Rabbit Polyclonal Osteoprotegerin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 86 publications.
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- BMC oral health 23:6392023Comparative analysis of anchorage strength and histomorphometric changes after implantation of miniscrews in adults and adolescents: an experimental study in Beagles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Zhao,TingTing Jia,Zhiqiang WangPubMed 37670309
- Biomedicines 11:2023Therapeutic Potential of BMP7 in the Treatment of Osteoporosis Caused by the Interaction between Inflammation and Corticosteroids in Inflammatory Bowel Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIvana Smoljan,Dijana Detel,Suncica Buljevic,Igor Erjavec,Ivana MarićPubMed 37626658
- Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 52:786-7942023Hypoxia caused by unilateral nasal obstruction decreases mandibular density in rats through inhibition of Cyp1a1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYixin Xu,Yixin Wang,Hui Xiao,Yongming LiPubMed 37489271
- Life (Basel, Switzerland) 13:2023Secukinumab and Black Garlic Downregulate OPG/RANK/RANKL Axis and Devitalize Myocardial Interstitial Fibrosis Induced by Sunitinib in Experimental Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHoda E Mohamad,Mervat E Asker,Mohamed A Shaheen,Nourhan M Baraka,Omer I Fantoukh,Abdulaziz Alqahtani,Alaa E Salama,Yasmin K MahmoudPubMed 36836664
- International journal of dentistry 2022:73989242022Inhibits Alveolar Bone Destruction in a Rat Model with Lipopolysaccharide (LPS)-Induced Periodontitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFumie Shiba,Hisako Furusho,Takashi Takata,Rika Shimizu,Mutsumi MiyauchiPubMed 36794024
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e22053362022Nitric Oxide-Releasing Bioinspired Scaffold for Exquisite Regeneration of Osteoporotic Bone via Regulation of Homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Kyu Lee,Da-Seul Kim,So-Yeon Park,Seung-Woon Baek,Ji-Won Jung,Tae-Hyung Kim,Dong Keun HanPubMed 36581472
- Journal of clinical medicine 12:2022Effect of Mechanical Stimuli and Zoledronic Acid on the Femoral Bone Morphology in Rats with Obesity and Limited Mobility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNazar M Kostyshyn,Siemowit Muszyński,Ewa Tomaszewska,Agnieszka Tomczyk-Warunek,Iwona Puzio,Piotr DobrowolskiPubMed 36614859
- Medicina (Kaunas, Lithuania) 58:2022Osteoclast and Sclerostin Expression in Osteocytes in the Femoral Head with Risedronate Therapy in Patients with Hip Fractures: A Retrospective Comparative Study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHwan-Hee Lee,Eun-Yong Choi,Hyun-Sik Jun,Young-Yul KimPubMed 36363523
- Nutrients 14:2022A Low-Phenylalanine-Containing Whey Protein Hydrolysate Stimulates Osteogenic Activity through the Activation of p38/Runx2 Signaling in Osteoblast Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Bu,Yuting Ren,Songfeng Yu,Jiexia Zheng,Ling Liu,Peilong Sun,Jianping Wu,Kai YangPubMed 35956311
- Journal of periodontology 94:244-2552022Abaloparatide outperforms teriparatide in protecting against alveolar bone loss in experimental periodontitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Li,Yuran Qian,Hui Qiao,Weiyi Pan,Liang Xie,Yu LiPubMed 35892139
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Sheep | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Cow, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Sheep, Cow, Pig | Dilution info - | Notes - |
Associated Products
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6 products for Alternative Product
Target data
Function
Acts as a decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis.
Alternative names
OCIF, OPG, TNFRSF11B, Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin
Recommended products
Rabbit Polyclonal Osteoprotegerin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 86 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Osteoprotegerin (OPG) also known as osteoclastogenesis inhibitory factor is a glycoprotein with a mass of approximately 60 kDa. It acts as a soluble decoy receptor for receptor activator of nuclear factor kappa-Β ligand (RANKL) and is encoded by the TNFRSF11B gene. OPG is mainly expressed in osteoblasts fibroblasts and endothelial cells. Its expression can be found in diverse tissues throughout the body including the liver and lungs.
- Biological function summary
OPG inhibits the differentiation and activation of osteoclasts by binding to RANKL preventing its interaction with the receptor RANK on the surface of osteoclast progenitor cells. As part of a non-classical signaling pathway OPG plays a significant role in the regulation of bone remodeling and maintenance by controlling bone resorption. This activity preserves bone density and structure by limiting excessive osteoclastic activity.
- Pathways
OPG operates within the RANK/RANKL/OPG signaling axis which is important for bone metabolism. It counteracts the bone-resorption pathway driven by the RANKL-RANK interaction inhibiting osteoclastogenesis. This axis involves interactions with other proteins such as RANK RANKL and TRAIL where TRAIL’s involvement hints towards a broader immune regulation role for OPG.
- Associated diseases and disorders
OPG has a significant association with conditions such as osteoporosis and vascular calcification. In osteoporosis an imbalance in the RANKL/OPG ratio can lead to excessive bone loss due to increased osteoclast activity. In vascular calcification OPG appears to play a protective role likely linked with regulatory mechanisms involving RANKL and TRAIL. Understanding these interactions opens potential therapeutic avenues particularly in managing bone-related and cardiovascular conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Image courtesy of Carl Hobbs (Kings College London, United Kingdom)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteoprotegerin antibody (ab73400)
IHC-P image of Osteoprotegerrin staining on Rat kidney sections using ab73400 (1:4000). The sections were deparaffinized and subjected to heat mediated antigen retrieval using citric acid. The sections were blocked using 1% BSA for 10 mins at 21°C. ab73400 was diluted 1:4000 in TBS buffer (containing BSA and Azide) and sections were then incubated with ab73400 for 2 hours at 21°C. The secondary antibody used was Biotin conjugated Goat polyclonal to Rabbit IgG (1:250).
Western blot - Anti-Osteoprotegerin antibody (ab73400)
Osteoprotegerin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
All lanes: Western blot - Anti-Osteoprotegerin antibody (ab73400) at 1 µg/mL
Lane 1: SK N BE (Human neuroblastoma) Whole Cell Lysate at 10 µg
Lane 2: K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 105 kDa, 14 kDa, 48 kDa
Exposure time: 8min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Osteoprotegerin antibody (ab73400)
IHC image of Osteoprotegerin staining in Human Normal Kidney FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73400, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunocytochemistry/ Immunofluorescence - Anti-Osteoprotegerin antibody (ab73400)
ab73400 staining Osteoprotegerin in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab73400 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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