Anti-OSMR antibody [EPR24786-50] - BSA and Azide free

• WB

Lab

Western blot - Anti-OSMR antibody [EPR24786-50] - BSA and Azide free (AB284413)

This data was developed using ab273103, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

OSMR is a glycosylated protein and can be deglycosylated by PNGase F.

The observed MW are consistent with what has been described in the literature (PMID : 26311783).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-OSMR antibody [EPR24786-50] (<a href='/en-us/products/primary-antibodies/osmr-antibody-epr24786-50-ab273103'>ab273103</a>) at 1/1000 dilution

Lane 1:

Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 2:

3T3-L1 whole cell lysate treated with PNGase F at 15 µg

Lane 3:

Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 15 µg

Lane 4:

NIH/3T3 whole cell lysate treated with PNGase F at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 111 kDa

false

• WB

Lab

Western blot - Anti-OSMR antibody [EPR24786-50] - BSA and Azide free (AB284413)

This data was developed using ab273103, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed MW are consistent with what has been described in the literature (PMID : 27325693).

Negative control : M1, EL4 (PMID : 9920829).

Exposure time : 15 seconds.

All lanes:

Western blot - Anti-OSMR antibody [EPR24786-50] (<a href='/en-us/products/primary-antibodies/osmr-antibody-epr24786-50-ab273103'>ab273103</a>) at 1/1000 dilution

Lane 1:

3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 3:

M1 (mouse myeloid leukemia myeloblast) whole cell lysate at 20 µg

Lane 4:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 111 kDa

false

• WB

Lab

Western blot - Anti-OSMR antibody [EPR24786-50] - BSA and Azide free (AB284413)

This data was developed using ab273103, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The observed MW are consistent with what has been described in the literature (PMID : 26311783).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-OSMR antibody [EPR24786-50] (<a href='/en-us/products/primary-antibodies/osmr-antibody-epr24786-50-ab273103'>ab273103</a>) at 1/1000 dilution

All lanes:

Mouse lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 111 kDa

false