Anti-Osteopontin antibody is a rabbit polyclonal antibody that is used in SPP1 in IHC. Suitable for human samples. It recognizes the full-length osteopontin protein (66 kDa) and C-terminal fragments of both thrombin and MMP-cleaved OPN (32 kDa). It does not recognise the N-terminal fragment (40 kDa).
- Cited in over 440 publications
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- Pharmacology 109:34-422023Impairment of Autophagy Mediates the Uric-Acid-Induced Phenotypic Transformation of Vascular Smooth Muscle Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Lu,Hanlin Zhang,Min Han,Ping Wang,Liping MengPubMed 38011839
- Materials (Basel, Switzerland) 16:2023Development of 3D Printable Calcium Phosphate Cement Scaffolds with Cockle Shell Powders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEunbee Cho,Jae Eun Kim,Juo Lee,Sangbae Park,Sungmin Lee,Jong Hoon Chung,Jungsil Kim,Hoon SeonwooPubMed 37763431
- International journal of molecular sciences 24:2023Novel Electroactive Mineralized Polyacrylonitrile/PEDOT:PSS Electrospun Nanofibers for Bone Repair Applications.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrederico Barbosa,Fábio F F Garrudo,Ana C Marques,Joaquim M S Cabral,Jorge Morgado,Frederico Castelo Ferreira,João C SilvaPubMed 37686010
- Science and technology of advanced materials 24:22422422023Hydroxyapatite-filled osteoinductive and piezoelectric nanofibers for bone tissue engineering.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrederico Barbosa,Fábio F F Garrudo,Paola S Alberte,Leonor Resina,Marta S Carvalho,Akhil Jain,Ana C Marques,Francesc Estrany,Frankie J Rawson,Carlos Aléman,Frederico Castelo Ferreira,João C SilvaPubMed 37638280
- Journal of functional biomaterials 14:2023Fabrication and Evaluation of Porous dECM/PCL Scaffolds for Bone Tissue Engineering.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiwei Wang,Xiaqing Zhou,Zhuozhuo Yin,Xiaojun YuPubMed 37504838
- Small methods 7:e23003702023Seamlessly Adhesive Bionic Periosteum Patches Via Filling Microcracks for Defective Bone Healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu Yang,Zhijie Chen,Pengzhen Zhuang,Yunkai Tang,Zehao Chen,Fei Wang,Zhengwei Cai,Jie Wei,Wenguo CuiPubMed 37356079
- International journal of molecular sciences 24:2023Influence of Occlusal Hypofunction on Alveolar Bone Healing in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Damanaki,Svenja Beisel-Memmert,Marjan Nokhbehsaim,Ali Abedi,Birgit Rath-Deschner,Andressa V B Nogueira,James DeschnerPubMed 37298695
- Frontiers in immunology 14:11062892023Deciphering the single-cell transcriptome network in keloids with intra-lesional injection of triamcinolone acetonide combined with 5-fluorouracil.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYijun Xia,Youbin Wang,Yan Hao,Mengjie Shan,Hao Liu,Zhengyun Liang,Xinwen KuangPubMed 37275903
- Stem cell research & therapy 14:1302023Epithelium-derived SCUBE3 promotes polarized odontoblastic differentiation of dental mesenchymal stem cells and pulp regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZijie Wang,Chuying Chen,Jiayi Zhang,Jiangdie He,Lin Zhang,Jiayuan Wu,Zhihui TianPubMed 37189178
- APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 131:313-3242023Circ_0006873 suppresses the osteogenic differentiation of human-derived mesenchymal stem cells through mediating miR-20a/SMURF2 axis in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiangna Pang,Yongfu Wu,Yanlin Ji,Yilan Si,Fang LiangPubMed 37185991
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 98.6% Whole serum, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SPP1 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P10451
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Tested | Not recommended |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes at this dilution, the antibody will strongly detect approximately 250 ng of OPN protein on a blot. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/300 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
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Target data
Function
Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Alternative names
BNSP, OPN, PSEC0156, SPP1, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1
Recommended products
Anti-Osteopontin antibody is a rabbit polyclonal antibody that is used in SPP1 in IHC. Suitable for human samples. It recognizes the full-length osteopontin protein (66 kDa) and C-terminal fragments of both thrombin and MMP-cleaved OPN (32 kDa). It does not recognise the N-terminal fragment (40 kDa).
- Cited in over 440 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 98.6% Whole serum, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SPP1 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link P10451
- Purity
- Whole antiserum
- Specificity
Osteopontin is directed against human osteopontin. The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Osteopontin antibody (ab8448) is a rabbit polyclonal antibody and is validated for use in IHC-P in human samples.
Anti-Osteopontin antibody (ab8448) specifically detects Osteopontin (UniProt ID: P10451; Molecular weight: 34kDa) and is sold in 200 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Osteopontin antibody (ab8448) has high sensitivity and specificity.
Anti-Osteopontin antibody (ab8448) has been cited over 445 times in peer reviewed journals and is trusted by the scientific community.
Anti-Osteopontin antibody (ab8448) has 14 independent reviews from customers.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (ab8448)
Human Breast tumour section. Osteopontin is a normal component of elastic fibers in the breast (shown heavily stained in this section). There is also weak staining of the extracellular matrix. Osteopontin is not believed to be expressed inside breast tumour cells, and there is no staining in the intracellular region of the breast cells in this section.
Osteopontin antibody (ab8448) used at 1:100-1:300. No antigen retrieval is required.
This image is courtesy of Agnihotri et al, JBC 2001Schematic Diagram - Anti-Osteopontin antibody (ab8448)
OPN is cleaved by MMP to yield 2 fragments, which migrate at 40kD(N terminal) and 32kD (C terminal). The C terminal fragment can undergo further cleavage by both of these MMPs (see Agnihotri et al, JBC 2001 for further details). The epitope recognised by ab8448 is shown in violet. This antibody detects the full length OPN and the 32kD fragment. It does not recognise the 40kD fragment.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody (ab8448)
Osteopontin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining using rabbit Anti-Osteopontin antibody
Immunohistochemistry using Anti-Osteopontin antibody, ab8448. Publication image and figure legend from Gui, X., Qiu, X., et al.,2020, Biomed Res Int, PubMed 32104688.
ab8448 was used in this publication in immunohistochemistry. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab8448 please see the product overview.
(a, b) No obvious staining for OPN was observed in the control lung. (original magnification: ×200/400). (c, d) In explanted lungs from IPF patients, the expression of OPN was found in alveolar epithelial cells lining honeycomb space and alveolar macrophages accumulating in alveolar space adjacent to fibrotic lesions (original magnification: ×200/400).
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