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Rabbit Polyclonal Osteopontin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 445 publications. Immunogen corresponding to Synthetic Peptide within Human SPP1 aa 150-200 conjugated to Keyhole Limpet Haemocyanin.
Alternative names=Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1, PSEC0156, OPN, BNSP, SPP1
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
Synthetic Peptide within Human SPP1 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link: P10451
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Not recommended | Tested | Not recommended |
Images
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes at this dilution, the antibody will strongly detect approximately 250 ng of OPN protein on a blot. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/300 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Recommended products
Rabbit Polyclonal Osteopontin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 445 publications. Immunogen corresponding to Synthetic Peptide within Human SPP1 aa 150-200 conjugated to Keyhole Limpet Haemocyanin.
Alternative names=Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1, PSEC0156, OPN, BNSP, SPP1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Preservative: 0.1% Sodium azide
Constituents: 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
Synthetic Peptide within Human SPP1 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link: P10451
- Purity
Whole antiserum
- Specificity
Osteopontin is directed against human osteopontin. The antibody recognizes the full-length osteopontin protein (which runs at 66 kDa on westerns), as well as the C-terminal fragments of both thrombin and MMP-cleaved OPN. The 32 kDa MMP-cleaved C-fragment is recognized, but not the 40 kDa N-terminal fragment. Reactivity is reported to occur with osteopontin from swine, dog, mouse and rat.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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