AB216402

Anti-Osteopontin antibody

Name: Anti-Osteopontin antibody (ab216402) | Abcam
Brand: Abcam Limited
SKU: AB216402
Price: 525 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal Osteopontin antibody. Suitable for Flow Cyt, IHC-P and reacts with Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Mouse Spp1 aa 200 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

BNSP, OPN, PSEC0156, SPP1, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1

4 Images

Flow Cytometry - Anti-Osteopontin antibody (AB216402)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Osteopontin antibody (AB216402)

HepG2 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 0.1%PBST for 20 min at room temperature, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with Osteopontin Polyclonal Antibody (ab216402) at 1 : 100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB216402)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB216402)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat shin bone tissue tissue labeling Osteopontin with ab216402 at 1/300 dilution followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB216402)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat hippocampus tissue tissue labeling Osteopontin with ab216402 at 1/300 dilution followed by conjugation to the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB216402)

Paraformaldehyde-fixed, paraffin embedded Rat lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with Osteopontin Polyclonal Antibody, Unconjugated (ab216402) at 1 : 400 overnight at 4°C, DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Mouse Spp1 aa 200 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P10923

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.

Biological function summary

Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.

Pathways

Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.

Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.

See full target information SPP1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 46:406-422 PubMed39850022

2025

Injectable thermosensitive antibiotic-laden chitosan hydrogel for regenerative endodontics.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandre Henrique Dos Reis-Prado,Maedeh Rahimnejad,Renan Dal-Fabbro,Priscila Toninatto Alves de Toledo,Caroline Anselmi,Pedro Henrique Chaves de Oliveira,J Christopher Fenno,Luciano Tavares Angelo Cintra,Francine Benetti,Marco C Bottino

ACS applied materials & interfaces 15:32121-32135 PubMed37364054

2023

Nanoscale β-TCP-Laden GelMA/PCL Composite Membrane for Guided Bone Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Abdel H Mahmoud,Yuanyuan Han,Renan Dal-Fabbro,Arwa Daghrery,Jinping Xu,Darnell Kaigler,Sarit B Bhaduri,Jos Malda,Marco C Bottino

TheScientificWorldJournal 2022:6740853 PubMed36561943

2022

The Cytokine and Bone Protein Expression by Ellagic Acid-Hydroxyapatite in Bone Remodelling Model.

Applications

Unspecified application

Species

Unspecified reactive species

Dyah Noviana Primasari,Intan Nirwana,Hendrik Setia Budi,Agung Satria Wardhana,Annisa Fitria Sari,Novita Novita,Andika Putri Setyawan,Meircurius Dwi Condro Surboyo,Khairul Anuar Shariff

Advanced healthcare materials 12:e2201886 PubMed36496552

2022

Freeze-Casted Keratin Matrix as an Organic Binder to Integrate Hydroxyapatite and BMP2 for Enhanced Cranial Bone Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Wan Ting Sow,Yili Wang,Liping Chen,Qiling Lai,Qiong Chen,Linjie Chen,Chunwu Zhang,Huaqiong Li

Frontiers in oncology 12:877147 PubMed35707368

2022

Evaluation of Galanin Expression in Colorectal Cancer: An Immunohistochemical and Transcriptomic Study.

Applications

Unspecified application

Species

Unspecified reactive species

Iman M Talaat,Nada M Yakout,Ahmed S A Soliman,Thenmozhi Venkatachalam,Arya Vinod,Leen Eldohaji,Vidhya Nair,Amal Hareedy,Alaa Kandil,Wael M Abdel-Rahman,Rifat Hamoudi,Maha Saber-Ayad

Journal of cellular biochemistry 120:19422-19431 PubMed31452264

2019

Overexpressed miR-196a accelerates osteogenic differentiation in osteoporotic mice via GNAS-dependent Hedgehog signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Na Zhong,Yu-Zhu Zhang,Hong Li,Hui-Ling Fu,Cheng-Xiu Lv,Xiu-Juan Jia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Osteopontin antibody

Flow Cytometry - Anti-Osteopontin antibody (AB216402)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB216402)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB216402)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB216402)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise