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Rabbit Recombinant Monoclonal OSMR antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt and reacts with Mouse samples.

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Images

Western blot - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (AB315389), expandable thumbnail
  • Western blot - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (AB315389), expandable thumbnail
  • Flow Cytometry - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (AB315389), expandable thumbnail
  • Flow Cytometry - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (AB315389), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (AB315389), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBFlow CytIHC-PIP
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Associates with IL31RA to form the IL31 receptor (PubMed:9920829). Binds IL31 to activate STAT3 and possibly STAT1 and STAT5 (By similarity). Capable of transducing OSM-specific signaling events (By similarity).

Alternative names

Osmrb, Osmr, Oncostatin-M-specific receptor subunit beta, Interleukin-31 receptor subunit beta, IL-31 receptor subunit beta, IL-31R subunit beta, IL-31R-beta, IL-31RB

Recommended products

Rabbit Recombinant Monoclonal OSMR antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt and reacts with Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR28222-64
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab315389 is the carrier-free version of Anti-OSMR antibody [EPR28222-64] ab315388.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Oncostatin M receptor commonly known as OSMR is an important membrane-bound receptor involved in numerous cellular processes. It is part of the type I cytokine receptor family and often forms heterodimers with gp130 to mediate its functions. OSMR has a mass of approximately 115 kDa and predominantly expresses in tissues such as skin lung liver and the nervous system. Alternative names for OSMR include Oncostatin-M-specific receptor beta subunit and IL-31 receptor beta.

Biological function summary

OSMR plays a significant role in regulating inflammatory responses and cell growth. It is part of a receptor complex that upon activation triggers intracellular signaling cascades driven by cytokines like Oncostatin M and IL-31. OSMR involvement influences processes including acute phase responses hematopoiesis and nerve cell differentiation. Its action helps mediate the communication between cells to respond to external inflammatory signals effectively.

Pathways

OSMR is actively involved in the JAK/STAT signaling pathway and the MAPK pathway. In JAK/STAT OSMR collaborates with related proteins like gp130 and participates in promoting transcriptional activity of specific genes in response to cytokine signaling. OSMR’s engagement in the MAPK pathway influences cellular responses such as proliferation differentiation and apoptosis linking it with proteins such as MAP Kinases that further proceed this signaling cascade.

Associated diseases and disorders

OSMR shows a connection with atopic dermatitis and various cancers. Aberrant OSMR signaling can lead to excessive inflammatory responses contributing to skin conditions like atopic dermatitis often involving IL-31 in the pathway. In cancer elevated OSMR expression correlates with tumorigenesis and progression establishing its interaction with oncogenic proteins in the STAT3 pathway which may promote malignant cell behavior. Through these disease interactions OSMR represents a potential therapeutic target worth exploring.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (ab315389), expandable thumbnail

    Western blot - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (ab315389)

    This data was developed using Anti-OSMR antibody [EPR28222-64] ab315388, the same antibody clone in a different buffer formulation.

    OMSR is a glycoprotein of approximately 200-250 kDa and detected as a 111kDa band after treated with Protein Deglycosylation MIX II.

    All lanes: Western blot - Anti-OSMR antibody [EPR28222-64] (Anti-OSMR antibody [EPR28222-64] ab315388) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate treated with Protein Deglycosylation Mix II at 15 µg with 5% NFDM/TBST

    Lane 2: Untreated NIH/3T3 whole cell lysate at 15 µg with 5% NFDM/TBST

    Lane 3: Mouse colon tissue lysate at 20 µg with 5% NFDM/TBST

    Lane 4: Mouse skin tissue lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 200-250 kDa

    Exposure time: 70s

  • Western blot - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (ab315389), expandable thumbnail

    Western blot - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (ab315389)

    This data was developed using Anti-OSMR antibody [EPR28222-64] ab315388, the same antibody clone in a different buffer formulation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-OSMR antibody [EPR28222-64] (Anti-OSMR antibody [EPR28222-64] ab315388) at 1/1000 dilution

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 4: M1 (mouse myeloid leukemia myeloblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 200-250 kDa, 36 kDa

    Exposure time: 180s

  • Flow Cytometry - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (ab315389), expandable thumbnail

    Flow Cytometry - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (ab315389)

    This data was developed using Anti-OSMR antibody [EPR28222-64] ab315388, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of M1 (mouse myeloid leukemia myeloblast, Left) / 3T3-L1 (mouse embryonic fibroblast, Right) cells labelling OSMR with Anti-OSMR antibody [EPR28222-64] ab315388 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: M1. Gated on viable cells.

  • Flow Cytometry - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (ab315389), expandable thumbnail

    Flow Cytometry - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (ab315389)

    This data was developed using Anti-OSMR antibody [EPR28222-64] ab315388, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of EL4 (mouse lymphoma T lymphocyte, Left) / NIH/3T3 (mouse embryonic fibroblast, Right) cells labelling OSMR with Anti-OSMR antibody [EPR28222-64] ab315388 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Negative control: EL4. Gated on viable cells.

  • Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (ab315389), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR28222-64] - BSA and Azide free (ab315389)

    This data was developed using Anti-OSMR antibody [EPR28222-64] ab315388, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 3T3-L1 (mouse embryonic fibroblast) cells labelling OSMR with Anti-OSMR antibody [EPR28222-64] ab315388 at 1/50 (9.56 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing mainly membranous staining in 3T3-L1 cell line.

    Negative control: EL4 (PMID: 9920829)

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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