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Rabbit Recombinant Monoclonal OSMR antibody. Suitable for ICC/IF and reacts with Transfected cell line - Human, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR29920-534] (AB323715), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR29920-534] (AB323715), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR29920-534] (AB323715), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-PWB
Human
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell line - Human
Dilution info
1/100
Notes

-

Species
Human
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Transfected cell line - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Transfected cell line - Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Associates with IL31RA to form the IL31 receptor. Binds IL31 to activate STAT3 and possibly STAT1 and STAT5. Capable of transducing OSM-specific signaling events.

Alternative names

OSMRB, OSMR, Oncostatin-M-specific receptor subunit beta, Interleukin-31 receptor subunit beta, IL-31 receptor subunit beta, IL-31R subunit beta, IL-31R-beta, IL-31RB

Rabbit Recombinant Monoclonal OSMR antibody. Suitable for ICC/IF and reacts with Transfected cell line - Human, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR29920-534
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR29920-534] (ab323715), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR29920-534] (ab323715)

    OSMR Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-OSMR antibody

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling OSMR with ab323715 at 1/100 (5.08 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in 293T cells (shown in green) transfected with a human OSMR expression vector containing a Myc tag. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-Myc tag antibody [9E10] ab223894 Anti-Myc tag mouse monoclonal antibody (Alexa Fluor® 594) was used to counterstain tubulin at 1/100 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR29920-534] (ab323715), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR29920-534] (ab323715)

    OSMR Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-OSMR antibody

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A375 (human malignant melanoma epithelial cell) cells labelling OSMR with ab323715 at 1/100 (5.08 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing membrane and cytoplasmic staining in A375 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Low expression: Jurkat (PMID: 28607076).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR29920-534] (ab323715), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-OSMR antibody [EPR29920-534] (ab323715)

    OSMR Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-OSMR antibody

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized A-172 (human brain glioblastoma cell) cells labelling OSMR with ab323715 at 1/100 (5.08 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing membrane and cytoplasmic staining in A-172 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Low expression: JAR (PMID: 8999038).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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