AB166709

Anti-Osteopontin antibody [7C5H12]

Name: Anti-Osteopontin antibody [7C5H12] (ab166709) | Abcam
Brand: Abcam Limited
SKU: AB166709
Price: 510 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(22 Publications)

Anti-Osteopontin antibody [7C5H12] (ab166709) is a mouse monoclonal antibody detecting Osteopontin in Western Blot, IHC-P. Suitable for Human.

- Over 10 publications

View Alternative Names

BNSP, OPN, PSEC0156, SPP1, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1

6 Images

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Western blot - Anti-Osteopontin antibody [7C5H12] (AB166709)

All lanes:

Western blot - Anti-Osteopontin antibody [7C5H12] (ab166709) at 1/500 dilution

All lanes:

recombinant Human Osteopontin protein

Predicted band size: 35 kDa

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [7C5H12] (AB166709)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [7C5H12] (AB166709)

Immunohistochemical analysis of paraffin embedded, DAB-stained Human endometrial cancer tissue, labeling Osteopontin using ab166709 at a 1/200 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [7C5H12] (AB166709)

Immunohistochemical analysis of paraffin embedded, DAB-stained Human prostate cancer tissue labeling Osteopontin using ab166709 at a 1/200 dilution.

IHC-P

AbReview41915****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [7C5H12] (AB166709)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for Osteopontin using ab166709 at 1/100 dilution in immunohistochemical analysis.

This image is courtesy of an Abreview submitted by Mrs. Maria Cecilia Ybanez.

IHC-P

AbReview41660****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [7C5H12] (AB166709)

ab166709 staining Osteopontin in human colon (smooth muscle cells) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in Tris pH 9.0. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An undiluted Biotin-conjugated horse anti-mouse IgG polyclonal was used as the secondary antibody.

This image is courtesy of an Abreview submitted by Steffen Rickelt

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Western blot - Anti-Osteopontin antibody [7C5H12] (AB166709)

All lanes:

Western blot - Anti-Osteopontin antibody [7C5H12] (ab166709) at 1/500 dilution

Lane 1:

HEK293 cell lysate

Lane 2:

HEK293 cell lysate, transfected with recombinant Human Osteopontin fragment (amino acids 167-314)

Predicted band size: 35 kDa,38 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7C5H12

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human SPP1 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P10451

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Osteopontin antibody [7C5H12] (ab166709) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Osteopontin?
Anti-Osteopontin [7C5H12] (ab166709) specifically detects a band for Osteopontin (UniProt: P10451) at a molecular weight of 35kDa.

Trusted by the scientific community
Anti-Osteopontin [7C5H12] (ab166709) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.

Biological function summary

Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.

Pathways

Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.

Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

See full target information SPP1

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Publications (22)

Recent publications for all applications. Explore the full list and refine your search

Materials today. Bio 33:102027 PubMed40677394

2025

Deliver CEBPE via cartilage targeting Lipid nanoparticle to block CEBPE-LTF-STAT3 positive feedback loop for efficient treatment of cartilage endplate degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangminghao Zhao,Peichuan Xu,Tao Li,Jinghong Yuan,Wenrui Zhao,Rui Ding,Guoyu Yang,Jiajun Xie,Guanfeng Huang,Zhidong Peng,Xinxin Miao,Xigao Cheng

Frontiers in bioengineering and biotechnology 13:1521134 PubMed39995594

2025

Gingival fibroblast suppress the osteogenesis process mediated by bone substitute materials via WNT/β-catenin signaling pathway and .

Applications

Unspecified application

Species

Unspecified reactive species

Guanqi Liu,Jiahui Lin,Xiaoyan Chen,Runheng Liu

Chinese medicine 20:3 PubMed39754271

2025

Magnolia kobus DC. suppresses neointimal hyperplasia by regulating ferroptosis and VSMC phenotypic switching in a carotid artery ligation mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Jong Min Kim,Yiseul Kim,Hyun-Jin Na,Haeng Jeon Hur,Sang Hee Lee,Mi Jeong Sung

Frontiers in physiology 14:1259567 PubMed37745241

2023

The accordion technique enhances bone regeneration via angiogenesis factor in a rat distraction osteogenesis model.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Liu,Sulong Wang,Ainizier Yalikun,Peng Ren,Aihemaitijiang Yusufu

Cell cycle (Georgetown, Tex.) 21:1932-1944 PubMed35574918

2022

LncRNA NEAT1 affects endometrial receptivity by regulating HOXA10 promoter activity.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxuan Geng,Chenchen Cui,Yisha Yin,Yan Zhao,Cuilian Zhang

Acta biochimica et biophysica Sinica 54:137-147 PubMed35130629

2022

SNHG1 functions as an oncogenic lncRNA and promotes osteosarcoma progression by up-regulating S100A6 via miR-493-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Liu,Jiaying Luo,Huan Wang,Lei Zhang,Guoxin Jin

Journal of tissue engineering and regenerative medicine 16:290-296 PubMed34965018

2022

Demineralized bone matrix combined with cytotoxic T-lymphocyte-associated protein 4 promotes osteogenic differentiation of human bone marrow mesenchymal stem cells and suppresses the activation of T lymphocytes in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Song,Rui Zhou,Jun Xiao,Lei He,Fang Zhu,Congcan Li,Fei Dai

Aging 13:23361-23375 PubMed34645714

2021

piRNA-36741 regulates BMP2-mediated osteoblast differentiation via METTL3 controlled m6A modification.

Applications

Unspecified application

Species

Unspecified reactive species

Jianmin Liu,Ming Chen,Longyang Ma,Xingbo Dang,Gongliang Du

JCI insight 6: PubMed34622803

2021

Therapeutic MK2 inhibition blocks pathological vascular smooth muscle cell phenotype switch.

Applications

Unspecified application

Species

Unspecified reactive species

J William Tierney,Brian C Evans,Joyce Cheung-Flynn,Bo Wang,Juan M Colazo,Monica E Polcz,Rebecca S Cook,Colleen M Brophy,Craig L Duvall

Frontiers in molecular biosciences 8:729789 PubMed34621787

2021

Development of a Toll-Like Receptor-Based Gene Signature That Can Predict Prognosis, Tumor Microenvironment, and Chemotherapy Response for Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lixia Liu,Bin Liu,Jie Yu,Dongyun Zhang,Jianhong Shi,Ping Liang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Osteopontin antibody [7C5H12]

Western blot - Anti-Osteopontin antibody [7C5H12] (AB166709)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [7C5H12] (AB166709)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [7C5H12] (AB166709)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [7C5H12] (AB166709)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [7C5H12] (AB166709)

Western blot - Anti-Osteopontin antibody [7C5H12] (AB166709)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (22)

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