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AB121327

Anti-OSTM1 antibody

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(2 Publications)

Rabbit Polyclonal OSTM1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human OSTM1 aa 200-300.

View Alternative Names

GL, HSPC019, UNQ6098/PRO21201, OSTM1, Osteopetrosis-associated transmembrane protein 1, Chloride channel 7 beta subunit

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-OSTM1 antibody (AB121327)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-OSTM1 antibody (AB121327)

Immunocytochemistry/immunofluorescence analysis of A431 cells labelling OSTM1 with ab121327 shows positive staining in the nucleus, cytoplasm and vesicles.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSTM1 antibody (AB121327)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSTM1 antibody (AB121327)

ab121327, at 1/200, staining OSTM1 in paraffin embedded Human liver tissue by Immunohistochemistry.

Western blot - Anti-OSTM1 antibody (AB121327)
  • WB

Unknown

Western blot - Anti-OSTM1 antibody (AB121327)

All lanes:

Western blot - Anti-OSTM1 antibody (ab121327) at 1/250 dilution

Lane 1:

Negative control (vector only transfected HEK293T lysate)

Lane 2:

OSTM1-transfected HEK293T over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa))

Predicted band size: 37 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human OSTM1 aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q86WC4

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Alternative Products

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OSTM1 also known as Osteopetrosis Associated Transmembrane Protein 1 is a protein with a molecular mass of approximately 47 kDa. It primarily resides in lysosomal and endosomal membranes. OSTM1 is highly expressed in osteoclasts cells responsible for bone resorption. It is important for osteoclast differentiation and function ensuring the breakdown of the bone matrix. OSTM1 forms a complex with other membrane proteins assisting in ion homeostasis within the osteoclast environment.
Biological function summary

The OSTM1 protein contributes to maintaining cellular ionic balance and is a part of the chloride channel complex. This complex is important for proper osteoclast activity as it facilitates the transport of ions necessary for creating an acidic environment. The protein participates in chloride-ion channel transport affecting the acidification of resorptive vacuoles. This action supports bone resorption enabling bone remodeling and growth.

Pathways

OSTM1 plays an important role in the RANK-RANKL signaling pathway which regulates osteoclast differentiation and activation. OSTM1 interacts with proteins like CLCN7 another chloride channel protein within this pathway. Through this interaction it assists in the formation and maintenance of acidic compartments in osteoclasts essential for bone mineral dissolution. Therefore OSTM1 is integral to the processes of bone turnover and mineral homeostasis.

Mutations or malfunctions in OSTM1 are directly linked to the development of autosomal recessive osteopetrosis a condition characterized by increased bone density and associated complications. OSTM1 dysfunction leads to impaired osteoclast activity resulting in defective bone resorption. Additionally a connection exists between OSTM1 and CLCN7 where defects in either protein can manifest similar clinical conditions highlighting their collaborative role in maintaining skeletal health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for osteoclast and melanocyte maturation and function.
See full target information OSTM1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 32:1907-1919 PubMed40629165

2025

Glycerol mediates crosstalk between metabolism and trafficking through the golgin Imh1.

Applications

Unspecified application

Species

Unspecified reactive species

Wan-Yun Chiu,Yi-Hsun Wang,Ming-Chieh Lin,Chun-Chi Lai,Chia-Jung Yu,Fang-Jen S Lee

Current issues in molecular biology 47: PubMed39852172

2025

Oncogene OSTM1 Promotes Gastric-Cancer Metastasis by Modulating the Metastatic Microenvironment Through Altered Tumor-Cell Autocrine Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yucheng Tang,Yi Guo,Jiangyi Feng,Ziwei Wang
View all publications
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Product promise

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