AB11503

Anti-Osteopontin antibody

Name: Anti-Osteopontin antibody (ab11503) | Abcam
Brand: Abcam Limited
SKU: AB11503
Price: 420 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(21 Publications)

Goat Polyclonal Osteopontin antibody. Suitable for BL, WB, FuncS (Neut/Block), I-ELISA, ICC/IF, IHC-Fr and reacts with Mouse, Rat samples. Cited in 21 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Spp1.

View Alternative Names

Eta-1, Op, Spp-1, Spp1, Osteopontin, 2AR, Bone sialoprotein 1, Calcium oxalate crystal growth inhibitor protein, Early T-lymphocyte activation 1 protein, Minopontin, Secreted phosphoprotein 1, SPP-1

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-Fr, I-ELISA, ICC/IF, WB, FuncS (Neut/Block), BL

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Spp1.

P10923

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "BL" : {"fullname" : "Blocking", "shortname":"BL"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "BL-species-checked": "guaranteed", "BL-species-dilution-info": "", "BL-species-notes": "Blocks osteopontin in smooth muscle cell samples.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 μg/ml in the presence of 2 μg/ml of recombinant mouse osteopontin using 293 cells.", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "BL-species-checked": "guaranteed", "BL-species-dilution-info": "", "BL-species-notes": "Blocks osteopontin in smooth muscle cell samples.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FuncSNeutBlock-species-checked": "guaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "The Neutralization Dose50 (ND50) for anti-mouse osteopontin is approximately 1 to 3 μg/ml in the presence of 2 μg/ml of recombinant mouse osteopontin using 293 cells.", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Constituents: PBS, 5% Trehalose

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.

Biological function summary

Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.

Pathways

Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.

Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

See full target information Spp1

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Toxics 13: PubMed40423481

2025

Pulmonary Toxicity of Long, Thick MWCNT and Very Long, Thin Carboxylated MWCNT Aerosols Following 28 Days Whole-Body Exposure.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Guo,Matthew D Wright,Alison Buckley,Adam Laycock,Trine Berthing,Ulla Vogel,Frédéric Cosnier,Laurent Gaté,Martin O Leonard,Rachel Smith

Journal of tissue engineering 15:20417314241306681 PubMed39712077

2024

Harnessing bone marrow mesenchymal stem cell-derived extracellular vesicles and biomimetic peptide WKYMVm in self-healing hydrogel for enhanced bone repair in femoral defects.

Applications

Unspecified application

Species

Unspecified reactive species

Keyu Luo,Yufei Jin,Baiyi Liu,Yingbo Wang,Yaoyao Liu,Si Qiu,Jianhua Zhao,Xiang Yin

Frontiers in nephrology 4:1385705 PubMed39165274

2024

Short-term therapy with R568 ameliorated secondary hyperparathyroidism but does not prevent aortic valve calcification in uremic rats.

Applications

Unspecified application

Species

Unspecified reactive species

Asmahan Abu-Snieneh,Irina Gurt,Suzan Abedat,Chaim Lotan,Michael Glikson,Mony Shuvy

Cancer science 115:2565-2577 PubMed38932521

2024

FoxG1/BNIP3 axis promotes mitophagy and blunts cisplatin resistance in osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Baolong Pan,Yan Li,Huiyun Han,Lu Zhang,Xuemei Hu,Yanyu Pan,Zhuohui Peng

Journal of nanobiotechnology 21:459 PubMed38037135

2023

Biomimetic composite hydrogel promotes new bone formation in rat bone defects through regulation of miR-19b-3p/WWP1 axis by loaded extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Rongkang Guo,Chaohan Wu,Fan Liu,Tianhua Dong,Tao Zhang

Cells 12: PubMed36980197

2023

Mechanistic Insights into the Role of OPN in Mediating Brain Damage via Triggering Lysosomal Damage in Microglia/Macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Chengcheng Gai,Yijing Zhao,Danqing Xin,Tingting Li,Yahong Cheng,Zige Jiang,Yan Song,Dexiang Liu,Zhen Wang

Signal transduction and targeted therapy 8:41 PubMed36681678

2023

Application of metabolomics in urolithiasis: the discovery and usage of succinate.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu-Zhen Zhang,Xiong-Xin Lei,Yan-Lin Jiang,Long-Mei Zhao,Chen-Yu Zou,Yun-Jin Bai,Ya-Xing Li,Rui Wang,Qian-Jin Li,Qiu-Zhu Chen,Ming-Hui Fan,Yu-Ting Song,Wen-Qian Zhang,Yi Zhang,Jesse Li-Ling,Hui-Qi Xie

Histology and histopathology 38:1219-1229 PubMed36633331

2022

Hyperin up-regulates miR-7031-5P to promote osteogenic differentiation of MC3T3-E1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dongchen Qian,Yueyue Chen,Xusheng Qiu,Baohua Zhu,Lin Zhang,Yifeng Yan,Yixin Chen

American journal of translational research 14:7726-7743 PubMed36505285

2022

Osteopontin-integrin signaling positively regulates neuroplasticity through enhancing neural autophagy in the peri-infarct area after ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Haikang Liao,Zhenyou Zou,Weiqin Liu,Xuefeng Guo,Jinlu Xie,Liangxian Li,Xia Li,Xinying Gan,Xiansheng Huang,Juxia Liu,Wenyang Li,Hongji Zeng,Zheng Chen,Qiuhua Jiang,Hua Yao

Molecular medicine (Cambridge, Mass.) 28:121 PubMed36192693

2022

Induction of ferroptosis promotes vascular smooth muscle cell phenotypic switching and aggravates neointimal hyperplasia in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Shunchi Zhang,Yanrou Bei,Yueling Huang,Yimin Huang,Lianjie Hou,Xi-Long Zheng,Yiming Xu,Shaoguo Wu,Xiaoyan Dai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Osteopontin antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

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