AB53233

Anti-Otoferlin antibody [13A9]

Name: Anti-Otoferlin antibody [13A9] (ab53233) | Abcam
Brand: Abcam Limited
SKU: AB53233
Price: 470 USD
Availability: InStock
Rating: 4 (3 reviews)

(3 Reviews)

(49 Publications)

Anti-Otoferlin antibody [13A9] (ab53233) is a mouse monoclonal antibody detecting Otoferlin in Flow Cytometry, ICC/IF. Suitable for Human, Mouse.

- Over 30 publications
- Trusted since 2007

View Alternative Names

FER1L2, OTOF, Otoferlin, Fer-1-like protein 2

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Otoferlin antibody [13A9] (AB53233)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Otoferlin antibody [13A9] (AB53233)

ICC/IF analysis of mouse inner ear tissue labeling Otoferlin with ab181781. At 30 days postnatal, Otoferlin is detected in the hair cells of the crista ampullaris (CA) , utricular macula (UM) and saccularmacula (SM).

Flow Cytometry - Anti-Otoferlin antibody [13A9] (AB53233)

Flow Cyt

Unknown

Flow Cytometry - Anti-Otoferlin antibody [13A9] (AB53233)

Overlay histogram showing SHSY-5Y cells stained with ab53233 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53233, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

13A9

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Protein within Human OTOF aa 1-400. The exact immunogen used to generate this antibody is proprietary information.

Q9HC10

Specificity

This antibody reacts specifically with human Otoferlin protein (220 kDa).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Otoferlin antibody [13A9] (ab53233) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.

Trusted by the scientific community
Anti-Otoferlin [13A9] (ab53233) was first used in a scientific publication in 2007 and has been cited over 30 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The otoferlin protein also known as OTOF plays an important mechanical role in the auditory system by serving as an important synaptic vesicle component for neurotransmitter release in inner hair cells of the cochlea. It has a molecular mass of approximately 230 kDa. Otoferlin is expressed primarily in the cochlea particularly in the sensory hair cells essential for hearing. Its functionality hinges on its capacity to serve as a calcium-sensor facilitating the rapid exocytosis of neurotransmitter-filled vesicles at the synaptic ribbon.

Biological function summary

Otoferlin contributes to sound perception by coordinating the timely release of synaptic vesicles that transmit auditory signals to the brain. It is part of a complex network within the auditory pathway interacting with other proteins important for synaptic function including synaptotagmin. Significant for its role in calcium-binding otoferlin participates in the fine-tuning of synaptic transmission ensuring efficient signaling required for precise auditory processing.

Pathways

Otoferlin is integral to the auditory signaling pathways especially the cochlear nerve pathway responsible for conveying sound information to the brain. It actively participates in vesicular trafficking and synaptic vesicle cycle ensuring high fidelity auditory signal transduction. Otoferlin works closely with proteins such as synaptophysin and syntaxin which are involved in the vesicle fusion process highlighting its role in facilitating efficient neurotransmitter release.

Otoferlin has a significant association with auditory processing conditions. Mutations in the otoferlin gene lead to severe-to-profound hereditary hearing loss specifically a form of non-syndromic autosomal recessive hearing impairment (DFNB9). Through this condition otoferlin has connections to proteins involved in synaptic vesicle recycling malfunctions contributing to auditory neuropathy. Additionally otoferlin-related dysfunction implicates it in conditions affecting synaptic transmission stability which are vital considerations in therapeutic strategies for hearing loss.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes (By similarity).

See full target information OTOF

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Publications (49)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Methods & clinical development 33:101577 PubMed41036103

2025

Functional, sustained recovery of hearing in Otoferlin-deficient mice using DB-OTO, a hair-cell-specific AAV-based gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Yoojin Chung,Seth D Koehler,Sarah Cancelarich,Tyler M Gibson,Gabriela Pregernig,Lars Becker,Quynh-Anh Artinian,Joseph W Goodliffe,Ning Pan,Tera M Quigley,Arun Senapati,Peter G Slade,Lillian M Smith,Kathy S So,Xichun Zhang,C Eduardo Corrales,Peter Weber,Lynn E Macdonald,Brian Zambrowicz,Aris N Economides,Christos A Kyratsous,Joseph C Burns,Adam T Palermo,Leah R Sabin,John Lee,Vassili Valayannopoulos,Jonathon P Whitton,Meghan C Drummond

JCI insight 9: PubMed39641274

2024

In vivo AAV9-Myo7a gene rescue restores hearing and cholinergic efferent innervation in inner hair cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew P O'Connor,Ana E Amariutei,Alice Zanella,Sarah A Hool,Adam J Carlton,Fanbo Kong,Mauricio Saenz-Roldan,Jing-Yi Jeng,Marie-José Lecomte,Stuart L Johnson,Saaid Safieddine,Walter Marcotti

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2304551 PubMed38810137

2024

AAV-mediated Gene Cocktails Enhance Supporting Cell Reprogramming and Hair Cell Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Liyan Zhang,Xin Chen,Xinlin Wang,Yinyi Zhou,Yuan Fang,Xingliang Gu,Ziyu Zhang,Qiuhan Sun,Nianci Li,Lei Xu,Fangzhi Tan,Renjie Chai,Jieyu Qi

The Journal of neuroscience : the official journal of the Society for Neuroscience 44: PubMed38688721

2024

Revisiting the Potency of Tbx2 Expression in Transforming Outer Hair Cells into Inner Hair Cells at Multiple Ages In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenghong Bi,Minhui Ren,Yu Zhang,Shunji He,Lei Song,Xiang Li,Zhiyong Liu

Research (Washington, D.C.) 7:0341 PubMed38665848

2024

Engineering of the AAV-Compatible Hair Cell-Specific Small-Size Myo15 Promoter for Gene Therapy in the Inner Ear.

Applications

Unspecified application

Species

Unspecified reactive species

Shao Wei Hu,Jun Lv,Zijing Wang,Honghai Tang,Hui Wang,Fang Wang,Daqi Wang,Juan Zhang,Longlong Zhang,Qi Cao,Yuxin Chen,Ziwen Gao,Yu Han,Wuqing Wang,Geng-Lin Li,Yilai Shu,Huawei Li

Molecular therapy. Nucleic acids 35:102135 PubMed38404504

2024

Hair cell-specific Myo15 promoter-mediated gene therapy rescues hearing in DFNB9 mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Wang,MengZhao Xun,Honghai Tang,Jingjing Zhao,Shaowei Hu,Longlong Zhang,Jun Lv,Daqi Wang,Yuxin Chen,Jianping Liu,Geng-Lin Li,Wuqing Wang,Yilai Shu,Huawei Li

Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology head and neck surgery 38:49-56 PubMed38297849

2024

[Study on gene therapy for DPOAE and ABR threshold changes in adult mice].

Applications

Unspecified application

Species

Unspecified reactive species

Zijing Wang,Qi Cao,Shaowei Hu,Xintai Fan,Jun Lv,Hui Wang,Wuqing Wang,Huawei Li,Yilai Shu

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2306788 PubMed38189623

2024

AAV-Mediated Gene Therapy Restores Hearing in Patients with DFNB9 Deafness.

Applications

Unspecified application

Species

Unspecified reactive species

Jieyu Qi,Fangzhi Tan,Liyan Zhang,Ling Lu,Shanzhong Zhang,Yabo Zhai,Yicheng Lu,Xiaoyun Qian,WenXiu Dong,Yinyi Zhou,Ziyu Zhang,Xuehan Yang,Lulu Jiang,Chaorong Yu,Jiancheng Liu,Tian Chen,Lianqiu Wu,Chang Tan,Sijie Sun,Huaien Song,Yilai Shu,Lei Xu,Xia Gao,Huawei Li,Renjie Chai

Molecular & cellular proteomics : MCP 23:100704 PubMed38128648

2023

Proteomic Analysis Reveals the Composition of Glutamatergic Organelles of Auditory Inner Hair Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andreia P Cepeda,Momchil Ninov,Jakob Neef,Iwan Parfentev,Kathrin Kusch,Ellen Reisinger,Reinhard Jahn,Tobias Moser,Henning Urlaub

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2306201 PubMed38014592

2023

Preclinical Efficacy And Safety Evaluation of AAV-OTOF in DFNB9 Mouse Model And Nonhuman Primate.

Applications

Unspecified application

Species

Unspecified reactive species

Jieyu Qi,Liyan Zhang,Fangzhi Tan,Yang Zhang,Yinyi Zhou,Ziyu Zhang,Hongyang Wang,Chaorong Yu,Lulu Jiang,Jiancheng Liu,Tian Chen,Lianqiu Wu,Shanzhong Zhang,Sijie Sun,Shan Sun,Ling Lu,Qiuju Wang,Renjie Chai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Otoferlin antibody [13A9]

Immunocytochemistry/ Immunofluorescence - Anti-Otoferlin antibody [13A9] (AB53233)

Flow Cytometry - Anti-Otoferlin antibody [13A9] (AB53233)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (49)

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

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