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AB307994

Anti-Osteopontin antibody [RP23040158]

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Anti-Osteopontin antibody [RP23040158] is a Rabbit Recombinant Multiclonal antibody that is used in Osteopontin Western Blot. Suitable for Human, Mouse, Rat samples.

Osteopontin (OPN) is a protein that plays a crucial role in cancer progression by promoting tumor growth, invasion, angiogenesis, and immune modulation. It is associated with poor prognosis and survival rates in various cancers, and its interaction with integrins and CD44 receptors, along with post-translational modifications, enhances its functional activities. OPN also contributes to immunosuppression in the tumor microenvironment, making it a potential therapeutic target.

View Alternative Names

BNSP, OPN, PSEC0156, SPP1, Osteopontin, Bone sialoprotein 1, Nephropontin, Secreted phosphoprotein 1, Urinary stone protein, Uropontin, SPP-1

1 Images
Western blot - Anti-Osteopontin antibody [RP23040158] (AB307994)
  • WB

Supplier Data

Western blot - Anti-Osteopontin antibody [RP23040158] (AB307994)

Western blot analysis was performed U-2-OS (Lane 1), Hep G2 (Lane 2), Mouse Liver (Lane 3) and Rat Brain (Lane 4) tissue extracts. The blots were probed with ab307994 at 1-2 µg/mL and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 0.4 µg/mL (1/2500 dilution). A 35 kDa band corresponding to Osteopontin (cleaved) was observed across cell lines tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was then performed.

All lanes:

Western blot - Anti-Osteopontin antibody [RP23040158] (ab307994) at 2 µg/mL

Lane 1:

U-2-OS cells at 30 µg

Lane 2:

Hep G2 cells at 30 µg

Lane 3:

Mouse liver at 30 µg

Lane 4:

Rat liver at 30 µg

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1/2500 dilution

Observed band size: 35 kDa

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Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040158

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SPP1.

P10451

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Reactivity data

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: 99.91% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Osteopontin also known as OPN or secreted phosphoprotein 1 (SPP1) is a glycoprotein with a molecular weight of approximately 44 to 75 kDa depending on its post-translational modifications. Osteopontin is characteristically expressed in bone but is also present in various body tissues including kidney liver and immune system cells. This multifunctional protein interacts with cell surface receptors such as integrins and CD44 playing an important role in cell signaling and extracellular matrix interactions. Researchers often study it using osteopontin western blot or osteopontin ELISA kit to understand its expression patterns and roles.
Biological function summary

Osteopontin affects cell adhesion migration and survival. It does not form part of a known large complex but interacts closely with its integrin receptors. Osteopontin modulates immune responses and aids in bone remodeling by affecting osteoclast and osteoblast functions. It is also influential in inflammation processes where it orchestrates the recruitment and activation of macrophages. The osteopontin function is significant in both physiological processes and pathologies marking it as an important protein to study in numerous biological contexts.

Pathways

Osteopontin is integral to both the bone remodeling and immune response pathways. It collaborates with proteins like osteocalcin and matrix metalloproteinases within these processes. In the bone remodeling pathway osteopontin mediates communication between osteoclasts and osteoblasts impacting bone resorption and formation. In the immune response pathway it coordinates with cytokines such as interleukin-6 to modulate immune cell activities. Its roles in these pathways indicate its multifunctional nature and its impact across different biological systems.

Osteopontin links to cancer and cardiovascular diseases. It facilitates tumor progression and metastasis by interacting with proteins such as vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9). In cardiovascular diseases osteopontin influences atherosclerosis development through interactions with inflammatory cytokines and contributes to plaque stability. These disease associations highlight the importance of osteopontin as both a therapeutic target and a biomarker for disease progression and prognosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Major non-collagenous bone protein that binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.. Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
See full target information SPP1
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