Anti-Osteopontin antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB8448)

Human Breast tumour section. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section). There is also weak staining of the extracellular matrix. Osteopontin is not believed to be expressed inside breast tumour cells, and there is no staining in the intracellular region of the breast cells in this section.

Osteopontin antibody (ab8448) used at 1 : 100-1 : 300. No antigen retrieval is required.

• Schematic Diagram

PubMed

Schematic Diagram - Anti-Osteopontin antibody (AB8448)

OPN is cleaved by MMP to yield 2 fragments, which migrate at 40kD(N terminal) and 32kD (C terminal). The C terminal fragment can undergo further cleavage by both of these MMPs (see Agnihotri et al, JBC 2001 for further details). The epitope recognised by ab8448 is shown in violet. This antibody detects the full length OPN and the 32kD fragment. It does not recognise the 40kD fragment.

This image is courtesy of Agnihotri et al, JBC 2001

• IHC-P

CiteAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB8448)

Osteopontin Immunohistochemistry using Anti-Osteopontin antibody ab8448. Publication image and figure legend from Lin, C. N., Wang, C. J., et al., 2015, BMC Cancer, PubMed 25872762.

The existence of OPN and M2-TAMs in gastric cancer specimens; the M2-TAMs were stained with a CD204-specific antibody. (a) The stain intensity is shown from grade 0 to grade C. (b, c) The expression of OPN and CD204 was significantly correlated with the tumor stage (p < 0.05). (d) The OPN score was positively correlated with the CD204 score (p = 0.0078).

• IHC-P

CiteAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (AB8448)

Immunohistochemistry using Anti-Osteopontin antibody, ab8448. Publication image and figure legend from Gui, X., Qiu, X., et al.,2020, Biomed Res Int, PubMed 32104688.

ab8448 was used in this publication in immunohistochemistry. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab8448 please see the product overview.

(a, b) No obvious staining for OPN was observed in the control lung. (original magnification : ×200/400). (c, d) In explanted lungs from IPF patients, the expression of OPN was found in alveolar epithelial cells lining honeycomb space and alveolar macrophages accumulating in alveolar space adjacent to fibrotic lesions (original magnification : ×200/400).