 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (AB59016)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-OSBPL5/ORP5 antibody (AB59016)

ab59016 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Western blot - Anti-OSBPL5/ORP5 antibody (AB59016)
  • WB

Supplier Data

Western blot - Anti-OSBPL5/ORP5 antibody (AB59016)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-OSBPL5/ORP5 antibody (ab59016) at 0.1 µg/mL

All lanes:

Jurkat cell lysate in RIPA buffer at 35 µg

Predicted band size: 99 kDa

Observed band size: 100 kDa

false

Western blot - Anti-OSBPL5/ORP5 antibody (AB59016)
  • WB

Supplier Data

Western blot - Anti-OSBPL5/ORP5 antibody (AB59016)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-OSBPL5/ORP5 antibody (ab59016) at 0.3 µg/mL

All lanes:

Rat Spleen lysate in RIPA buffer at 35 µg

Predicted band size: 99 kDa

Observed band size: 100 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is expected to recognise both reported isoforms (NP_065947.1; NP_663613.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.3 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.03-0.3 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OSBPL5 also known as ORP5 is a member of the oxysterol-binding protein (OSBP) family. This protein weighs approximately 99 kDa and is expressed in various tissues including the liver and the brain. OSBPL5 functions primarily as a lipid transfer protein playing a mechanical role in maintaining lipid homeostasis at membrane contact sites. It facilitates the transfer of phosphatidylserine and phosphatidylinositol-4-phosphate between membranes which is essential for cellular lipid regulation.
Biological function summary

The OSBP-like protein OSBPL5 is involved in cellular processes such as membrane trafficking and signal transduction. OSBPL5 is a part of the larger OSBP-related protein complex which regulates lipid interactions and distribution in cells. It influences vesicular transport and is implicated in the modulation of signaling pathways that govern cell growth and development.

Pathways

OSBPL5 functions in the phosphoinositide signaling and lipid metabolic pathways. It plays an important role in the transfer of lipids between the endoplasmic reticulum and other organelles impacting the regulation of phosphatidylinositol at the plasma membrane. OSBPL5 interacts with proteins like PI4KIIIα to regulate these processes. This interaction is important for maintaining cellular lipid dynamics necessary for various physiological functions.

OSBPL5's lipid transfer function associates with metabolic disorders like non-alcoholic fatty liver disease (NAFLD). Disruptions in lipid homeostasis by OSBPL5 can lead to excessive lipid accumulation in liver cells. Additionally OSBPL5 may have implications in neurological disorders given its expression in the brain alongside proteins such as APP that are involved in similar pathways. Understanding its role could provide insights into these diseases potentially aiding in therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lipid transporter involved in lipid countertransport between the endoplasmic reticulum and the plasma membrane : specifically exchanges phosphatidylserine with phosphatidylinositol 4-phosphate (PI4P), delivering phosphatidylserine to the plasma membrane in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds phosphatidylserine and PI4P in a mutually exclusive manner (PubMed : 23934110, PubMed : 26206935). May cooperate with NPC1 to mediate the exit of cholesterol from endosomes/lysosomes (PubMed : 21220512). Binds 25-hydroxycholesterol and cholesterol (PubMed : 17428193).
See full target information OSBPL5
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Cell stress & chaperones 28:395-407 PubMed37314629

2023

ORP5 promotes migration and invasion of cervical cancer cells by inhibiting endoplasmic reticulum stress.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Wu,Luyao Min,Peng Zhang,Lin Zhang,Yuting Xu,Danhua Li,Maojin Zheng,Dongsheng Pei,Qingling Wang

Cell death discovery 8:219 PubMed35449154

2022

ORP5 promotes tumor metastasis via stabilizing c-Met in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Li Song,Lin Zhang,Yun Zhou,Xiaotong Shao,Yuting Xu,Dongsheng Pei,Qingling Wang

BMC pulmonary medicine 22:30 PubMed35000595

2022

The LMCD1-AS1/miR-526b-3p/OSBPL5 axis promotes cell proliferation, migration and invasion in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Hu,Yankai Yu,Haining Wang

Journal of immunology (Baltimore, Md. : 1950) 204:1134-1145 PubMed31953353

2020

OSBP-Related Protein 5L Maintains Intracellular IP/Ca Signaling and Proliferation in T Cells by Facilitating PIP Hydrolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyang Xu,Biying Zhu,Xiuye Cao,Shannai Li,Dan Li,Huihao Zhou,Vesa M Olkkonen,Wenbin Zhong,Jun Xu,Daoguang Yan

The Journal of cell biology 217:1797-1813 PubMed29472386

2018

PI(4,5)P controls plasma membrane PI4P and PS levels via ORP5/8 recruitment to ER-PM contact sites.

Applications

Unspecified application

Species

Unspecified reactive species

Mira Sohn,Marek Korzeniowski,James P Zewe,Rachel C Wills,Gerald R V Hammond,Jana Humpolickova,Lukas Vrzal,Dominika Chalupska,Vaclav Veverka,Gregory D Fairn,Evzen Boura,Tamas Balla

The Journal of biological chemistry 293:3806-3818 PubMed29358326

2018

Oxysterol-binding protein-related protein 5 (ORP5) promotes cell proliferation by activation of mTORC1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ximing Du,Armella Zadoorian,Ivan E Lukmantara,Yanfei Qi,Andrew J Brown,Hongyuan Yang

Nature communications 8:757 PubMed28970484

2017

ORP5 and ORP8 bind phosphatidylinositol-4, 5-biphosphate (PtdIns(4,5)P ) and regulate its level at the plasma membrane.

Applications

Unspecified application

Species

Unspecified reactive species

Rajesh Ghai,Ximing Du,Huan Wang,Jiangqing Dong,Charles Ferguson,Andrew J Brown,Robert G Parton,Jia-Wei Wu,Hongyuan Yang
View all publications
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