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AB284856

Anti-OSTF1 antibody [EP15457] - BSA and Azide free

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Rabbit Monoclonal OSTF1 antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

View Alternative Names

Osteoclast-stimulating factor 1, OSTF1

7 Images
Flow Cytometry (Intracellular) - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)

This data was developed using ab202901, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling OSTF1 with ab202901 at 1/100 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)

This data was developed using ab202901, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling OSTF1 with ab202901 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)

This data was developed using ab202901, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling OSTF1 with ab202901 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : - ab202901 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)

This data was developed using ab202901, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling OSTF1 with ab202901 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
The negative controls are as follows : -
-ve control 1 : - ab202901 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

Immunoprecipitation - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)
  • IP

Supplier Data

Immunoprecipitation - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)

This data was developed using ab202901, the same antibody clone in a different buffer formulation.

OSTF1 was immunoprecipitated from 1 mg of MCF7 (Human breast adenocarcinoma cell line) whole cell lysate with ab202901 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab202901 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : MCF7 whole cell lysate 10 μg (Input).

Lane 2 : ab202901 IP in MCF7 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab202901 in MCF7 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-OSTF1 antibody [EP15457] (<a href='/en-us/products/primary-antibodies/ostf1-antibody-ep15457-ab202901'>ab202901</a>)

Predicted band size: 24 kDa

false

Western blot - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)
  • WB

Supplier Data

Western blot - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)

This data was developed using ab202901, the same antibody clone in a different buffer formulation.

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-OSTF1 antibody [EP15457] (<a href='/en-us/products/primary-antibodies/ostf1-antibody-ep15457-ab202901'>ab202901</a>) at 1/5000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 3min

Western blot - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)
  • WB

Supplier Data

Western blot - Anti-OSTF1 antibody [EP15457] - BSA and Azide free (AB284856)

This data was developed using ab202901, the same antibody clone in a different buffer formulation.

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-OSTF1 antibody [EP15457] (<a href='/en-us/products/primary-antibodies/ostf1-antibody-ep15457-ab202901'>ab202901</a>) at 1/5000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 10 µg

Lane 2:

HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 10 µg

Lane 3:

MCF7 (Human breast carcinoma cell line) whole cell lysate at 10 µg

Lane 4:

Human fetal liver lysate at 10 µg

Lane 5:

Human tonsil lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 24 kDa

Observed band size: 24 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP15457

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra), IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Secondary Antibodies

AB150191

Rabbit Anti-Human IgM mu chain (Alexa Fluor® 647)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit Anti-Human IgM mu chain (Alexa Fluor® 647) (AB150191)

  • 0
  • 0
Primary Antibodies

AB198861

Anti-FAT3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FAT3 antibody (AB198861)

  • 0
  • 0
Secondary Antibodies

AB99811

Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP)

VeriBlot

Indirect ELISA - Mouse monoclonal [JDC-12] Anti-Human lambda light chain (HRP) (AB99811)

  • 5
  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab284856 is the carrier-free version of ab202901

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OSTF1 also known as Osteoclast-Stimulating Factor 1 is an important protein involved in the regulation of bone resorption. It has a molecular weight of approximately 60 kDa. OSTF1 is widely expressed in bone tissues where it influences the activity of osteoclasts. Researchers have identified it as a cytoplasmic protein that plays a role in signaling processes. OSTF1 functions as an adapter protein linking other proteins involved in cellular functions related to bone metabolism.
Biological function summary

OSTF1 interacts with cytoskeleton-associated proteins to enhance osteoclast activity. It coordinates the assembly of protein complexes essential for cell movement and actin cytoskeleton organization. OSTF1 is known to engage in complex formations with proteins such as Src a tyrosine kinase that facilitates its regulatory activities in osteoclasts. This interaction highlights OSTF1's role in the dynamics of cell adhesion and mobility which are important for bone remodeling processes.

Pathways

OSTF1 plays a role in the RANK/RANKL/OPG signaling pathway an important pathway for bone homeostasis. It partners with the Src protein in this pathway influencing osteoclast differentiation and activation. Besides OSTF1 is linked to the integrin signaling pathway through its interactions with cytoskeleton components which is equally significant for cell adhesion and survival. By mediating these interactions OSTF1 helps modulate the balance between formation and breakdown of bone matrix.

OSTF1 has associations with osteoporosis and Paget’s disease of bone. In osteoporosis the deregulation of OSTF1 influences abnormal osteoclast activity leading to increased bone resorption and fragility. In Paget’s disease OSTF1 may interact with other proteins like TNF receptor-associated factor 6 (TRAF6) affecting the excessive breakdown and formation of bone. The involvement of OSTF1 in these conditions makes it a target of interest for therapeutic interventions in bone diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Induces bone resorption, acting probably through a signaling cascade which results in the secretion of factor(s) enhancing osteoclast formation and activity.
See full target information Osteoclast-stimulating factor 1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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