Anti-Osteopontin antibody [RM1018]
- BOND RX™ Validated
- 20ul selling size
- RabMAb
- Recombinant
- What is this?
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(26 Publications)
Anti-Osteopontin antibody [RM1018] (ab283656) is a rabbit recombinant multiclonal antibody detecting Osteopontin in Western Blot, IP, IHC-P, ELISA. Suitable for Human, Mouse,.
- Biophysical QC for unrivalled batch-batch consistency
View Alternative Names
BNSP, OPN, PSEC0156, SPP1, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [RM1018] (AB283656)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling Osteopontin with ab283656 at 1/2000 (0.232 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the bile ducts in human liver. The section was incubated with ab283656 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [RM1018] (AB283656)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Osteopontin with ab283656 at 1/2000 (0.232 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on distal tubules in human kidney. The section was incubated with ab283656 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
- IP
Supplier Data
Immunoprecipitation - Anti-Osteopontin antibody [RM1018] (AB283656)
Osteopontin was immunoprecipitated from 0.35 mg Human kidney lysate 10ug with ab283656 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283656 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 : Human kidney lysate 10ug
Lane 2 : ab283656 IP in Human kidney lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab283656 in Human kidney lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 1 min.
All lanes:
Immunoprecipitation - Anti-Osteopontin antibody [RM1018] (ab283656)
Predicted band size: 35 kDa
Observed band size: 60 kDa
false
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteopontin antibody [RM1018] (AB283656)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling Osteopontin with ab283656 at 1/2000 (0.232 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on the bile ducts in mouse liver. The section was incubated with ab283656 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
- IP
Supplier Data
Immunoprecipitation - Anti-Osteopontin antibody [RM1018] (AB283656)
Osteopontin was immunoprecipitated from 0.35 mg RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml LPS for 3h then add 300ng/ml BFA for another 3h whole cell lysate 10ug with ab283656 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283656 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml LPS for 3h then add 300ng/ml BFA for another 3h whole cell lysate 10ug
Lane 2 : ab283656 IP in RAW 264.7 treated with 100ng/ml LPS for 3h then add 300ng/ml BFA for another 3h whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab283656 in RAW 264.7 treated with 100ng/ml LPS for 3h then add 300ng/ml BFA for another 3h whole cell lysate
Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 32 seconds.
Lower bands should be MMP-cleaved Osteopontin. (PMID : 11375993)
All lanes:
Immunoprecipitation - Anti-Osteopontin antibody [RM1018] (ab283656)
Predicted band size: 35 kDa
Observed band size: 60 kDa
false
- WB
Lab
Western blot - Anti-Osteopontin antibody [RM1018] (AB283656)
Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab181602 was used as a GAPDH loading control. This antibody is not recommended for use in WB with mouse tissue samples. We suggest treating cells with BFA which inhibits protein secretion.
All lanes:
Western blot - Anti-Osteopontin antibody [RM1018] (ab283656) at 1/1000 dilution
Lane 1:
Mouse kidney tissue lysate at 20 µg
Lane 2:
Mouse placenta tissue lysate at 20 µg
Lane 3:
RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 4:
RAW 264.7 treated with 100ng/ml lipopolysaccharide (LPS) for 4h whole cell lysate at 20 µg
Lane 5:
RAW 264.7 treated with 1000ng/ml BFA for 3h whole cell lysate at 20 µg
Lane 6:
RAW 264.7 treated with 100ng/ml lipopolysaccharide (LPS) for 4h then add 1000ng/ml BFA for another 3h whole cell lysate at 20 µg
Lane 7:
Human kidney tissue lysate at 20 µg
Lane 8:
A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution
Predicted band size: 35 kDa
Observed band size: 60 kDa
false
Exposure time: 180s
- WB
Lab
Western blot - Anti-Osteopontin antibody [RM1018] (AB283656)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
The multiple bands observed are likely due to expression of different isoforms, as well as glycosylation (PMID : 8761944; PMID : 11375993; PMID : 8535240; PMID : 12890718).
Exposure time : 2 min.
All lanes:
Western blot - Anti-Osteopontin antibody [RM1018] (ab283656) at 1/1000 dilution
Lane 1:
Human kidney lysate at 20 µg
Lane 2:
MDA-MB-435S (Human mammary gland ductal carcinoma melanocyte) whole cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 60 kDa
false
- WB
Lab
Western blot - Anti-Osteopontin antibody [RM1018] (AB283656)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Exposure time : 37 seconds.
All lanes:
Western blot - Anti-Osteopontin antibody [RM1018] (ab283656) at 1/1000 dilution
Lane 1:
RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg
Lane 2:
RAW 264.7 treated with 100ng/ml lipopolysaccharide (LPS) for 3h then add 300ng/ml BFA for another 3h whole cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 60 kDa
false
- cELISA
Supplier Data
Competitive ELISA - Anti-Osteopontin antibody [RM1018] (AB283656)
ELISA using ab283656 at varying antibody concentrations and antigen concentration at 1000 ng/ml. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Related conjugates and formulations (1)
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Anti-Osteopontin antibody [RM1018] - BSA and Azide free
Reactivity data
Product details
What is this antibody validated in?
Anti-Osteopontin antibody [RM1018] (ab283656) is a rabbit recombinant recombinant multiclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), ELISA in Human, Mouse, samples.
What is the molecular weight of Osteopontin?
Anti-Osteopontin [RM1018] (ab283656) specifically detects a band for Osteopontin (UniProt: P10451) at a molecular weight of 35kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [RM1018] also available for your convenience: ab283656, Carrier free - ab283669
What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:
- - The sensitivity of polyclonal antibodies by recognising multiple epitopes
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
View our range of recombinant multiclonal antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties and storage information
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Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.
Pathways
Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.
Product protocols
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Target data
Additional targets
Publications (26)
Recent publications for all applications. Explore the full list and refine your search
Cell biology and toxicology 41:99 PubMed40493284
2025
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Bioactive materials 51:293-305 PubMed40487243
2025
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Journal of biophotonics 18:e70051 PubMed40294940
2025
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Journal of inflammation (London, England) 22:4 PubMed39856697
2025
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Nutrients 16: PubMed39458447
2024
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International journal of molecular sciences 25: PubMed39409192
2024
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Journal of lipid research 65:100657 PubMed39326787
2024
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Regenerative biomaterials 11:rbae112 PubMed39323741
2024
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iScience 27:110801 PubMed39310777
2024
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Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 44:762-769 PubMed39066537
2024
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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