Anti-Osteopontin antibody ab63856 is a rabbit polyclonal antibody that is used in Osteopontin western blotting, IHC and immunofluorescence. Suitable for human samples.
- Tried and trusted by researchers since 2009
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- Clinical and translational medicine 13:e15152023Single-cell and spatial transcriptomics reveal POSTN cancer-associated fibroblasts correlated with immune suppression and tumour progression in non-small cell lung cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Chen,Qiang Guo,Yang Liu,Qinghua Hou,Mengying Liao,Yanying Guo,Yupeng Zang,Fei Wang,Huanyu Liu,Xinyu Luan,Yanling Liang,Zhuojue Guan,Yanling Li,Haozhen Liu,Xuan Dong,Xiuqing Zhang,Jixian Liu,Qumiao XuPubMed 38115703
- BMC oral health 23:5142023Can locally applied risedronate be an effective agent when combined with xenografts? An animal study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTaha Özer,Vusala Guliyeva,Alper Aktaş,Emre Barış,Mert OcakPubMed 37488504
- Nature communications 14:40912023In situ activation of flexible magnetoelectric membrane enhances bone defect repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenwen Liu,Han Zhao,Chenguang Zhang,Shiqi Xu,Fengyi Zhang,Ling Wei,Fangyu Zhu,Ying Chen,Yumin Chen,Ying Huang,Mingming Xu,Ying He,Boon Chin Heng,Jinxing Zhang,Yang Shen,Xuehui Zhang,Houbing Huang,Lili Chen,Xuliang DengPubMed 37429900
- Orthopaedic surgery 15:2423-24342023ASPN Synergizes with HAPLN1 to Inhibit the Osteogenic Differentiation of Bone Marrow Mesenchymal Stromal Cells and Extracellular Matrix Mineralization of Osteoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuohui Zhou,Xinmin Yan,Zhenfei Chen,Xing Zeng,Fangqian WuPubMed 37427673
- International journal of molecular sciences 24:2023Low Magnesium Concentration Enforces Bone Calcium Deposition Irrespective of 1,25-Dihydroxyvitamin D Concentration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUsman Rashid,Sandra K Becker,Gerhard Sponder,Susanne Trappe,Mansur A Sandhu,Jörg R AschenbachPubMed 37240030
- Cellular signalling 108:1106942023Advanced glycation end products inhibit the osteogenic differentiation potential of adipose-derived stem cells in mice through autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Fu,Qiang Zhu,Fangzhi Lou,Shuyu Cai,Shuanglin Peng,Jingang XiaoPubMed 37141927
- Journal of orthopaedic surgery and research 18:3122023Network pharmacology identifies fisetin as a treatment for osteoporosis that activates the Wnt/β-catenin signaling pathway in BMSCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuihong Liang,Jinlong Zhao,Jianke Pan,Yuan Yang,Yaoxing Dou,Weiyi Yang,Lingfeng Zeng,Jun LiuPubMed 37087476
- Clinical and translational medicine 13:e12372023Immune landscape and the key role of APOE+ monocytes of lupus nephritis under the single-cell and spatial transcriptional vista.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYouzhou Tang,Ying Zhang,Xinyu Li,Ruoyao Xu,Ying Ji,Jishi Liu,Jianye Liu,Quan Zhuang,Hao ZhangPubMed 37026377
- Biomaterials translational 4:27-402023Mechanically conditioned cell sheets cultured on thermo-responsive surfaces promote bone regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGen Wang,Zhangqin Yuan,Li Yu,Yingkang Yu,Pinghui Zhou,Genglei Chu,Huan Wang,Qianping Guo,Caihong Zhu,Fengxuan Han,Song Chen,Bin LiPubMed 37206307
- Biomedical engineering online 22:152023Hydroxyapatite microbeads containing BMP-2 and quercetin fabricated via electrostatic spraying to encourage bone regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeoyun Lee,Honghyun Park,Jeong-Seop Oh,Kyubin Byun,Dae-Yong Kim,Hui-Suk Yun,Byung-Jae KangPubMed 36803418
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Expected | Tested | Expected |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Associated Products
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Target data
Function
Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Alternative names
BNSP, OPN, PSEC0156, SPP1, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1
Recommended products
Anti-Osteopontin antibody ab63856 is a rabbit polyclonal antibody that is used in Osteopontin western blotting, IHC and immunofluorescence. Suitable for human samples.
- Tried and trusted by researchers since 2009
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.
- Biological function summary
Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.
- Pathways
Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.
- Associated diseases and disorders
Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (ab63856)
IHC image of Osteopontin staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab63856, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody (ab63856)
ab63856 staining Osteopontin in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab63856 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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