Anti-Osteocalcin antibody

• WB

Supplier Data

Western blot - Anti-Osteocalcin antibody (AB93876)

Western blot analysis of mouse lung tissue lysate with anti-osteocalcin antibody.

All lanes:

Western blot - Anti-Osteocalcin antibody (ab93876) at 1/100 dilution

All lanes:

Mouse lung tissue lysate

Predicted band size: 11 kDa

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• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteocalcin antibody (AB93876)

Immunohistochemical staining of formalin-fixed paraffin-embedded liver carcinoma staining with anti-osteocalcin antibody at a dilution of 1/100.

• IHC-Fr

CiteAb

Immunohistochemistry (Frozen sections) - Anti-Osteocalcin antibody (AB93876)

Osteocalcin immunohistochemistry-immunofluorescence using Anti-Osteocalcin antibody ab93876. Publication image and figure legend from Wang, K., Wang, Y., et al., 2020, Cell Death Dis, Pubmed 32427900.

The effect of bone-targeted OGRU on the function of osteoblasts in HLU mice.a Representative images of immunofluorescence staining and H&E staining of same sections in the femurs of mice. The pcDNA3.1(+)-OGRU was labeled with GFP (green, left column). Immunofluorescence staining of Ocn was performed to detect osteoblasts (red, middle-left column). Merged images with DAPI staining showed co-staining of pcDNA3.1(+)-OGRU and osteoblasts. H&E staining was included in the right column. Scale bar = 20 μm (n = 5). b Schematic of the experimental design. c Representative images of osteocalcin immunohistochemical staining in the femurs of mice in the indicated groups (n = 5). Scale bars : 50 μm. d Representative images of Goldner's trichrome staining of the trabecular bone at distal femurs of mice in the indicated groups (n = 5). Osteoid stains are shown in red (indicated by the black arrows), and mineralized bone stains in green. Scale bars : 50 µm. e Representative images of calcein double labeling (n = 5). Scale bars : 10 μm. All data are the mean ± SD. *p < < 0.05, **p < < 0.01. NS not significant.

• IHC

CiteAb

Immunohistochemistry - Anti-Osteocalcin antibody (AB93876)

Immunohistochemistry-immunofluorescence using Anti-Osteocalcin antibody, ab93876. Publication image from Wang, L. et al., 2016, Nat Commun, 27966526. Legend direct from paper.

Alteration of mTORC1 activity in osteoblasts affects angiogenesis in mouse bone.(a) Representative images of immunostaining of pS6 (Ser235/236) and osteocalcin (Ocn) in 12-week-old male mice bone. Scale bar, 50 µm. (b) Photograph of hindlimbs of 6-week-old male δTsc1 (δT) and δRaptor (δR) mice and their littermate controls (Ctrl). Scale bar, 1 cm. (c) Representative images of CD31+EMCN+ microvessels and quantitative analysis of type H microvessel density in femur sections of 12-week-old male mice. Scale bar, 100 µm. n=9 per group. (d) Consistent numbers of CD31+ vessels in surrounding muscle of mouse bone. Scale bar, 100 µm. Data are shown as mean±s.d. n=9 per group. Data are shown as mean±s.d. *P<0.05, **P<0.01 (Student's t-test). For all panels in this figure, data are representative for three independent experiments.

• IHC

CiteAb

Immunohistochemistry - Anti-Osteocalcin antibody (AB93876)

Immunohistochemistry-immunofluorescence using Anti-Osteocalcin antibody, ab93876. Publication image from Wang, L. et al., 2016, Nat Commun, 27966526. Legend direct from paper.

Regulation of VEGF in osteoblasts by mTORC1 does not explain the vascular phenotypes of mutant mouse bone.(a) Representative images of immunostaining of VEGF and Ocn in 12-week-old male mice bone and quantitative analysis of VEGF+ osteoblasts compared with total osteoblasts. Scale bar, 50 µm. n=9 per group. (b) Representative images of immunostaining of CD31, KDR and pKDR (Y1175) in 12-week-old male mice bone, and quantitative analysis of KDR+ and pKDR+ ECs compared with total ECs in bone marrow. Scale bar, 50 µm. (c) Western blot of VEGF in primary osteoblasts. (d) Western blot of phosphorylation of KDR, PLCγ1 and ERK1/2 in HUVECs treated with CM from primary osteoblasts for 10 min. Data are shown as mean±s.d. *P<0.05, **P<0.01 (Student's t-test). Ctrl, control.

• WB

CiteAb

Western blot - Anti-Osteocalcin antibody (AB93876)

Western Blotting using Anti-Osteocalcin antibody, ab93876. Publication image from Wang, L. et al., 2016, Nat Commun, 27966526. Legend direct from paper.

Cxcl9 suppresses osteogenesis by interacting with VEGF and abrogating binding of VEGF to osteoblasts.(a) BrdU staining of MC3T3-E1 cells and quantitative analysis of BrdU+ cells out of total cells. Scale bar, 100 µm. (b) Western blot analysis of osteoblastic marker Ocn and Runx2 expression in MC3T3-E1 cells on the seventh day of osteogenic induction. (c) Alizarin red staining of differentiated MC3T3-E1 cells on the fourteenth day. Scale bar, 1 cm. (d) Binding of 125I–VEGF164 to MC3T3-E1 cells in the presence of increasing concentrations of Cxcl9. Shown is the specific binding, which was calculated by subtracting the nonspecific binding from the total binding. Data are shown as mean±s.d. *P<0.05, **P<0.01 (Student's t-test).

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• WB

CiteAb

Western blot - Anti-Osteocalcin antibody (AB93876)

Osteocalcin western blotting using Anti-Osteocalcin antibody ab93876. Publication image and figure legend from Yang, K., Cao, F., et al., 2022, Front Pharmacol, Pubmed 35387349.

Metformin promotes osteogenic differentiation of MC3T3-E1 cells. (A) Cell viability after treatment with different concentrations of metformin (0.1, 0.2 and 0.3 mM). (B) ALP activity was detected to explicit the osteogenic effect of metformin. (C) The mRNA level of COLL-1, OCN and Runx2 after osteogenic induction and treatment with metformin. (D) The mineralization of osteoblast demonstrated by Alizarin red S staining was most obvious after treatment with 0.2 mM metformin. (E) The protein level of Collagen I, OCN and Runx2 under different concentrations of metformin. (F) Relative protein expression level of the proteins in (E) compared with control group. Experiments were implemented in triplicate. Data are means ± SD, *p < < 0.05, **p < < 0.01 compared with control cells and #p < < 0.05, ##p < < 0.01 compared with osteogenic induction alone analyzed by using ANOVA.

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• WB

CiteAb

Western blot - Anti-Osteocalcin antibody (AB93876)

Osteocalcin western blotting using Anti-Osteocalcin antibody ab93876. Publication image and figure legend from Xu, X. Y., He, X. T., et al., 2019, Cell Death Dis, Pubmed 30622236.

Osteogenesis of Ad-P2X7 (MOI = 20)-transfected PDLSCs under normal or inflammatory conditions with or without the addition of a specific P2X7R antagonist (BBG) or specific inhibitors of PI3K (BKM 120), AKT (MK 2206), or mTOR (Rapamycin).a ALP staining (scale bar = 1 mm) and the quantitative result. b Alizarin red staining (scale bar = 500 μm) and the quantitative result of Ad-P2X7-transfected PDLSCs. western blotting analysis of c Runx2 and d OCN expression in Ad-P2X7-transfected PDLSCs. Data are presented as the mean ± S.D. for n = 4; ns indicates no significant difference between the indicated columns; *p < < 0.05, **p < < 0.01, and ***p < < 0.001 indicate significant differences between the indicated columns

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