AB273103

Anti-OSMR antibody [EPR24786-50]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal OSMR antibody. Suitable for WB and reacts with Mouse samples.

View Alternative Names

Osmrb, Osmr, Oncostatin-M-specific receptor subunit beta, Interleukin-31 receptor subunit beta, IL-31 receptor subunit beta, IL-31R subunit beta, IL-31R-beta, IL-31RB

3 Images

Lab

Western blot - Anti-OSMR antibody [EPR24786-50] (AB273103)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTOSMR is a glycosylated protein and can be deglycosylated by PNGase F.

The observed MW are consistent with what has been described in the literature (PMID : 26311783).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-OSMR antibody [EPR24786-50] (ab273103) at 1/1000 dilution

Lane 1:

Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 2:

3T3-L1 whole cell lysate treated with PNGase F at 15 µg

Lane 3:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 4:

NIH/3T3 whole cell lysate treated with PNGase F at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 111 kDa

false

Lab

Western blot - Anti-OSMR antibody [EPR24786-50] (AB273103)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed MW are consistent with what has been described in the literature (PMID : 27325693).

Negative control : M1, EL4 (PMID : 9920829).

Exposure time : 15 seconds.

All lanes:

Western blot - Anti-OSMR antibody [EPR24786-50] (ab273103) at 1/1000 dilution

Lane 1:

3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 3:

M1 (mouse myeloid leukemia myeloblast) whole cell lysate at 20 µg

Lane 4:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 111 kDa

false

Lab

Western blot - Anti-OSMR antibody [EPR24786-50] (AB273103)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTThe observed MW are consistent with what has been described in the literature (PMID : 26311783).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-OSMR antibody [EPR24786-50] (ab273103) at 1/1000 dilution

All lanes:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 111 kDa

false

Carrier free

Anti-OSMR antibody [EPR24786-50] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24786-50

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

- WB suitable for mouse only - FC and IP not suitable for human and mouse - ICC not suitable for human - IHC not suitable for human, mouse and rat

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Oncostatin M receptor commonly known as OSMR is an important membrane-bound receptor involved in numerous cellular processes. It is part of the type I cytokine receptor family and often forms heterodimers with gp130 to mediate its functions. OSMR has a mass of approximately 115 kDa and predominantly expresses in tissues such as skin lung liver and the nervous system. Alternative names for OSMR include Oncostatin-M-specific receptor beta subunit and IL-31 receptor beta.

Biological function summary

OSMR plays a significant role in regulating inflammatory responses and cell growth. It is part of a receptor complex that upon activation triggers intracellular signaling cascades driven by cytokines like Oncostatin M and IL-31. OSMR involvement influences processes including acute phase responses hematopoiesis and nerve cell differentiation. Its action helps mediate the communication between cells to respond to external inflammatory signals effectively.

Pathways

OSMR is actively involved in the JAK/STAT signaling pathway and the MAPK pathway. In JAK/STAT OSMR collaborates with related proteins like gp130 and participates in promoting transcriptional activity of specific genes in response to cytokine signaling. OSMR’s engagement in the MAPK pathway influences cellular responses such as proliferation differentiation and apoptosis linking it with proteins such as MAP Kinases that further proceed this signaling cascade.

OSMR shows a connection with atopic dermatitis and various cancers. Aberrant OSMR signaling can lead to excessive inflammatory responses contributing to skin conditions like atopic dermatitis often involving IL-31 in the pathway. In cancer elevated OSMR expression correlates with tumorigenesis and progression establishing its interaction with oncogenic proteins in the STAT3 pathway which may promote malignant cell behavior. Through these disease interactions OSMR represents a potential therapeutic target worth exploring.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Associates with IL31RA to form the IL31 receptor (PubMed : 9920829). Binds IL31 to activate STAT3 and possibly STAT1 and STAT5 (By similarity). Capable of transducing OSM-specific signaling events (By similarity).

See full target information Osmr

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OSMR antibody [EPR24786-50]

Western blot - Anti-OSMR antibody [EPR24786-50] (AB273103)

Western blot - Anti-OSMR antibody [EPR24786-50] (AB273103)

Western blot - Anti-OSMR antibody [EPR24786-50] (AB273103)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise