Anti-OSMR antibody [EPR28222-64]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR28222-64] (AB315388)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling OSMR with ab315388 at 1/50 (9.56 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing mainly membranous staining in 3T3-L1 cell line.
Negative control : EL4 (PMID : 9920829)
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt

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Flow Cytometry - Anti-OSMR antibody [EPR28222-64] (AB315388)

Flow cytometric analysis of M1 (mouse myeloid leukemia myeloblast, Left) / 3T3-L1 (mouse embryonic fibroblast, Right) cells labelling OSMR with ab315388 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : M1. Gated on viable cells.

• Flow Cyt

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Flow Cytometry - Anti-OSMR antibody [EPR28222-64] (AB315388)

Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / NIH/3T3 (mouse embryonic fibroblast, Right) cells labelling OSMR with ab315388 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Negative control : EL4. Gated on viable cells.

• WB

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Western blot - Anti-OSMR antibody [EPR28222-64] (AB315388)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

OMSR is a glycoprotein of approximately 200-250 kDa and detected as a 111kDa band after treated with Protein Deglycosylation MIX II.

All lanes:

Western blot - Anti-OSMR antibody [EPR28222-64] (ab315388) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate treated with Protein Deglycosylation Mix II at 15 µg

Lane 2:

Untreated NIH/3T3 whole cell lysate at 15 µg

Lane 3:

Mouse colon tissue lysate at 20 µg

Lane 4:

Mouse skin tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 200-250 kDa

false

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-OSMR antibody [EPR28222-64] (AB315388)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-OSMR antibody [EPR28222-64] (ab315388) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 3:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

Lane 4:

M1 (mouse myeloid leukemia myeloblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 200-250 kDa,36 kDa

false

Exposure time: 180s