Anti-OSMR antibody [EPR24611-71]

• WB

Lab

Western blot - Anti-OSMR antibody [EPR24611-71] (AB282577)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression pattern & observed MW are consistent with what has been described in the literature (PMID : 8999038, 9188471, 21394648).

Negative control : JAR, Jurkat (PMID : 8999038, 8119965, 28607076).

Exposure time : 5.5 seconds

All lanes:

Western blot - Anti-OSMR antibody [EPR24611-71] (ab282577) at 1/1000 dilution

Lane 1:

A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

JAR (human placenta choriocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 111 kDa

Observed band size: 150-180 kDa,50 kDa

false

• WB

Lab

Western blot - Anti-OSMR antibody [EPR24611-71] (AB282577)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

OSMR is a glycoprotein and can be de-glycosylated by PNGase F. The molecular mass observed is consistent with the literature (PMID : 8999038, 9188471, 22056139).

Exposure time : 59 seconds

All lanes:

Western blot - Anti-OSMR antibody [EPR24611-71] (ab282577) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa whole cell lysate treated with PNGase F at 15 µg

Lane 3:

A375 (human malignant melanoma epithelial cell) whole cell lysate at 15 µg

Lane 4:

A375 whole cell lysate treated with PNGase F at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 111 kDa

Observed band size: 150-180 kDa,50 kDa

false

• WB

Lab

Western blot - Anti-OSMR antibody [EPR24611-71] (AB282577)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression pattern & observed MW are consistent with what has been described in the literature (PMID : 8999038).

Low expression : human tonsil (PMID : 8999038).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-OSMR antibody [EPR24611-71] (ab282577) at 1/1000 dilution

Lane 1:

Human placenta tissue lysate at 20 µg

Lane 2:

Human tonsil tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/2000 dilution

Predicted band size: 111 kDa

Observed band size: 150-180 kDa

false