AB133612

Anti-Osteocalcin antibody [EPR3690]

RabMAb
Recombinant
What is this?

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(71 Publications)

Rabbit Recombinant Monoclonal Osteocalcin antibody. Suitable for WB and reacts with Human, Recombinant full length protein - Human samples. Cited in 71 publications.

View Alternative Names

Osteocalcin, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein, BGP, BGLAP

3 Images

Lab

Western blot - Anti-Osteocalcin antibody [EPR3690] (AB133612)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-Osteocalcin antibody [EPR3690] (ab133612) at 1/1000 dilution

All lanes:

Human articular cartilage tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/1000 dilution

Predicted band size: 11 kDa

Observed band size: 19 kDa

true

Exposure time: 158s

Unknown

Western blot - Anti-Osteocalcin antibody [EPR3690] (AB133612)

All lanes:

Western blot - Anti-Osteocalcin antibody [EPR3690] (ab133612) at 1/1000 dilution

All lanes:

Human Osteocalcin recombinant protein at 0.01 µg

Predicted band size: 11 kDa

false

Unknown

Western blot - Anti-Osteocalcin antibody [EPR3690] (AB133612)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Osteocalcin antibody [EPR3690] (ab133612) at 1.2 µg/mL

All lanes:

Human osteocalcin recombinant protein at 0.015 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 11 kDa

false

Carrier free

Anti-Osteocalcin antibody [EPR3690] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3690

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody fails to detect endogenous Osteocalcin in cell lines and tissues tested. So we don`t guarantee WB in natural samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteocalcin also known as bone gamma-carboxyglutamic acid-containing protein (BGLAP) is a small non-collagenous protein with a molecular weight of approximately 5.8 kDa. This protein is mainly expressed in osteoblasts which are bone-forming cells. It is an important component of the bone matrix and is involved in bone mineralization. Osteocalcin binds to calcium and incorporates into the hydroxyapatite crystals in the bone helping stabilize the bone structure. Researchers often study this protein as a marker for bone formation and turnover.

Biological function summary

Osteocalcin plays a significant role in regulating glucose metabolism and fat deposition. It acts as a hormone from the bone and influences energy metabolism and insulin sensitivity. Osteocalcin undergoes carboxylation a post-translational modification necessary for its function in bone. Beyond its structural role demineralized osteocalcin has been found to impact pancreatic β-cell function adipose tissue and male fertility although the pathways in these processes are not entirely understood yet.

Pathways

Osteocalcin is associated with the energy metabolism and bone remodeling pathways. It interacts with the insulin signaling pathway influencing insulin secretion by pancreatic β-cells and boosting insulin sensitivity. This interaction involves proteins like insulin receptor substrate 1 (IRS1) and glucose transporter type 4 (GLUT4) which play roles in glucose uptake. Osteocalcin also connects to bone remodeling pathways by influencing osteoclast differentiation and activity through osteoprotegrin and receptor activator of nuclear factor kappa-B ligand (RANKL).

Dysregulation of osteocalcin levels associates with metabolic syndrome and osteoporosis. It is linked with insulin resistance affecting glucose homeostasis and potentially contributing to diabetes development. Osteocalcin's involvement in bone density maintenance also makes it relevant in osteoporosis where bone formation balance is disrupted. The receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegrin which osteocalcin interacts with are other proteins connected to the pathogenesis of these conditions. Understanding osteocalcin's function helps in exploring therapeutic targets for these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Bone protein that constitutes 1-2% of the total bone protein, and which acts as a negative regulator of bone formation (PubMed : 3019668, PubMed : 6967872). Functions to limit bone formation without impairing bone resorption or mineralization (By similarity). It binds strongly to apatite and calcium (PubMed : 6967872).. The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes : acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development : osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development.

See full target information BGLAP

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Publications (71)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 18:820 PubMed37915040

2023

Carbamazepine regulates USP10 through miR-20a-5p to affect the deubiquitination of SKP2 and inhibit osteogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Wang,Ziye Liu,Dawei Niu,Haobo Li,Yaguang Han,Jinhui Peng,Qirong Qian

Experimental and therapeutic medicine 26:531 PubMed37869648

2023

FRZB affects aureus‑induced osteomyelitis in human bone marrow derived stem cells by regulating the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Wenyong Pang,Hongsong Fan,Hao Wang,Leibing Zhang

Journal of periodontal research 58:1248-1260 PubMed37767803

2023

Hypoxia dissociates HDAC6/FOXO1 complex and aggregates them into nucleus to regulate autophagy and osteogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yixin Xu,Yixin Wang,Hui Xiao,Yongming Li

iScience 26:107923 PubMed37810257

2023

Circ_0000888 regulates osteogenic differentiation of periosteal mesenchymal stem cells in congenital pseudarthrosis of the tibia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoyang Li,Yaoxi Liu,Yiyong Huang,Qian Tan,Haibo Mei,Guanghui Zhu,Kun Liu,Ge Yang

Chemical biology & drug design 102:1489-1505 PubMed37690812

2023

Exploring the mechanism of Astragali radix for promoting osteogenic differentiation based on network pharmacology, molecular docking, and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Zenghui Tian,Yingying Li,Xiaoying Wang,Kaiying Cui,Jinxing Guo,Mingliang Wang,Yanke Hao,Farong Zhang

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 38:1480-1496 PubMed37537994

2023

GSTT1 as a Predictive Marker and Enhancer for Osteogenic Potential of Human Adipose-Derived Stromal/Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Eugene Lee,Jae-Yeon Moon,Ji-Yun Ko,Seo-Young Park,Gun-Il Im

Journal of orthopaedic surgery and research 18:546 PubMed37516879

2023

Exosomes promote hFOB1.19 proliferation and differentiation via LINC00520.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Wu,Licheng Zhang,Hui Liu,Jinhui Zhang,Peifu Tang

Journal of nanobiotechnology 21:193 PubMed37316836

2023

Spontaneous formation of MXene-oxidized sono/chemo-dynamic sonosensitizer/nanocatalyst for antibacteria and bone-tissue regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yu,Houyi Sun,Qunshan Lu,Junyuan Sun,Pengfei Zhang,Linran Zeng,Krasimir Vasilev,Yunpeng Zhao,Yu Chen,Peilai Liu

Experimental & molecular medicine 55:587-596 PubMed36869070

2023

Circulating MiRNA-21-enriched extracellular vesicles promote bone remodeling in traumatic brain injury patients.

Applications

Unspecified application

Species

Unspecified reactive species

Ze Lin,Yuan Xiong,Yun Sun,Ruiyin Zeng,Hang Xue,Yiqiang Hu,Lang Chen,Guodong Liu,Adriana C Panayi,Wu Zhou,Faqi Cao,Fei Gao,Bobin Mi,Guohui Liu

International journal of molecular sciences 24: PubMed36769123

2023

BMSC-Derived Exosomal CircHIPK3 Promotes Osteogenic Differentiation of MC3T3-E1 Cells via Mitophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Shaoyang Ma,Sijia Li,Yuchen Zhang,Jiaming Nie,Jiao Cao,Ang Li,Ye Li,Dandan Pei

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Osteocalcin antibody [EPR3690]

Western blot - Anti-Osteocalcin antibody [EPR3690] (AB133612)

Western blot - Anti-Osteocalcin antibody [EPR3690] (AB133612)

Western blot - Anti-Osteocalcin antibody [EPR3690] (AB133612)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (71)

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