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AB203629

Anti-OSTM1 antibody

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(1 Publication)

Rabbit Polyclonal OSTM1 antibody. Suitable for WB, IHC-P and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human OSTM1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

GL, HSPC019, UNQ6098/PRO21201, OSTM1, Osteopetrosis-associated transmembrane protein 1, Chloride channel 7 beta subunit

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSTM1 antibody (AB203629)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OSTM1 antibody (AB203629)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat kidney tissue labeling OSTM1 with ab203629 at 1/200 dilution. DAB staining.

Western blot - Anti-OSTM1 antibody (AB203629)
  • WB

Supplier Data

Western blot - Anti-OSTM1 antibody (AB203629)

All lanes:

Western blot - Anti-OSTM1 antibody (ab203629) at 1/200 dilution

All lanes:

human colon carcinoma lysates

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

Predicted band size: 37 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human OSTM1 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q86WC4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe please use dilutions of 1/50 - 1/200.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OSTM1 also known as Osteopetrosis Associated Transmembrane Protein 1 is a protein with a molecular mass of approximately 47 kDa. It primarily resides in lysosomal and endosomal membranes. OSTM1 is highly expressed in osteoclasts cells responsible for bone resorption. It is important for osteoclast differentiation and function ensuring the breakdown of the bone matrix. OSTM1 forms a complex with other membrane proteins assisting in ion homeostasis within the osteoclast environment.
Biological function summary

The OSTM1 protein contributes to maintaining cellular ionic balance and is a part of the chloride channel complex. This complex is important for proper osteoclast activity as it facilitates the transport of ions necessary for creating an acidic environment. The protein participates in chloride-ion channel transport affecting the acidification of resorptive vacuoles. This action supports bone resorption enabling bone remodeling and growth.

Pathways

OSTM1 plays an important role in the RANK-RANKL signaling pathway which regulates osteoclast differentiation and activation. OSTM1 interacts with proteins like CLCN7 another chloride channel protein within this pathway. Through this interaction it assists in the formation and maintenance of acidic compartments in osteoclasts essential for bone mineral dissolution. Therefore OSTM1 is integral to the processes of bone turnover and mineral homeostasis.

Mutations or malfunctions in OSTM1 are directly linked to the development of autosomal recessive osteopetrosis a condition characterized by increased bone density and associated complications. OSTM1 dysfunction leads to impaired osteoclast activity resulting in defective bone resorption. Additionally a connection exists between OSTM1 and CLCN7 where defects in either protein can manifest similar clinical conditions highlighting their collaborative role in maintaining skeletal health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for osteoclast and melanocyte maturation and function.
See full target information OSTM1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biology of reproduction 97:302-312 PubMed29044427

2017

Genomic identification, expression profiling, and functional characterization of CatSper channels in the bovine.

Applications

Unspecified application

Species

Unspecified reactive species

Gillian P Johnson,Anne-Marie English,Sinead Cronin,David A Hoey,Kieran G Meade,Sean Fair
View all publications
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Product promise

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