AB254267

Anti-OTP antibody [EPR22178-17]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal OTP antibody. Suitable for IHC-P and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Homeobox protein orthopedia, OTP

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue labeling OTP with ab254267 at 1/4000 dilution (0.113μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control : No staining in human lung squamous cell carcinoma (PMID : 26927888) is observed. The expression of the target is developmental stage-specific and type-specific for lung cancer. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemical analysis of paraffin-embedded human lung carcinoid tissue labeling OTP with ab254267 at 1/4000 dilution (0.113μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human lung carcinoid (PMID : 30284410, 29316326) is observed. The expression of the target is developmental stage-specific and type-specific for lung cancer. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemical analysis of paraffin-embedded human lung small cell carcinoma tissue labeling OTP with ab254267 at 1/4000 dilution (0.113μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human lung small cell carcinoma (PMID : 26927888) is observed. The expression of the target is developmental stage-specific and type-specific for lung cancer. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling OTP with ab254267 at 1/4000 dilution (0.113μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Negative control : No staining in human lung (PMID : 26927888) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Carrier free

Anti-OTP antibody [EPR22178-17] - BSA and Azide free
578 PE

PE Anti-OTP antibody [EPR22178-17]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-OTP antibody [EPR22178-17]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-OTP antibody [EPR22178-17]
660 APC

APC Anti-OTP antibody [EPR22178-17]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-OTP antibody [EPR22178-17]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-OTP antibody [EPR22178-17]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-OTP antibody [EPR22178-17]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22178-17

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

OTP also known as orthopedia homeobox is a transcription factor involved in the development and differentiation of neuroendocrine cells. It belongs to the homeobox family of proteins which are characterized by a specific DNA-binding domain. OTP typically weighs around 35 kDa. This protein is mainly expressed in the central nervous system and neuroendocrine tissues particularly within the hypothalamus.

Biological function summary

OTP plays an important role in the regulation of gene expression during the development of specific neuronal cell types. It is not part of a larger complex but interacts with various other transcription factors to influence cell fate decisions. OTP's regulatory actions are essential during embryonic development where it directs the differentiation and specialization of neurons.

Pathways

OTP is significant in the development pathways of the nervous system. It participates in neurogenesis particularly influencing the hypothalamic lineage. OTP interacts with proteins such as Nkx2.1 and Sim1 which are also transcription factors that guide the specification of neuroendocrine neurons. These interactions highlight OTP’s involvement in complex genetic regulatory networks essential for proper nervous system function.

OTP shows relevance to certain neurodevelopmental disorders and tumors. Abnormal OTP expression links to the development of medullary thyroid carcinoma a type of cancer affecting thyroid gland cells. Furthermore OTP's expression patterns can aid in distinguishing certain tumor types in neuropathology. Researchers study its interaction with proteins like RET which is also implicated in thyroid cancer to better understand and treat these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Probably involved in the differentiation of hypothalamic neuroendocrine cells.

See full target information OTP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 16:5618-5633 PubMed38499392

2024

Predicting the prognosis of glioma patients with TERT promoter mutations and guiding the specific immune profile of immune checkpoint blockade therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Wenpeng Cao,Jinzhi Lan,Chujiao Hu,Jinping Kong,Limin Xiang,Zhixue Zhang,Yating Sun,Zhirui Zeng,Shan Lei

Archives of pathology & laboratory medicine 147:1446-1450 PubMed36800547

2023

Molecular Characterization of Testicular Mesothelioma and the Role of Asbestos as a Causative Factor.

Applications

Unspecified application

Species

Unspecified reactive species

Ashleigh Jean Hocking,Elaine May Thomas,Sarita Prabhakaran,Alexandra Jolley,Susan Lesley Woods,Matthew J Soeberg,Sonja Klebe

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-OTP antibody [EPR22178-17]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-OTP antibody [EPR22178-17] (AB254267)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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