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AB151691

Anti-OSBPL9 antibody [EPR9333]

4

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal OSBPL9 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

ORP9, OSBP4, OSBPL9, Oxysterol-binding protein-related protein 9, ORP-9, OSBP-related protein 9

3 Images
Flow Cytometry (Intracellular) - Anti-OSBPL9 antibody [EPR9333] (AB151691)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-OSBPL9 antibody [EPR9333] (AB151691)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling OSBPL9 with ab151691 at 1/10 dilution (red) compared to a nonspecific control antibody (green).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL9 antibody [EPR9333] (AB151691)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSBPL9 antibody [EPR9333] (AB151691)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling OSBPL9 with ab151691 at 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-OSBPL9 antibody [EPR9333] (AB151691)
  • WB

Unknown

Western blot - Anti-OSBPL9 antibody [EPR9333] (AB151691)

All lanes:

Western blot - Anti-OSBPL9 antibody [EPR9333] (ab151691) at 1/1000 dilution

Lane 1:

Human fetal brain tissue lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 83 kDa

false

  • Carrier free

    Anti-OSBPL9 antibody [EPR9333] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9333

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

AB126754

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RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UCKL1 antibody [EPR7384] (AB126754)

  • 0
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Primary Antibodies

AB75973

Anti-Ferritin heavy chain antibody [EPR3004Y]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Ferritin heavy chain antibody [EPR3004Y] (AB75973)

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Primary Antibodies

AB69090

Anti-Ferritin Light Chain antibody

Western blot - Anti-Ferritin Light Chain antibody (AB69090)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OSBPL9 also referred to as oxysterol-binding protein-like 9 is a member of the oxysterol-binding protein (OSBP)-related protein family. This protein family involves intracellular lipid transport. OSBPL9 has a molecular weight of approximately 89 kDa. It is expressed in various tissues with high levels in brain pancreas and intestine. The protein possesses a pleckstrin homology domain and a conserved OSBP-related domain which play roles in binding to lipids and facilitating their transport within cells.
Biological function summary

OSBPL9 participates in lipid metabolism and cellular lipid homeostasis. It binds to phosphoinositides and sterols regulating lipid distribution and signaling. Studies indicate that OSBPL9 forms part of a complex involved in the transfer of lipids at the membrane contact sites between organelles such as the endoplasmic reticulum and the Golgi apparatus. These interactions highlight its integral role in maintaining lipid equilibrium across cellular compartments.

Pathways

A deeper understanding of OSBPL9 shows its involvement in signaling pathways alongside other lipid-binding proteins. It plays a significant role within the sterol regulatory element-binding protein (SREBP) signaling pathway and potentially the PI3K-Akt signaling pathway. These pathways are essential in lipid synthesis and cellular growth. OSBPL9 shares functional interactions with proteins like OSBP and other OSBP-related proteins emphasizing its collaborative involvement in these pathways.

OSBPL9 association with dyslipidemia and metabolic disorders has been observed due to its influence on lipid homeostasis. Alterations in OSBPL9 function may contribute to abnormal lipid levels impacting conditions like atherosclerosis. The protein's connection to metabolic pathways suggests a linkage with proteins such as OSBPL10 further indicating its potential involvement in the pathogenesis of these metabolic disorders. Researchers continue to study OSBPL9 to better understand its role in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Interacts with OSBPL11 to function as lipid transfer proteins (PubMed : 39106189). Together they form a heterodimer that localizes at the ER-trans-Golgi membrane contact sites, and exchanges phosphatidylserine (1,2-diacyl-sn-glycero-3-phospho-L-serine, PS) for phosphatidylinositol-4-phosphate (1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate), PI(4)P) between the two organelles, a step that is critical for sphingomyelin synthesis in the Golgi complex (PubMed : 39106189).
See full target information OSBPL9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

eLife 8: PubMed31294692

2019

In vivo identification of GTPase interactors by mitochondrial relocalization and proximity biotinylation.

Applications

Unspecified application

Species

Unspecified reactive species

Alison K Gillingham,Jessie Bertram,Farida Begum,Sean Munro
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com