Anti-Osteoprotegerin antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteoprotegerin antibody (AB183910)

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labeling OPG with ab183910 at 1/500 dilution. Antigen Retrieval : EDTA based buffer, pH 8.0 for 15 min.

• WB

Supplier Data

Western blot - Anti-Osteoprotegerin antibody (AB183910)

Samples were separated by 10 % SDS. The signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Osteoprotegerin antibody (ab183910) at 1/1000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

Jurkat whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 46 kDa

true

• WB

Supplier Data

Western blot - Anti-Osteoprotegerin antibody (AB183910)

Samples were separated by 10 % SDS. The signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-Osteoprotegerin antibody (ab183910) at 1/1000 dilution

All lanes:

Human plasma at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 46 kDa

true

• WB

Supplier Data

Western blot - Anti-Osteoprotegerin antibody (AB183910)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-Osteoprotegerin antibody (ab183910) at 1/1000 dilution

Lane 1:

MG-63 whole cell extract at 30 µg

Lane 2:

MG-63 whole cell extract in conditioned medium at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 46 kDa

false

• WB

Supplier Data

Western blot - Anti-Osteoprotegerin antibody (AB183910)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-Osteoprotegerin antibody (ab183910) at 1/1500 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

Transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 46 kDa

false

• WB

Supplier Data

Western blot - Anti-Osteoprotegerin antibody (AB183910)

Samples were separated by 10 % SDS.

All lanes:

Western blot - Anti-Osteoprotegerin antibody (ab183910) at 1/500 dilution

Lane 1:

PC-12 whole cell extracts at 30 µg

Lane 2:

Rat2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 46 kDa

false

• WB

CiteAb

Western blot - Anti-Osteoprotegerin antibody (AB183910)

Osteoprotegerin western blot using anti-Osteoprotegerin antibody ab183910. Publication image and figure legend from Liu, W., Wang, Z., et al., 2019, Open Biol, PubMed 31088250.

ab183910 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab183910 please see the product overview.

Activation of mTORC1 in osteocytes stimulates RANKL expression and osteoclast formation in mice. (a) Representative images of TRAP staining of femora from 10-week-old control (DTCL) and Tsc1 CKO mice. The scale bars represent 50 μm. (b) Number of TRAP-positive osteoclasts (t-test, p = 0.0017, n = 6). (c) Western blot analysis of RANKL in bone lysates from DTCL and Tsc1 CKO mice (n = 6). (d–g) MLO-Y4 cells were infected with control shRNA lentivirus (shNC) and TSC1 shRNA lentivirus (ΔTSC1) for 72 h. (d) Representative images of lentivirus infection of MLO-Y4 cells. Scale bars, 50 μm. Western blot analysis of pS6, pS6K, RANKL and OPG (e) in MLO-Y4 cells infected with TSC1 shRNA lentivirus. qPCR analysis for (f) RANKL and (g) OPG mRNA levels (t-test, RANKL : p = 0.0054, OPG : p = 0.0171, n = 6). (h–j) MLO-Y4 cells were treated with 1 nM rapamycin (ΔR) and DMSO (vehicle) for 48 h, then cell lysates were subjected to immunoblot (h) and qPCR analysis for (i) RANKL and (j) OPG protein and mRNA levels, respectively (t-test, RANKL : p = 0.0003, OPG : p = 0.0220, n = 6). All experiments were repeated independently three times. Data are represented as mean ± s.d., *p < 0.05, **p < 0.01 and ***p < 0.001.

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