Anti-Otoferlin antibody [13A9] (ab53233) is a mouse monoclonal antibody that is used to detect Otoferlin in Flow Cytometry, ICC/IF. Suitable for Human, Mouse samples.
- Over 30 publications
- Trusted since 2007
Images
![Immunocytochemistry/ Immunofluorescence - Anti-Otoferlin antibody [13A9] (AB53233), expandable thumbnail](https://content.abcam.com/products/images/otoferlin-antibody-13a9-ab53233--immunocytochemistry-immunofluorescence-img107654.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Otoferlin antibody [13A9] (AB53233)")
![Flow Cytometry - Anti-Otoferlin antibody [13A9] (AB53233), expandable thumbnail](https://content.abcam.com/products/images/otoferlin-antibody-13a9-ab53233--flow-cytometry-img127471.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Otoferlin antibody [13A9] (AB53233)")
Publications
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- Molecular therapy. Methods & clinical development 31:1011542023Preclinical evaluation of the efficacy and safety of AAV1-hOTOF in mice and nonhuman primates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLonglong Zhang,Hui Wang,Mengzhao Xun,Honghai Tang,Jinghan Wang,Jun Lv,Biyun Zhu,Yuxin Chen,Daqi Wang,Shaowei Hu,Ziwen Gao,Jianping Liu,Zheng-Yi Chen,Bing Chen,Huawei Li,Yilai ShuPubMed 38027066
- International journal of molecular sciences 24:2023Hyperacusis in the Adult -KO Mouse Model of Fragile X Syndrome: The Therapeutic Relevance of Cochlear Alterations and BKCa Channels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCeleste Ferraguto,Yohan Bouleau,Thibault Peineau,Didier Dulon,Susanna PietropaoloPubMed 37511622
- Nature communications 14:30852023Cochlear transcript diversity and its role in auditory functions implied by an otoferlin short isoform.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuihui Liu,Hongchao Liu,Longhao Wang,Lei Song,Guixian Jiang,Qing Lu,Tao Yang,Hu Peng,Ruijie Cai,Xingle Zhao,Ting Zhao,Hao WuPubMed 37248244
- Life science alliance 5:2021MANF supports the inner hair cell synapse and the outer hair cell stereocilia bundle in the cochlea.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKuu Ikäheimo,Anni Herranen,Vilma Iivanainen,Tuuli Lankinen,Antti A Aarnisalo,Ville Sivonen,Kashyap A Patel,Korcan Demir,Mart Saarma,Maria Lindahl,Ulla PirvolaPubMed 34815294
- Developmental neurobiology 81:905-9382021Neuronal development in the cochlea of a nonhuman primate model, the common marmoset.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMakoto Hosoya,Masato Fujioka,Ayako Y Murayama,Hiroyuki Ozawa,Hideyuki Okano,Kaoru OgawaPubMed 34545999
- Frontiers in aging neuroscience 13:7564492021Synaptic Release Potentiation at Aging Auditory Ribbon Synapses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesThibault Peineau,Séverin Belleudy,Susanna Pietropaolo,Yohan Bouleau,Didier DulonPubMed 34733152
- Frontiers in neuroscience 15:7236582021Complete Elimination of Peripheral Auditory Input Before Onset of Hearing Causes Long-Lasting Impaired Social Memory in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Guo,Yang Li,Jiao Liu,Shusheng Gong,Ke LiuPubMed 34385906
- Development (Cambridge, England) 148:2021Mosaic CRISPR-stop enables rapid phenotyping of nonsense mutations in essential genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuangqin Wang,Chao Li,Shunji He,Zhiyong LiuPubMed 33558388
- Frontiers in molecular neuroscience 13:6000512021Overloaded Adeno-Associated Virus as a Novel Gene Therapeutic Tool for Otoferlin-Related Deafness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVladan Rankovic,Christian Vogl,Nele M Dörje,Iman Bahader,Carlos J Duque-Afonso,Anupriya Thirumalai,Thomas Weber,Kathrin Kusch,Nicola Strenzke,Tobias MoserPubMed 33488357
- Journal of cell science 133:2020AP180 promotes release site clearance and clathrin-dependent vesicle reformation in mouse cochlear inner hair cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJana Kroll et. al.PubMed 31843760
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Protein within Human OTOF aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HC10
Reactivity data
Select an application| Flow Cyt | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Expected | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes (By similarity).
Alternative names
FER1L2, OTOF, Otoferlin, Fer-1-like protein 2
Recommended products
Anti-Otoferlin antibody [13A9] (ab53233) is a mouse monoclonal antibody that is used to detect Otoferlin in Flow Cytometry, ICC/IF. Suitable for Human, Mouse samples.
- Over 30 publications
- Trusted since 2007
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Constituents: 2% Sucrose, 1.21% Tris, 0.75% Glycine
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Protein within Human OTOF aa 1-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HC10
- Clone number
- 13A9
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody reacts specifically with human Otoferlin protein (220 kDa).
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Otoferlin antibody [13A9] (ab53233) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
Trusted by the scientific community
Anti-Otoferlin [13A9] (ab53233) was first used in a scientific publication in 2007 and has been cited over 30 times in peer-reviewed journals.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The otoferlin protein also known as OTOF plays an important mechanical role in the auditory system by serving as an important synaptic vesicle component for neurotransmitter release in inner hair cells of the cochlea. It has a molecular mass of approximately 230 kDa. Otoferlin is expressed primarily in the cochlea particularly in the sensory hair cells essential for hearing. Its functionality hinges on its capacity to serve as a calcium-sensor facilitating the rapid exocytosis of neurotransmitter-filled vesicles at the synaptic ribbon.
- Biological function summary
Otoferlin contributes to sound perception by coordinating the timely release of synaptic vesicles that transmit auditory signals to the brain. It is part of a complex network within the auditory pathway interacting with other proteins important for synaptic function including synaptotagmin. Significant for its role in calcium-binding otoferlin participates in the fine-tuning of synaptic transmission ensuring efficient signaling required for precise auditory processing.
- Pathways
Otoferlin is integral to the auditory signaling pathways especially the cochlear nerve pathway responsible for conveying sound information to the brain. It actively participates in vesicular trafficking and synaptic vesicle cycle ensuring high fidelity auditory signal transduction. Otoferlin works closely with proteins such as synaptophysin and syntaxin which are involved in the vesicle fusion process highlighting its role in facilitating efficient neurotransmitter release.
- Associated diseases and disorders
Otoferlin has a significant association with auditory processing conditions. Mutations in the otoferlin gene lead to severe-to-profound hereditary hearing loss specifically a form of non-syndromic autosomal recessive hearing impairment (DFNB9). Through this condition otoferlin has connections to proteins involved in synaptic vesicle recycling malfunctions contributing to auditory neuropathy. Additionally otoferlin-related dysfunction implicates it in conditions affecting synaptic transmission stability which are vital considerations in therapeutic strategies for hearing loss.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Immunocytochemistry/ Immunofluorescence - Anti-Otoferlin antibody [13A9] (ab53233), expandable thumbnail](https://content.abcam.com/products/images/otoferlin-antibody-13a9-ab53233--immunocytochemistry-immunofluorescence-img107654.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Otoferlin antibody [13A9] (ab53233)")
Immunocytochemistry/ Immunofluorescence - Anti-Otoferlin antibody [13A9] (ab53233)
ICC/IF analysis of mouse inner ear tissue labeling Otoferlin with ab181781. At 30 days postnatal, Otoferlin is detected in the hair cells of the crista ampullaris (CA) , utricular macula (UM) and saccularmacula (SM).
![Flow Cytometry - Anti-Otoferlin antibody [13A9] (ab53233), expandable thumbnail](https://content.abcam.com/products/images/otoferlin-antibody-13a9-ab53233--flow-cytometry-img127471.jpg "Flow Cytometry - Anti-Otoferlin antibody [13A9] (ab53233)")
Flow Cytometry - Anti-Otoferlin antibody [13A9] (ab53233)
Overlay histogram showing SHSY-5Y cells stained with ab53233 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53233, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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