Rabbit Recombinant Monoclonal Osteopontin antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse samples. Cited in 11 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (AB218237), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21138-ab218237--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126179.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (AB218237)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (AB218237), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21138-ab218237--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133897.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (AB218237)")
![Western blot - Anti-Osteopontin antibody [EPR21138] (AB218237), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21138-ab218237--western-blot-img417484.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Osteopontin antibody [EPR21138] (AB218237)")
![Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21138] (AB218237), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21138-ab218237--immunocytochemistry-immunofluorescence-img400229.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21138] (AB218237)")
Publications
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- Journal of neuroinflammation 20:2942023Lateralized response of skull bone marrow via osteopontin signaling in mice after ischemia reperfusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChaoran Xu,Qia Zhang,Yi Zhang,Huaijun Chen,Tianchi Tang,Junjie Wang,Siqi Xia,Gao Chen,Jianmin ZhangPubMed 38071333
- Cells 12:2023Influence of Extracellular Matrix Components on the Differentiation of Periodontal Ligament Stem Cells in Collagen I Hydrogel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexey A Ivanov,Alla V Kuznetsova,Olga P Popova,Tamara I Danilova,Andrey V Latyshev,Oleg O YanushevichPubMed 37830549
- Journal of periodontal research 58:414-4212023Loss of β-catenin causes cementum hypoplasia by hampering cementogenic differentiation of Axin2-expressing cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Ma,Xudong Xie,Chunmei Xu,Peilei Shi,Yafei Wu,Jun WangPubMed 36691857
- Biomolecules 13:2023Decellularized Matrix Induced Spontaneous Odontogenic and Osteogenic Differentiation in Periodontal Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexey A Ivanov,Tamara I Danilova,Alla V Kuznetsova,Olga P Popova,Oleg O YanushevichPubMed 36671506
- Cell stem cell 29:1213-1228.e82022Dynamic and adaptive cancer stem cell population admixture in colorectal neoplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEster Gil Vasquez,Nadia Nasreddin,Gabriel N Valbuena,Eoghan J Mulholland,Hayley L Belnoue-Davis,Holly R Eggington,Ryan O Schenck,Valérie M Wouters,Pratyaksha Wirapati,Kathryn Gilroy,Tamsin R M Lannagan,Dustin J Flanagan,Arafath K Najumudeen,Sulochana Omwenga,Amy M B McCorry,Alistair Easton,Viktor H Koelzer,James E East,Dion Morton,Livio Trusolino,Timothy Maughan,Andrew D Campbell,Maurice B Loughrey,Philip D Dunne,Petros Tsantoulis,David J Huels,Sabine Tejpar,Owen J Sansom,Simon J LeedhamPubMed 35931031
- Cell metabolism 34:1064-1078.e62022Mapping the single-cell transcriptomic response of murine diabetic kidney disease to therapies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaojia Wu,Romer Gonzalez Villalobos,Xiang Yao,Dermot Reilly,Tao Chen,Matthew Rankin,Eugene Myshkin,Matthew D Breyer,Benjamin D HumphreysPubMed 35709763
- Journal of anatomy 240:385-3972021Osteogenic and angiogenic profiles of the palatal process of the maxilla and the palatal process of the palatine bone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCaiyu Liao,Meng Lu,Yuhang Hong,Chuanqing Mao,Jiangping Chen,Chengyan Ren,Minkui Lin,Weihui ChenPubMed 34569061
- Translational oncology 14:1012282021Transgenic overexpression of the miR-200b/200a/429 cluster inhibits mammary tumor initiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatrina L Watson,Rui Yi,Roger A MooreheadPubMed 34562686
- Cells 10:2021Single-Cell RNA-Seq Reveals Transcriptomic Heterogeneity and Post-Traumatic Osteoarthritis-Associated Early Molecular Changes in Mouse Articular Chondrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAimy Sebastian,Jillian L McCool,Nicholas R Hum,Deepa K Murugesh,Stephen P Wilson,Blaine A Christiansen,Gabriela G LootsPubMed 34200880
- Scientific reports 10:171502020Essential roles of oncostatin M receptor β signaling in renal crystal formation in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShimpei Yamashita et. al.PubMed 33051515
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Mouse | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Alternative names
Eta-1, Op, Spp-1, Spp1, Osteopontin, 2AR, Bone sialoprotein 1, Calcium oxalate crystal growth inhibitor protein, Early T-lymphocyte activation 1 protein, Minopontin, Secreted phosphoprotein 1, SPP-1
Recommended products
Rabbit Recombinant Monoclonal Osteopontin antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse samples. Cited in 11 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR21138
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is not recommended for use in WB with mouse tissue samples.
Osteopontin is a secretory protein, to allow the detection of Osteopontin in cell lysate, we suggest treating cells with BFA which inhibits protein secretion.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.
- Biological function summary
Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.
- Pathways
Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.
- Associated diseases and disorders
Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (ab218237), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21138-ab218237--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126179.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (ab218237)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (ab218237)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Osteopontin with ab218237 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in distal tubules of mouse kidney (PMID: 17938278). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (ab218237), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21138-ab218237--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133897.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (ab218237)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21138] (ab218237)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling Osteopontin with ab218237 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in biliary epithelial cells of mouse liver (PMID: 16221502). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Osteopontin antibody [EPR21138] (ab218237), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21138-ab218237--western-blot-img417484.jpg "Western blot - Anti-Osteopontin antibody [EPR21138] (ab218237)")
Western blot - Anti-Osteopontin antibody [EPR21138] (ab218237)
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a loading control.
This antibody is not recommended for use in WB with mouse tissue samples.
We suggest treating cells with BFA which inhibits protein secretion.All lanes: Western blot - Anti-Osteopontin antibody [EPR21138] (ab218237) at 1/1000 dilution
Lane 1: Mouse kidney tissue lysate at 20 µg
Lane 2: Mouse placenta tissue lysate at 20 µg
Lane 3: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 4: RAW 264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 4h whole cell lysate at 20 µg
Lane 5: RAW 264.7 treated with 1000 ng/ml BFA for 3h whole cell lysate at 20 µg
Lane 6: RAW 264.7 treated with 100 ng/ml lipopolysaccharide (LPS) for 4h then add 1000 ng/ml BFA for another 3h whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 60 kDa
Exposure time: 180s
![Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21138] (ab218237), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21138-ab218237--immunocytochemistry-immunofluorescence-img400229.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21138] (ab218237)")
Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21138] (ab218237)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labelling Osteopontin with primary antibody anti-Osteopontin (ab218237) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody at 1/1000 dilution. Confocal image showing increased cytoplasmic staining in RAW 264.7 cells treated with LPS (100 ng/ml) for 6 h, then add BFA (300 ng/ml) for 3 h. Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used to counterstain tubulin at 1/200 dilution. The nuclear counter stain is DAPI (blue).
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