Rabbit Recombinant Monoclonal Osteopontin antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Recombinant fragment - Human samples. Cited in 40 publications.
Images
![Immunoprecipitation - Anti-Osteopontin antibody [EPR21139-316] (AB214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--immunoprecipitation-img8576.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Osteopontin antibody [EPR21139-316] (AB214050)")
![Western blot - Anti-Osteopontin antibody [EPR21139-316] (AB214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--western-blot-img32579.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Osteopontin antibody [EPR21139-316] (AB214050)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] (AB214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65049.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] (AB214050)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] (AB214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img50011.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] (AB214050)")
![Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21139-316] (AB214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--immunocytochemistry-immunofluorescence-img181521.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21139-316] (AB214050)")
Publications
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- Cell communication and signaling : CCS 21:2922023Single-cell RNA sequencing reveals pro-invasive cancer-associated fibroblasts in hypopharyngeal squamous cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhimou Cai,Lin Chen,Siyu Chen,Ruihua Fang,Xiaolin Chen,Wenbin LeiPubMed 37853464
- iScience 26:1079232023Circ_0000888 regulates osteogenic differentiation of periosteal mesenchymal stem cells in congenital pseudarthrosis of the tibia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhuoyang Li,Yaoxi Liu,Yiyong Huang,Qian Tan,Haibo Mei,Guanghui Zhu,Kun Liu,Ge YangPubMed 37810257
- Genes & diseases 11:964-9772023deletion in chondrocytes causes vertebral defects by reducing MEF2C/PTEN/AKT signaling.Applications:WBReactive species:MouseChengzhi Wu,Hengyu Liu,Dongmei Zhong,Xiaoming Yang,Zhiheng Liao,Yuyu Chen,Shun Zhang,Deying Su,Baolin Zhang,Chuan Li,Liru Tian,Caixia Xu,Peiqiang SuPubMed 37692479
- International journal of molecular sciences 24:2023CXCR4 Expressed by Tumor-Infiltrating B Cells in Gastric Cancer Related to Survival in the Tumor Microenvironment: An Analysis Combining Single-Cell RNA Sequencing with Bulk RNA Sequencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChen Su,Rong Yu,Xiaoquan Hong,Panpan Zhang,Yingying Guo,Jian-Chun Cai,Jingjing HouPubMed 37629071
- Cell discovery 9:602023Multi-dimensional single-cell characterization revealed suppressive immune microenvironment in AFP-positive hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuisi He,Shuzhen Chen,Zhecai Fan,Yaping Dong,Ying Wang,Shiyao Li,Xiaojuan Sun,Yuting Song,Jinxian Yang,Qiqi Cao,Jie Jiang,Xianming Wang,Wen Wen,Hongyang WangPubMed 37336873
- Frontiers in cardiovascular medicine 10:11752872023Coronary plaque burden predicts perioperative cardiovascular events after coronary endarterectomy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingxin Gao,Wanwan Wen,Chengxiong Gu,XiaoLi Zhang,Yang Yu,Haiyang LiPubMed 37363096
- Pharmacogenomics and personalized medicine 16:425-4322023A Preliminary Study on the Correlation Between Age and Endometrial Receptivity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSong Guo,Di Zhang,Shan Zhao,Huan Zhang,Yijuan Sun,Li YanPubMed 37180957
- Advanced functional materials 33:2023Simulation of Cortical and Cancellous Bone to Accelerate Tissue Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhihai Fan,Hongxiang Liu,Zhaozhao Ding,Liying Xiao,Qiang Lu,David L KaplanPubMed 37601745
- Cellular oncology (Dordrecht) 46:1351-13682023Dissecting the single-cell transcriptome network of macrophage and identifies a signature to predict prognosis in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhengyang Hu,Xing Jin,Weifeng Hong,Qihai Sui,Mengnan Zhao,Yiwei Huang,Ming Li,Qun Wang,Cheng Zhan,Zhencong ChenPubMed 37079186
- Materials today. Bio 19:1005902023A novel implant surface modification mode of Fe3O4-containing TiO2 nanorods with sinusoidal electromagnetic field for osteoblastogenesis and angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRanyue Ren,Jiachao Guo,Hao Song,Yong Wei,Chao Luo,Yayun Zhang,Liangxi Chen,Biao Gao,Jijiang Fu,Wei XiongPubMed 36910272
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Recombinant fragment - Human | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
8 products for Alternative Product
1 products for Alternative Version
Target data
Function
Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Alternative names
Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1, PSEC0156, OPN, BNSP, SPP1
Recommended products
Rabbit Recombinant Monoclonal Osteopontin antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Recombinant fragment - Human samples. Cited in 40 publications.
Alternative names
Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1, PSEC0156, OPN, BNSP, SPP1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR21139-316
- Purification technique
Affinity purification Protein A
- Specificity
Osteopontin is a secretory protein, to allow the detection of Osteopontin in cell lysate, we suggest treating cells with BFA which inhibits protein secretion.
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-Osteopontin antibody [EPR21139-316] (ab214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--immunoprecipitation-img8576.jpg "Immunoprecipitation - Anti-Osteopontin antibody [EPR21139-316] (ab214050)")
Immunoprecipitation - Anti-Osteopontin antibody [EPR21139-316] (ab214050)
Osteopontin was immunoprecipitated from 0.35 mg of human kidney lysate with ab214050 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214050 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5,000 dilution
Lane 1: Human kidney lysate 10 μg (Input).
Lane 2: ab214050 IP in human kidney lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab214050 in human kidney lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
The multiple bands observed are likely due to expression of different isoforms, as well as glycosylation (PMID: 8761944; PMID: 11375993; PMID: 8535240; PMID: 12890718).
All lanes: Immunoprecipitation - Anti-Osteopontin antibody [EPR21139-316] (ab214050)
Predicted band size: 35 kDa
![Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--western-blot-img32579.jpg "Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050)")
Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050)
Blocking/Dilution buffer: 5% NFDM/TBST.
The his-tagged recombinant protein was produced in a mammalian expression system, thereby allowing it to be post-translationally modified.
All lanes: Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050) at 1/1000 dilution
All lanes: Human Osteopontin recombinant protein (aa17-300) produced in a mammalian expression at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 60-65 kDa
Exposure time: 26s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] (ab214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65049.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] (ab214050)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] (ab214050)
Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Osteopontin with ab214050 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in bile ducts of human liver (PMID: 16221502). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] (ab214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img50011.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] (ab214050)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [EPR21139-316] (ab214050)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Osteopontin with ab214050 at 1/2000 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) Ready to use. Cytoplasmic staining in distal tubules of human kidney (PMID: 17938278), performed on a Leica Biosystems BOND® RX instrument. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat-mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 minutes.
![Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21139-316] (ab214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--immunocytochemistry-immunofluorescence-img181521.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21139-316] (ab214050)")
Immunocytochemistry/ Immunofluorescence - Anti-Osteopontin antibody [EPR21139-316] (ab214050)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG cell line labelling Osteopontin with ab214050 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplamic staining in U-87 MG cell line. The nuclear counter stain is DAPI (blue).
The negative controls is as follows:
-ve control: Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.![Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--western-blot-img50087.jpg "Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050)")
Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050)
Blocking/Dilution buffer: 5% NFDM/TBST.
The multiple bands observed are likely due to expression of different isoforms, as well as glycosylation (PMID: 8761944; PMID: 11375993; PMID: 8535240; PMID: 12890718).
The identity of the 90kDa band is unknown.
All lanes: Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050) at 1/1000 dilution
Lane 1: Human urine at 20 µg
Lane 2: Human fetal kidney lysate at 10 µg
SecondaryLane 1: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Lane 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 45 kDa, 60 kDa, 70 kDa
Exposure time: 26s
![Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--western-blot-img428906.jpg "Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050)")
Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050)
Blocking and diluting buffer concentration: 5% NFDM/TBST.
We suggest treating cells with BFA which inhibits protein secretion.
All lanes: Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050) at 1/1000 dilution
Lane 1: Untreated HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: HepG2 treated with 300ng/ml BFA for 3h whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 60 kDa
Exposure time: 60s
![Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--western-blot-img50087.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050)")
![Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050), expandable thumbnail](https://content.abcam.com/products/images/osteopontin-antibody-epr21139-316-ab214050--western-blot-img428906.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Osteopontin antibody [EPR21139-316] (ab214050)")
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