Rabbit Polyclonal Osteoprotegerin antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 31 publications. Immunogen corresponding to Synthetic Peptide within Human TNFRSF11B aa 1-100.
Images
Publications
Filter by
- Journal of clinical medicine 12:2023Jaw Bone Invasion of Oral Squamous Cell Carcinoma Is Associated with Osteoclast Count and Expression of Its Regulating Proteins in Patients and Organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWillem W B de Kort,Wisse E Haakma,Robert J J van Es,Debby Gawlitta,Else Driehuis,Merel Gansevoort,Stefan M WillemsPubMed 37762975
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e229852023A static magnetic field improves bone quality and balances the function of bone cells with regulation on iron metabolism and redox status in type 1 diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuanhuan Lv,Yijia Wang,Chenxiao Zhen,Junyu Liu,Xin Chen,Gejing Zhang,Wei Yao,Huijie Guo,Yunpeng Wei,Shenghang Wang,Jiancheng Yang,Peng ShangPubMed 37249350
- Evidence-based complementary and alternative medicine : eCAM 2023:41679062023Iridoid from Oliv. Exerts Antiarthritis Effects by Inhibiting the JAK2/STAT3 Signaling Pathway and .Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Dong Tang,Jian-Ying Wang,Yan Zhang,Xiao-Yun Chen,Lei Zhang,Ying YuanPubMed 37123081
- Advances in clinical and experimental medicine : official organ Wroclaw Medical University 32:245-2592022Prognostic relevance of clinicopathological factors in sporadic and syndromic odontogenic keratocysts: A comparative study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKonrad Kisielowski,Bogna Drozdzowska,Mariusz Szuta,Tomasz KaczmarzykPubMed 36226690
- Journal of orthopaedic translation 37:46-602022Fecal microbiota transplantation ameliorates bone loss in mice with ovariectomy-induced osteoporosis via modulating gut microbiota and metabolic function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan-Wei Zhang,Mu-Min Cao,Ying-Juan Li,Pan-Pan Lu,Guang-Chun Dai,Ming Zhang,Hao Wang,Yun-Feng RuiPubMed 36196151
- Oncology letters 23:1492022causes changes in the host's expression of cancer-associated miRNAs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Wang,Ning Wang,Ying Hui Zhao,Gang LuPubMed 35350589
- Bioactive materials 15:409-4252022Biocompatible reduced graphene oxide stimulated BMSCs induce acceleration of bone remodeling and orthodontic tooth movement through promotion on osteoclastogenesis and angiogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDelong Jiao,Jing Wang,Wenting Yu,Ke Zhang,Ning Zhang,Lingyan Cao,Xinquan Jiang,Yuxing BaiPubMed 35386350
- Journal of cellular and molecular medicine 26:60-742021LncRNA SNHG1 modulates adipogenic differentiation of BMSCs by promoting DNMT1 mediated Opg hypermethylation via interacting with PTBP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Yu,Meng-Sheng Song,Peng-Ze Rong,Xian-Jun Chen,Lin Shi,Cheng-Hao Wang,Qing-Jiang PangPubMed 34854215
- International journal of molecular sciences 22:2021Aesculetin Accelerates Osteoblast Differentiation and Matrix-Vesicle-Mediated Mineralization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWoojin Na,Min-Kyung Kang,Sin-Hye Park,Dong Yeon Kim,Su Yeon Oh,Moon-Sik Oh,Sohyun Park,Ii-Jun Kang,Young-Hee KangPubMed 34830274
- Frontiers in physiology 12:6977382021AdipoRon Promotes the Osseointegration of Dental Implants in Mice With Type 2 Diabetes Mellitus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBoRui Huang,Wei Bi,Yang Sun,Ruixue Li,Xingwen Wu,Youcheng YuPubMed 36632609
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TNFRSF11B aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link O00300
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
Alternative names
OCIF, OPG, TNFRSF11B, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin
Recommended products
Rabbit Polyclonal Osteoprotegerin antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 31 publications. Immunogen corresponding to Synthetic Peptide within Human TNFRSF11B aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TNFRSF11B aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link O00300
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Osteoprotegerin (OPG) also known as osteoclastogenesis inhibitory factor is a glycoprotein with a mass of approximately 60 kDa. It acts as a soluble decoy receptor for receptor activator of nuclear factor kappa-Β ligand (RANKL) and is encoded by the TNFRSF11B gene. OPG is mainly expressed in osteoblasts fibroblasts and endothelial cells. Its expression can be found in diverse tissues throughout the body including the liver and lungs.
- Biological function summary
OPG inhibits the differentiation and activation of osteoclasts by binding to RANKL preventing its interaction with the receptor RANK on the surface of osteoclast progenitor cells. As part of a non-classical signaling pathway OPG plays a significant role in the regulation of bone remodeling and maintenance by controlling bone resorption. This activity preserves bone density and structure by limiting excessive osteoclastic activity.
- Pathways
OPG operates within the RANK/RANKL/OPG signaling axis which is important for bone metabolism. It counteracts the bone-resorption pathway driven by the RANKL-RANK interaction inhibiting osteoclastogenesis. This axis involves interactions with other proteins such as RANK RANKL and TRAIL where TRAIL’s involvement hints towards a broader immune regulation role for OPG.
- Associated diseases and disorders
OPG has a significant association with conditions such as osteoporosis and vascular calcification. In osteoporosis an imbalance in the RANKL/OPG ratio can lead to excessive bone loss due to increased osteoclast activity. In vascular calcification OPG appears to play a protective role likely linked with regulatory mechanisms involving RANKL and TRAIL. Understanding these interactions opens potential therapeutic avenues particularly in managing bone-related and cardiovascular conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteoprotegerin antibody (ab183910)
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft labeling OPG with ab183910 at 1/500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0 for 15 min.
Western blot - Anti-Osteoprotegerin antibody (ab183910)
10% SDS-PAGE
All lanes: Western blot - Anti-Osteoprotegerin antibody (ab183910) at 1/1000 dilution
All lanes: Mouse heart lysate at 50 µg
Predicted band size: 46 kDa
Western blot - Anti-Osteoprotegerin antibody (ab183910)
10% SDS-PAGE
All lanes: Western blot - Anti-Osteoprotegerin antibody (ab183910) at 1/1000 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 46 kDa
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Osteoprotegerin antibody (ab183910)
Osteoprotegerin western blot using anti-Osteoprotegerin antibody ab183910. Publication image and figure legend from Liu, W., Wang, Z., et al., 2019, Open Biol, PubMed 31088250.
ab183910 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab183910 please see the product overview.
Activation of mTORC1 in osteocytes stimulates RANKL expression and osteoclast formation in mice. (a) Representative images of TRAP staining of femora from 10-week-old control (DTCL) and Tsc1 CKO mice. The scale bars represent 50 µm. (b) Number of TRAP-positive osteoclasts (t-test, p = 0.0017, n = 6). (c) Western blot analysis of RANKL in bone lysates from DTCL and Tsc1 CKO mice (n = 6). (d–g) MLO-Y4 cells were infected with control shRNA lentivirus (shNC) and TSC1 shRNA lentivirus (ΔTSC1) for 72 h. (d) Representative images of lentivirus infection of MLO-Y4 cells. Scale bars, 50 µm. Western blot analysis of pS6, pS6K, RANKL and OPG (e) in MLO-Y4 cells infected with TSC1 shRNA lentivirus. qPCR analysis for (f) RANKL and (g) OPG mRNA levels (t-test, RANKL: p = 0.0054, OPG: p = 0.0171, n = 6). (h–j) MLO-Y4 cells were treated with 1 nM rapamycin (ΔR) and DMSO (vehicle) for 48 h, then cell lysates were subjected to immunoblot (h) and qPCR analysis for (i) RANKL and (j) OPG protein and mRNA levels, respectively (t-test, RANKL: p = 0.0003, OPG: p = 0.0220, n = 6). All experiments were repeated independently three times. Data are represented as mean ± s.d., *p < 0.05, **p < 0.01 and ***p < 0.001.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com